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Development of a specimen holder with a light source to realize highly efficient x-ray irradiation on biological cells

Research Project

Project/Area Number 21540517
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plasma science
Research InstitutionJapan Atomic Energy Agency

Principal Investigator

KADO Masataka  独立行政法人日本原子力研究開発機構, 量子ビーム応用研究部門, 研究副主幹 (30360431)

Co-Investigator(Renkei-kenkyūsha) ISHINO Masahiko  独立行政法人日本原子力研究開発機構, 量子ビーム応用研究部門, 研究副主幹 (80360410)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsプラズマ応用 / X線顕微鏡 / 軟X線顕微鏡 / 極薄膜ターゲット / 生きたままの細胞 / X線瞬間撮像 / マルチカラーイメージング / 細胞内小器官 / レーザープラズマ / 水の窓
Research Abstract

We have developed a new specimen holder with a thin filmed gold target and observed live biological cells with flash exposure by realizing highly efficient x-ray irradiation. Comparing phase contrast images, fluorescent images, and soft x-ray images of the same biological cells directory, fine structures of cellular organelles have been analyzed. As a result strong correlation between cytoskeleton and mitochondria has been found and chromatin structures have been observed.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (35 results)

All 2012 2011 2010 2009 Other

All Journal Article (9 results) (of which Peer Reviewed: 3 results) Presentation (23 results) Remarks (3 results)

  • [Journal Article] Development of a specimen holder combined with ultra thin film laser plasma x-ray source for compact contact-type soft x-ray microscope to observe hydrated living biological cells2011

    • Author(s)
      石野雅彦、加道雅孝, 他
    • Journal Title

      Proceeding of SPIE

      Volume: Vol.8139 Pages: 81390R-81390R

    • DOI

      10.1117/12.892864

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Observation of Organelles in Leydig Cells by Contact Soft X-Ray Microscopy with a Laser Plasma X-Ray Source2011

    • Author(s)
      加道雅孝, 他
    • Journal Title

      AIP Confference Proceeding

      Volume: Vol.1365 Pages: 391-394

    • DOI

      10.1063/1.3625385

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Flash imaging of fine structures of cellular organelles by contact x-ray microscopy with a high intensity laser plasma x-ray source2010

    • Author(s)
      加道雅孝, 他
    • Journal Title

      Proceeding of SPIE

      Volume: Vol.8139 Pages: 81390O-81390O

    • DOI

      10.1117/12.892438

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Observations of the intense soft x-ray emissions from ultra thin Au films irradiated with high contrast laser pulses2010

    • Author(s)
      石野雅彦、加道雅孝, 他
    • Journal Title

      Proceedings of SPIE

      Volume: Vol.7589 Pages: 75891B-75891B

    • DOI

      10.1117/12.839945

    • Related Report
      2011 Final Research Report
  • [Journal Article] Observation of actin filaments in Leydig cells with a contact-type soft x-ray microscope with laser plasma x-ray source2010

    • Author(s)
      加道雅孝、石野雅彦, 他
    • Journal Title

      電気学会論文誌C

      Volume: Vol.130 Pages: 1774-1778

    • NAID

      10026694109

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Observation of Actin Filaments in Leydig Cells with a Contact-type Soft X-ray Microscope with Laser Plasma X-ray Source2010

    • Author(s)
      加道雅孝, 他
    • Journal Title

      IEEJ Transactions on Electronics, Information and Systems

      Volume: 130 Issue: 10 Pages: 1774-1778

    • DOI

      10.1541/ieejeiss.130.1774

    • NAID

      10026694109

    • ISSN
      0385-4221, 1348-8155
    • Related Report
      2011 Final Research Report
  • [Journal Article] Observation of actin filaments in Leydig cells with a contact-type soft x-ray microscope with laser plasma x-ray source2010

    • Author(s)
      M.Kado, M.Ishino, 他
    • Journal Title

      電気学会論文誌C

      Volume: 130 Pages: 1774-1778

    • NAID

      10026694109

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Observations of the intense soft x-ray emissions from ultra thin Au films irradiated with high contrast laser pulses2010

    • Author(s)
      M.Ishino, M.Kado, 他
    • Journal Title

      Proceedings of SPIE

      Volume: 7589

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Development of a soft x-ray plasma camera with a Fresnel zone plate to image laser produced plasmas2009

    • Author(s)
      加道雅孝, 他
    • Journal Title

      Journal of Physics : Conference Series 186

      Pages: 12031-12034

    • Related Report
      2009 Annual Research Report
  • [Presentation] 極薄膜ターゲットを用いた高輝度軟X線源の開発2012

    • Author(s)
      加道雅孝
    • Organizer
      日本物理学会第67回年次大会
    • Place of Presentation
      兵庫県西宮市
    • Year and Date
      2012-03-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 極薄膜ターゲットを用いた高輝度軟X線源の開発2012

    • Author(s)
      加道雅孝
    • Organizer
      日本物理学会第67回年次大会
    • Place of Presentation
      西宮市
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 水の窓波長X線を用いた軟X線顕微鏡による生細胞のその場観察2012

    • Author(s)
      加道雅孝
    • Organizer
      電顕技術開発若手研究部会第3回ワークショップ
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2012-01-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 水の窓波長X線を用いた軟X線顕微鏡による生細胞のその場観察2012

    • Author(s)
      加道雅孝
    • Organizer
      電顕技術開発若手研究部会第3回ワークショップ
    • Place of Presentation
      名古屋市(招待講演)
    • Year and Date
      2012-01-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] レーザープラズマ軟X線を用いた密着型顕微鏡による細胞内小器官の観察2011

    • Author(s)
      加道雅孝
    • Organizer
      平成23年度レーザー励起X線源とその応用研究会
    • Place of Presentation
      静岡県熱海市
    • Year and Date
      2011-12-09
    • Related Report
      2011 Final Research Report
  • [Presentation] レーザープラズマ軟X線を用いた密着型顕微鏡による細胞内小器官の観察2011

    • Author(s)
      加道雅孝
    • Organizer
      平成23年度レーザー励起X線源とその応用研究会
    • Place of Presentation
      熱海市
    • Year and Date
      2011-12-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] レーザープラズマX線を用いた密着型軟X線顕微鏡による細胞内器官の撮像2011

    • Author(s)
      加道雅孝
    • Organizer
      第11回X線結像光学シンポジウム
    • Place of Presentation
      仙台市(招待講演)
    • Year and Date
      2011-11-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] レーザープラズマ軟X線顕微鏡による細胞内器官の構造解析II2011

    • Author(s)
      加道雅孝
    • Organizer
      日本物理学会2011年秋季大会
    • Place of Presentation
      富山県富山市
    • Year and Date
      2011-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] レーザープラズマ軟X線顕微鏡による細胞内器官の構造解析II2011

    • Author(s)
      加道雅孝
    • Organizer
      日本物理学会2011年秋季大会
    • Place of Presentation
      富山市
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Flash imaging of fine structures of cellular organelles by contact x-ray microscopy with a high intensity laser plasma x-ray source2011

    • Author(s)
      加道雅孝
    • Organizer
      SPIE 2011 Optics+Photonics
    • Place of Presentation
      サンディエゴ(米国)
    • Year and Date
      2011-08-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of a specimen holder combined with ultra thin film laser plasma x-ray source for compact contact-type soft x-ray microscope to observe hydrated living biological cells2011

    • Author(s)
      石野雅彦
    • Organizer
      SPIE 2011 Optics+Photonics
    • Place of Presentation
      サンディエゴ(米国)
    • Year and Date
      2011-08-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Flash imaging of fine structures of cellular organelles by contact x-ray microscopy with a high intensity laser plasma x-ray source2011

    • Author(s)
      加道雅孝, 他
    • Organizer
      SPIE 2011 Optics+Photonics
    • Place of Presentation
      米国サンディエゴ
    • Year and Date
      2011-08-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of a specimen holder combined with ultra thin film laser plasma x-ray source for compact contact-type soft x-ray microscope to observe hydrated living biological cells2011

    • Author(s)
      石野雅彦、加道雅孝
    • Organizer
      SPIE 2011 Optics+Photonics
    • Place of Presentation
      米国サンディエゴ
    • Year and Date
      2011-08-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] レーザープラズマ軟X線による細胞内器官の観察2011

    • Author(s)
      加道雅孝
    • Organizer
      レーザー学会専門委員会第5回委員会
    • Place of Presentation
      大阪府吹田市
    • Year and Date
      2011-06-24
    • Related Report
      2011 Final Research Report
  • [Presentation] レーザープラズマ軟X線による細胞内器官の観察2011

    • Author(s)
      加道雅孝
    • Organizer
      レーザー学会専門委員会第5回委員会
    • Place of Presentation
      吹田市(招待講演)
    • Year and Date
      2011-06-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] レーザープラズマX線源を用いた密着型軟X線顕微鏡による細胞内器官の観察2011

    • Author(s)
      加道雅孝
    • Organizer
      第24回日本放射光学会年会・放射光科学合同シンポジウム、サテライトシンポジウム
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2011-01-11
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 極薄膜ターゲットを用いた高輝度軟X線源の開発2010

    • Author(s)
      加道雅孝
    • Organizer
      平成22年度レーザープラズマ粒子加速研究会
    • Place of Presentation
      静岡県浜松市
    • Year and Date
      2010-12-07
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Recent progress on plasma x-ray laser and its application at JAEA2010

    • Author(s)
      加道雅孝
    • Organizer
      3rd Photonics and OptoElectronics Meetings
    • Place of Presentation
      武漢(中国)
    • Year and Date
      2010-11-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Recent progress on plasma x-ray laser and its application at JAEA2010

    • Author(s)
      加道雅孝
    • Organizer
      3rd Photonics and OptoElectronics Meetings
    • Place of Presentation
      Wuhan, China
    • Year and Date
      2010-11-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Observation of Organelles in Leydig Cells by Contact Soft X-Ray Microscopy with a Laser Plasma X-Ray Source2010

    • Author(s)
      加道雅孝
    • Organizer
      10th International Conference on X-Ray Microscopy
    • Place of Presentation
      シカゴ(米国)
    • Year and Date
      2010-08-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Observation of Organelles in Leydig Cells by Contact Soft X-Ray Microscopy with a Laser Plasma X-Ray Source2010

    • Author(s)
      加道雅孝
    • Organizer
      10th International Conference on X-Ray Microscopy
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2010-08-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] 軟X線による生体試料イメージングII細胞内器官のイメージング2010

    • Author(s)
      加道雅孝
    • Organizer
      日本顕微鏡学会第66回学術講演会
    • Place of Presentation
      愛知県名古屋市
    • Year and Date
      2010-05-25
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Observations of the intense soft x-ray emissions from ultra thin Au films irradiated with high contrast laser pulses2010

    • Author(s)
      石野雅彦
    • Organizer
      SPIE Photonics West 2010
    • Place of Presentation
      サンフランシスコ(米国)
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Remarks] (1)プレス発表:「初めて見た生きた細胞の超微細構造の観察に成功-夢の顕微鏡:レーザープラズマ軟X線顕微鏡の開発で実現-」、2011年8月17日、大阪科学・大学記者クラブ

    • URL

      http://www.jaea.go.jp/02/press2011/p11081701/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks] (2)平成23年度大阪ニュークリアサイエンス協会賞(応用研究・開発部門)受賞「レーザー励起X線源を用いた軟X線顕微鏡による細胞内小器官のその場観察技術の開発」

    • URL

      http://homepage2.nifty.com/onsa/jusyousya.htm

    • Related Report
      2011 Final Research Report
  • [Remarks] プレス発表

    • URL

      http://www.jaea.go.jp/02/press2011/p11081701/index.html

    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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