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Developments of selective detection methods of oligosaccharides bound to receptor molecule

Research Project

Project/Area Number 21550092
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionMeisei University

Principal Investigator

TASHIRO Mitsuru  明星大学, 理工学部, 准教授 (40315750)

Co-Investigator(Kenkyū-buntansha) YOSHIMURA Etsuro  東京大学, 大学院・農学生命科学研究科, 教授 (10130303)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords生物学的分析 / 高精度選択的分析 / レセブター・リガンド複合体 / 選択的検出 / 核磁気共鳴 / 分子間相互作用 / 高感度化・高精度化 / タンパク質 / リガンド
Research Abstract

Various new methods to detect interactions of the protein-ligandcomplex have been developed. Although sample solutions containing 100% D_2O weregenerally used in the previous studies, new methods, which can handle 95% H_2O and 5% D_2O, were required on the point of easy sample preparation with avoiding sample loss. Effective and sensitive detection of ligand signals, resonating close to water, was alsoperformed in this research project. These methods are expected to be useful in thechemical and pharmaceutical fields.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (41 results)

All 2011 2010 2009 Other

All Journal Article (30 results) (of which Peer Reviewed: 30 results) Presentation (10 results) Book (1 results)

  • [Journal Article] Characterization of the complex formation of 1, 6-anhydro-maltose and potassium ions using NMR spectroscopy and single-crystal X-ray crystallography2011

    • Author(s)
      T. Fujimoto, T. Kato, Y. Usui, O. Kamo, K. Furihata, K. Tsubono, T. Kato, T. Machinami and M. Tashiro
    • Journal Title

      Carbohydr. Res

      Volume: 346 Pages: 1991-1996

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective J-resolved-HMQC-1 and-2, new methods for measuring proton-proton coupling constants in strongly coupled spin systems2011

    • Author(s)
      K. Furihata and M. Tashiro
    • Journal Title

      Magn. Reson. Chem

      Volume: 49 Pages: 459-463

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] BIRD-J-resolved HMBC and BIRD-high resolution-HMBC pulse sequences for measuring heteronuclear long range coupling constants and proton-proton spin coupling constants in the complicated spin systems2011

    • Author(s)
      Kazuo Furihata, M. Tashiro and H. Seto
    • Journal Title

      Magn. Reson. Chem

      Volume: 49 Pages: 53-58

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The binding of aluminum to mugineic acid and related compounds as studied by potentiometric titration2011

    • Author(s)
      E. Yoshimura, H. Kohde, S. Mori, R. C. Hider
    • Journal Title

      Biometals

      Volume: 24 Pages: 723-727

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synechocystis ferredoxin-NADP(+) oxidoreductase is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin2011

    • Author(s)
      J. Sato, K. Takeda, R. Nishiyama, T. Watanabe, M. Abo, E. Yoshimura, J. Nakagawa, A. Abe, S. Kawasaki, Y. Niimura
    • Journal Title

      Biometals

      Volume: 24 Pages: 311-321

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cadmium(II)-stimulated enzyme activation of Arabidopsis thaliana phytochelatin synthase2011

    • Author(s)
      S. Ogawa, T. Yoshidomi, E. Yoshimura
    • Journal Title

      J. Inorg. Biochem

      Volume: 105 Pages: 111-117

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the complex formation of 1,6-anhydro-β-maltose and potassium ions using NMR spectroscopy and single-crystal X-ray crystallography2011

    • Author(s)
      T.Fujimoto, T.Kato, Y.Usui, O.Kamo, K.Furihata, K.Tsubono, T.Kato, T.Machinami, M.Tashiro
    • Journal Title

      Carbohydr.Res.

      Volume: 346 Issue: 13 Pages: 1991-1996

    • DOI

      10.1016/j.carres.2011.06.017

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective J-resolved-HMQC-1 and-2, new methods for measuring proton-proton coupling constants in strongly coupled spin systems2011

    • Author(s)
      K.Furihata, M.Tashiro
    • Journal Title

      Magn.Reson.Chem.

      Volume: 49 Issue: 8 Pages: 459-463

    • DOI

      10.1002/mrc.2778

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BIRD-J-resolved HMBC and BIRD-high resolution-HMBC pulse sequences for measuring heteronuclear long range coupling constants and proton-proton spin coupling constants in the complicated spin systems2011

    • Author(s)
      Kazuo Furihata, M.Tashiro, H.Seto
    • Journal Title

      Magn.Reson.Chem.

      Volume: 49 Issue: 2 Pages: 53-58

    • DOI

      10.1002/mrc.2702

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NMRの高磁場化および高感度化の進歩2011

    • Author(s)
      田代充
    • Journal Title

      ぶんせき

      Volume: 2 Pages: 102-103

    • NAID

      10027906709

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An effective pulse Sequence for detecting a ligand binding with a protein receptor using a WET Sequence and the repeated Z-filters2010

    • Author(s)
      K. Furihata, S. Shimotakahara, Y. Shibusawa and M. Tashiro
    • Journal Title

      Anal. Sci

      Volume: 26 Pages: 1107-1110

    • NAID

      10026785945

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a descrete peroxide dianion, O_2+2-, in a two sodium-(1, 6-anhydro-β-maltose)_2-peroxide complex2010

    • Author(s)
      T. Kato, T. Fujimoto, A. Tsutsui, M. Tashiro, Y. Mitsutsuka, and T. Machinami
    • Journal Title

      Chem. Lett

      Volume: 39 Pages: 136-137

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] High resolution-HMBC(HR-HMBC), a new method for measuring heteronuclear long range coupling constants2010

    • Author(s)
      K. Furihata, M. Tashiro and H. Seto
    • Journal Title

      Magn. Reson. Chem

      Volume: 48 Pages: 179-183

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Micro total bioassay system for ingested substances : assessment of intestinal absorption, hepatic metabolism, and bioactivity2010

    • Author(s)
      Y. Imura, K. Sato, E. Yoshimura
    • Journal Title

      Anal. Chem

      Volume: 82 Pages: 9983-9988

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aluminum stress increases carbon-centered radicals in soybean roots2010

    • Author(s)
      M. Abo, H. Yonehara, E. Yoshimura
    • Journal Title

      J. Plant Physiol

      Volume: 167 Pages: 1316-1319

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-12010

    • Author(s)
      S. Ogawa, E. Yoshimura
    • Journal Title

      Anal. Biochem

      Volume: 402 Pages: 200-202

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An effective pulse Sequence for detecting a ligand binding with a protein receptor using a WET Sequence and the repeated Z-filters2010

    • Author(s)
      K.Furihata, S.Shimotakahara, Y.Shibusawa, M.Tashiro
    • Journal Title

      Anal.Sci.

      Volume: 26 Pages: 1107-1110

    • NAID

      10026785945

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High resolution-HMBC(HR-HMBC), a new method for measuring heteronuclear long range coupling constants2010

    • Author(s)
      K.Furihata, M.Tashiro, H.Seto
    • Journal Title

      Magn.Reson.Chem. 48

      Pages: 179-183

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a descrete peroxide dianion, O_2^<2->, in a two sodium-(1, 6-anhydro-β-maltose)_2-peroxide complex2010

    • Author(s)
      T.Kato, T.Fujimoto, A.Tsutsui, M.Tashiro, Y.Mitsutsuka, T.Machinami
    • Journal Title

      Chem.Lett. 39

      Pages: 136-137

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective J-resolved-HMBC, an efficient method for measuring heteronuclear long range coupling constants2009

    • Author(s)
      K. Furihata, M. Tashiro and H. Seto
    • Journal Title

      Magn. Reson. Chem

      Volume: 47 Pages: 814-818

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of WET sequence for detection of the ligand signals resonating close to water2009

    • Author(s)
      K. Furihata, S. Shimotakahara, Y. Shibusawa and M. Tashiro
    • Journal Title

      Magn. Reson. Chem

      Volume: 47 Pages: 971-976

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the complex formation of 1, 6-anhydro-β-maltotriose with potassium using 1^H and 39^K NMR spectroscopy2009

    • Author(s)
      T. Kato, K. Tsubono, O. Kamo, T. Kato, K. Furihata, T. Fujimoto, T. Machinami and M. Tashiro
    • Journal Title

      Magn. Reson. Chem

      Volume: 47 Pages: 948-952

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A microfluidic system to evaluate intestinal absorption2009

    • Author(s)
      Y. Imura, Y. Asano, K. Sato, E. Yoshimura
    • Journal Title

      Anal. Sci

      Volume: 25 Pages: 1403-1407

    • NAID

      10026780359

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Revision of analytical conditions for determining ligand molecules specific to soft metal ions using dequenching of copper(I)-bathocuproine disulfonate as a detection system2009

    • Author(s)
      S. Ogawa, R. Ichiki, M. Abo, E. Yoshimura
    • Journal Title

      Anal. Chem

      Volume: 81 Pages: 9199-9200

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of phytochelatin synthase produced by the primitive red alga Cyanidioschyzon merolae2009

    • Author(s)
      Y. Osaki, T. Shirabe, H. Nakanishi, T. Wakagi, E. Yoshimura
    • Journal Title

      Metallomics

      Volume: 1 Pages: 353-358

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the complex formation of 1, 6-anhydro-β-maltotriose with potassium using ^1H and ^<39>K NMR spectroscopy2009

    • Author(s)
      T.Kato, K.Tsubono, O.Kamo, T.Kato, K.Furihata, T.Fujimoto, T.Machinami, M.Tashiro
    • Journal Title

      Magn.Reson.Chem. 47

      Pages: 948-952

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of WET sequence for detection of the ligand signals resonating close to water2009

    • Author(s)
      K.Furihata, S.Shimotakahara, Y.Shibusawa, M.Tashiro
    • Journal Title

      Magn.Reson.Chem. 47

      Pages: 971-976

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective J-resolved-HMBC, an efficient method for measuring heteronuclear long range coupling constants2009

    • Author(s)
      K.Furihata, M.Tashiro, H.Seto
    • Journal Title

      Magn.Reson.Chem. 47

      Pages: 814-818

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of three dopamine molecules bound to α-synuclein : Implication of oligomerization at the initial stage

    • Author(s)
      S. Shimotakahara, Y. Shiroyama, T. Fujimoto, M. Akai, T. Onoue, H. Seki, S. Kado, T. Machinami, Y. Shibusawa, K. Ueda and Mitsuru Tashiro
    • Journal Title

      J. Biophy. Chem

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of three dopamine molecules bound to α-synuclein : Implication of oligomerization at the initial stage

    • Author(s)
      S.Shimotakahara, Y.Shiroyama, T.Fujimoto, M.Akai, T.Onoue, H.Seki, S.Kado, T.Machinami, Y.Shibusawa, K.Ueda, Mitsuru Tashiro
    • Journal Title

      J.Biophy.Chem.

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Complex formation of the sugar 1, 6-anhydro-maltose with sodium ions as probed by NMR spectroscopy2011

    • Author(s)
      田代充、吉村悦郎
    • Organizer
      3^<rd> International Symposium on Metallomics
    • Place of Presentation
      Munster, Germany
    • Related Report
      2011 Final Research Report
  • [Presentation] An effective pulse Sequence for detecting a ligand binding with a protein receptor using a WET Sequence and the repeated Z-filters2011

    • Author(s)
      田代充、下高原櫻子、降旗一夫
    • Organizer
      52^<th> Experimental NMR Conference
    • Place of Presentation
      CA, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Complex formation of the sugar 1,6-anhydro-β-maltose with sodium ions as probed by NMR spectroscopy2011

    • Author(s)
      田代充、吉村悦郎
    • Organizer
      The 3^<rd> International Symposium on Metallomics
    • Place of Presentation
      ミュンスター大学、ドイツ
    • Related Report
      2011 Annual Research Report
  • [Presentation] An effective pulse Sequence for detecting a ligand binding with a protein receptor using a WET Sequence and the repeated Z-filters2011

    • Author(s)
      田代充、下高原櫻子、降旗一夫
    • Organizer
      52^<th> Experimental NMR Conference
    • Place of Presentation
      カリフォルニア、USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of the complex formation of 1, 6-anhydro sugars with potassium using^<1> H and^<39> K NMR spectroscopy2010

    • Author(s)
      田代充、町並智也、藤本崇、加藤隆之
    • Organizer
      The 2010 International Chemical Congress of Pacific Basin Societies(PACIFICHEM 2010)
    • Place of Presentation
      Honolulu, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of gold nanoparticles conjugated with 2-mercaptoethanol using NMR pectroscopy2010

    • Author(s)
      田代充、吉村悦郎
    • Organizer
      Challenges in Inorganic and Materials Chemistry(ISACS3)
    • Place of Presentation
      香港、中国
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of the complex formation of 1, 6-anhydro sugars with potassium using 1^H and ^<39>K NMR spectroscopy2010

    • Author(s)
      田代充、町並智也、藤本崇、加藤隆之
    • Organizer
      The 2010 International Chemical Congress of Pacific Basin Societies(PACIFICHEM 2010)
    • Place of Presentation
      Honolulu, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characterization of gold nanoparticles conjugated with 2-mercaptoethanol using NMR spectroscopy2010

    • Author(s)
      田代充、吉村悦郎
    • Organizer
      Challenges in Inorganic and Materials Chemistry(ISACS3)
    • Place of Presentation
      香港、中国
    • Related Report
      2010 Annual Research Report
  • [Presentation] NMRによる1,6-anhydro-β-maltotriose-K^+の相互作用解析2009

    • Author(s)
      田代充, 加藤敏代, 藤本崇, 加藤隆之, 鴨修, 坪野浩二, 町並智也
    • Organizer
      第70回分析化学討論会
    • Place of Presentation
      和歌山大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] Practical use of WaterLOGSY and STD Techniques for Observing a Ligand Binding with a Protein Receptor2009

    • Author(s)
      M.Tashiro, K.Furihata, S.Shimotakahara
    • Organizer
      50^<th> ENC Conference
    • Place of Presentation
      Asilomar, CA, USA
    • Related Report
      2009 Annual Research Report
  • [Book] 分析化学実技シリーズ 機器分析編・3 NMR2009

    • Author(s)
      田代充, 加藤敏代
    • Total Pages
      239
    • Publisher
      共立出版
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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