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Study of the interrelationship between Legionella pneumophila and free-living amoeba under the condition of natural water temperature to solving legionaires' disease

Research Project

Project/Area Number 21560573
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionToho University

Principal Investigator

OHNO Akira  東邦大学, 医学部, 講師 (40223903)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords土壌 / 水環境 / Legionella pneumphila / Acanthamoeba castellanii / Acanthamoeba polyphaga / Haltomannella vermiformis / TaqMan real-time PCR / EMA Taqman real-time PCR / VNC / 河川水 / Legionella pneumophila / Acanthamoeba castellaniii / エチジウムモノアザイド処理リアルタイムPCR / 温度 / VNC(5)Acanthamoeba polyphaga / Haltomanella vermiformis / Real time PCR / BacLight染色
Research Abstract

It was demonstrated that L. pneumophila shifted into a VNC(Viable but not Culturable) mode inside the Acanthamoeba castellanii cells at a temperature of 15 degrees C by the combination of cultural method, real time PCR(RTPCR) and EMA treated real time PCR(EMA RTPCR), and by transmission electron microscope observation. On the other hand, the shift into VNC state was not observed in Acanthamoeba polyphaga and Haltomannella vermiformis. Culturable L. pneumophila cells were not detected in periodic survey of river water at fixed-sampling points targeting two major rivers in the Tokyo area. A few viable Legionella cells were detected by the combination of RTPCR and EMA RTPCR, however the numbers of viable cells were approximately-same without relation to season. The detection of free-living amoeba(FLA) by PCR showed that the genus Haltomanella was predominant in the sampling points and A. castellanii was not detected. The absence of FLA species that the interrelationship with L. pneumophila is affected by water temperature or few chances to encounter host amoeba by flow of the river might be cause of this result.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (6 results)

All 2011 2009

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results)

  • [Journal Article] レジオネラから覗く生物進化環境微生物の世界2009

    • Author(s)
      大野章
    • Journal Title

      日本温泉科学会雑誌

      Volume: 59巻 Pages: 179-183

    • NAID

      10026259020

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] Relationship between Acanthamoeba castellanii and Legionella pneumophila at a temperature below 20 degree C2011

    • Author(s)
      Akira Ohno, Hisashi Mochizuki, Naoyuki Kato, Yoshikazu Ishii, Kazuhiro Tateda, Takashi Suematsu, Yasuhisa Inoue, Keizo Yamaguchi
    • Organizer
      International Union of Microbiological World of Microbes 2011
    • Place of Presentation
      Sapporo Convention Center (Sapporo)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Relationship between Acanthamoeba castellanii and Legionella pneumophila at a temperature below 20 degree C2011

    • Author(s)
      Ohno A, Mochizuki H, Kato N, Ishii Y, Tateda K, Suematsu K, Inoue Y and Yamaguchi K
    • Organizer
      International Union of Microbiological Word of Micrbes
    • Place of Presentation
      Convention Center, Sappro, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Interrelationship between Legionella pneumophila and free-living amoeba at a low temperature2009

    • Author(s)
      Ohno A, Kato N, Yamaguchi K
    • Organizer
      The conference LEGIONELLA 2009
    • Place of Presentation
      Paris
    • Year and Date
      2009-10-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] レジオネラから覗く生物進化環境微生物の世界2009

    • Author(s)
      大野章
    • Organizer
      第62回日本温泉科学学会総会
    • Place of Presentation
      京都
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Interrelationship between Legionella pneumophila and free-living amoeba at a low temperature2009

    • Author(s)
      Ohno A, Kato N, Yamaguchi K
    • Organizer
      The conference LEGIONELLA
    • Place of Presentation
      Paris, France
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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