• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Fusion of affinity techniques for protein concentration and novel proteome analysis

Research Project

Project/Area Number 21560810
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionKyoto Institute of Technology

Principal Investigator

KISHIMOTO Michimasa  京都工芸繊維大学, 工芸科学研究科, 教授 (00144436)

Co-Investigator(Kenkyū-buntansha) KUMADA Youichi  京都工芸繊維大学, 工芸科学研究科, 助教 (70452373)
Co-Investigator(Renkei-kenkyūsha) NOMURA Akinari  京都大学, 医学研究科, 助教 (20402901)
IWATA Hiroo  京都大学, 再生医科学研究所, 教授 (30160120)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsアフィニティー濃縮 / プロテオーム解析 / ペプチド合成 / 医療診断 / 抗体医薬
Research Abstract

For the detection of minor protein or small amout of protein, which are vey useful for cancer and the other adult deseases, several approach is proposed using the peptide affinity technology for concentration of the minor protein by washing out the major protein. For the evaluation and promotion of these technology, we tried to develop the treatment of high concentration of standard protein and the graphic analysis method of the 2-DE gel image. These technolgy were applied for the scFv production to identify the culture phase and screening of proteins, which attached specifically to the surface of plastics.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (14 results)

All 2011 2010 2009 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (9 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Dynamic analysis of the silver staining gel image of 2-DE for protein quantification2010

    • Author(s)
      M. Kishimoto, Y. Tatsumi, N. Tamesui, Y. Kumada, J. Horiuchi, K. Okumura
    • Journal Title

      Journal of Electrophoresis

      Volume: Vol.51 Pages: 1-7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic analysis of the silver staining gel image of 2-DE for protein spots segmentation2010

    • Author(s)
      Michimasa Kishimoto
    • Journal Title

      Journal of Electophoresis 51

      Pages: 1-7

    • NAID

      130000254316

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Screening of PC and PMMA-binding peptides for site-specific immobilization of protein

    • Author(s)
      Y. Kumada, S. Murata, Y. Ishikawa, K. Nakatsuka, M. Kishimoto
    • Journal Title

      Journal of Biotechnology

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteome analysis of threonine-limited lysine fermentation by Brevi-bacterium flavum using two-dimentional electrophoresis

    • Author(s)
      S.Kasuya, K.Tada, T.Kanno, M.Kishimoto, J.Horiuchi
    • Journal Title

      Journal of Chemical Engineering of Japan

      Volume: (掲載確定)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Analysis of Fermentation Process for Scfv Production by Proteomics2011

    • Author(s)
      Y. Fujiki, Y. Kumada, M. Kishimoto
    • Organizer
      AIChE Annual Meeting Minnneapolis in USA
    • Year and Date
      2011-10-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of Fermentation Process for Scfv by Proteomics2011

    • Author(s)
      Y.Fujiki, Y.Kumada, M.Kishimoto
    • Organizer
      2011 AIChE(米国化学工学会)Annual Meeting
    • Place of Presentation
      Convention center,ミネアポリスUSA
    • Year and Date
      2011-10-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] プロテオーム解析をもちいた半導体センサ親和性ペプチドのスクリーニング2011

    • Author(s)
      大塚武、寺田貴裕、岸本通雅、熊田陽一
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Related Report
      2011 Final Research Report
  • [Presentation] タンパク質固定化用プラスチック親和性ペプチドの評価2011

    • Author(s)
      石川泰行、村田祥、熊田陽一、岸本通雅
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Related Report
      2011 Final Research Report
  • [Presentation] プロテオーム解析を用いた半導体センサー親和性ペプチドのスクリーニング2011

    • Author(s)
      大塚武、寺田貴裕、岸本通雅、熊田陽一
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] プロテオーム解析による抗体生産培養の検証2011

    • Author(s)
      岸本通雅、藤木裕也、熊田陽一、堀内淳一
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] 育種および培養プロセスへのバイオ情報の役割について2010

    • Author(s)
      岸本通雅
    • Organizer
      化学工学会 第42回秋季大会
    • Place of Presentation
      同志社大学(京都)
    • Year and Date
      2010-09-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] プロテオーム解析技術を用いた親和性ペプチドの探索および評価2010

    • Author(s)
      大塚武
    • Organizer
      化学工学会 第42回秋季大会
    • Place of Presentation
      同志社大学(京都)
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] ポリスチレン基盤への低分子抗体の部位特異的固定化と活性発現2009

    • Author(s)
      熊田陽一
    • Organizer
      化学工学会秋季大会
    • Place of Presentation
      広島大学
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] ポリカーボネート及び/またはポリメタックリル酸メチル親和性ぺプチド、およびその利用2010

    • Inventor(s)
      熊田陽一、岸本通雅、中塚一喜、村田祥
    • Industrial Property Rights Holder
      京都工芸繊維大学
    • Industrial Property Number
      2010-003166
    • Filing Date
      2010-02-16
    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi