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Development of Fluorescent Probes for the Detection of Various Angiogenesis Factors and Their Application to Medical Diagnostics

Research Project

Project/Area Number 21560814
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

SUZUKI Yoshio  独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (60321907)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsバイオセンサー / 血管内皮細胞増殖因子 / 蛍光 / タンパク質 / センサー / 分子プローブ / 色素
Research Abstract

Design and synthesis of fluorescent probes for the detection of angiogenesis was carried out based on the knowledge obtained by the development of fluorescent reagents for the detection of proteins. As a result, molecular probes for the detection of VEGF constructed by peptides and fluorescent molecules were developed, and the highly sensitive and easy detection of VEGF was successful with the use of nano-pillar substrates.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (37 results)

All 2012 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (23 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Development of Functional Fluorescent Molecular Probes and Their Biological Applications2011

    • Author(s)
      鈴木祥夫、横山憲二
    • Journal Title

      Encyclopedia of Nanoscience and Nanotechnology

      Volume: Vol.18 Pages: 1-20

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a more sensitive fluorescence sensing material for the detection of vascularendothelial growth factor, a biomarker for angiogenesis, prepared by combining a fluorescent peptide and a nanopillar substrate2011

    • Author(s)
      鈴木祥夫、横山憲二
    • Journal Title

      Biosensors and Bioelectronics

      Volume: Vol.26 Issue: 8 Pages: 3696-3699

    • DOI

      10.1016/j.bios.2011.02.007

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Design and Synthesis of New Fluorescent Probe for Rapid and Highly Sensitive Detection of Proteins via Electrophoretic Gel Stain2011

    • Author(s)
      鈴木祥夫、高木信幸、千室智之、篠原淳、坂口菜央、平塚淳典、横山憲二
    • Journal Title

      Electrophoresis

      Volume: Vol.32 Issue: 12 Pages: 1403-1413

    • DOI

      10.1002/elps.201000691

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 血管内皮細胞増殖因子(VEGF)を検出するための蛍光ペプチドの創製2011

    • Author(s)
      鈴木祥夫、横山憲二
    • Journal Title

      細胞

      Volume: Vol.43 Pages: 32-33

    • NAID

      130006999561

    • Related Report
      2011 Final Research Report
  • [Journal Article] Development of Functional Fluorescent Molecular Probes and Their Biological Applications2011

    • Author(s)
      鈴木祥夫
    • Journal Title

      Encyclopedia of Nanoscience and Nanotechnology

      Volume: 13 Pages: 1-20

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 血管内皮細胞増殖因子(VEGF)を検出するための蛍光ペプチドの創製2011

    • Author(s)
      鈴木祥夫
    • Journal Title

      細胞

      Volume: 43 Pages: 32-33

    • NAID

      130006999561

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Development of Functional Fluorescent Molecular Probes and Their Biological Applications2011

    • Author(s)
      Yoshio Suzuki, Kenji Yokoyama
    • Journal Title

      Encyclopedia of Nanoscience and Nanotechnology

      Volume: (掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a more sensitive fluorescence sensing material for the detection of vascularendothelial growth factor, a biomarker for angiogenesis, prepared by combining a fluorescent peptide and a nanopillar substrate2011

    • Author(s)
      Yoshio Suzuki, Kenji Yokoyama
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 26(掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design and Synthesis of New Fluorescent Probe for Rapid and Highly Sensitive Detection of Proteins via Electrophoretic Gel Stain2011

    • Author(s)
      Yoshio Suzuki, Nobuyuki Takagi, Tomoyuki Chimuro, Atsushi Shinohara, Nao Sakaguchi, Atsunori Hiratsuka, Kenji Yokoyama
    • Journal Title

      Electrophoresis

      Volume: 32(掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Fluorescent Peptide for the Detection of Vascular Endothelial Growth Factor(VEGF)2009

    • Author(s)
      鈴木祥夫、横山憲二
    • Journal Title

      ChemBioChem

      Volume: Vol.10 Issue: 11 Pages: 1793-1795

    • DOI

      10.1002/cbic.200900190

    • NAID

      130006999561

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Fluorescent Peptide for the Detection of Vascular Endothelial Growth Factor(VEGF)2009

    • Author(s)
      Yoshio Suzuki, Kenji Yokoyama
    • Journal Title

      ChemBioChem 10

      Pages: 1793-1795

    • NAID

      130006999561

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel fluorescent probe for analysis of hydroperoxides based on boron dipyrromethane fluorophore2009

    • Author(s)
      Naoko Inoue, Yoshio Suzuki, Kenji Yokoyama, Isao Karube
    • Journal Title

      BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 73

      Pages: 1215-1217

    • NAID

      10027541610

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] タンパク質を分光光学的に検出するための機能性材料の創製、「ナノ構造・物性」2012

    • Author(s)
      鈴木祥夫
    • Organizer
      第四回研究会
    • Place of Presentation
      神戸
    • Year and Date
      2012-01-20
    • Related Report
      2011 Final Research Report
  • [Presentation] タンパク質を分光光学的に検出するための機能性材料の創製2012

    • Author(s)
      鈴木祥夫
    • Organizer
      「ナノ構造・物性」第四回研究会
    • Place of Presentation
      兵庫県神戸大学アカデミア館(招待講演)
    • Year and Date
      2012-01-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] メロシアニン構造を有する新規蛍光分子プローブを利用したタンパク質の高感度分析2011

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] メロシアニン構造を有する新規蛍光分子プローブを利用したタンパク質の高感度分析2011

    • Author(s)
      鈴木祥夫
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      愛知県名古屋大学
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新規タンパク質検出用蛍光分子プローブの創製とプロテオーム解析への応用2011

    • Author(s)
      鈴木祥夫
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      茨城県つくば国際会議場
    • Year and Date
      2011-09-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Novel Fluorescent Reagents for High-Throughput and Highly-Sensitive In-Gel Protein Detection and Their Application to Proteomic Research2011

    • Author(s)
      鈴木祥夫、高木信幸、千室智之、篠原淳、坂口菜央、横山憲二
    • Organizer
      HUPO2011
    • Place of Presentation
      スイス
    • Year and Date
      2011-09-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Novel Fluorescent Reagents for High-Throughput and Highly-Sensitive In-Gel Protein Detection and Their Application to Proteomic Research2011

    • Author(s)
      鈴木祥夫
    • Organizer
      HUPO2011
    • Place of Presentation
      スイス(ジュネーブ)PALEXPO
    • Year and Date
      2011-09-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] メロシアニン骨格を有する新規タンパク質検出用蛍光分子プローブの創製とSDS-PAGEへの応用2011

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      日本プロテオーム学会2011年大会
    • Place of Presentation
      新潟
    • Year and Date
      2011-07-28
    • Related Report
      2011 Final Research Report
  • [Presentation] メロシアニン骨格を有する新規タンパク質検出用蛍光分子プローブの創製とSDS-PAGEへの応用2011

    • Author(s)
      鈴木祥夫
    • Organizer
      日本プロテオーム学会2011年大会
    • Place of Presentation
      新潟県朱鷺メッセ
    • Year and Date
      2011-07-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] メロシアニン骨格を有する新規タンパク質検出用蛍光分子プローブの創製2011

    • Author(s)
      鈴木祥夫
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      大阪府ホテル阪急エキスポパーク
    • Year and Date
      2011-06-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of Fluorescent Reagents for the Detection of Proteins and Their Application to High-throughput Protein Analysis2010

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      Pacifichem2010
    • Place of Presentation
      ハワイ
    • Year and Date
      2010-12-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of Fluorescent Reagents for the Detection of Proteins and Their Application to High-throughput Protein Analysis2010

    • Author(s)
      Yoshio Suzuki, Kenji Yokoyama
    • Organizer
      Pacifichem 2010
    • Place of Presentation
      ハワイコンベンションセンター(アメリカ)
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 血管内皮細胞増殖因子(VEGF)を検出するための蛍光ペプチドの創製2010

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      第4回バイオ関連化学シンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 血管内皮細胞増殖因子(VEGF)を検出するための蛍光ペプチドの創製2010

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      第4回バイオ関連化学シンポジウム
    • Place of Presentation
      大阪大学豊中キャンパス(大阪府)
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 血管内皮細胞増殖因子(VEGF)検出用蛍光分子プローブの創製とナノピラーアレイ上における検出2010

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 血管内皮細胞増殖因子(VEGF)検出用蛍光分子プローブの創製とナノピラーアレイ上における検出2010

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学川内北キャンパス(宮城県)
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新規タンパク質検出用蛍光分子プローブの創製とSDS-PAGEにおける簡易染色法への応用2010

    • Author(s)
      鈴木祥夫、坂口菜央、平塚淳典、高木信幸、千室智之、篠原淳、横山憲二
    • Organizer
      日本プロテオーム学会2010年会
    • Place of Presentation
      東京ベイホテル東急(千葉県)
    • Year and Date
      2010-07-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新規タンパク質検出用蛍光分子プローブの創製2010

    • Author(s)
      鈴木祥夫、高木信幸、千室智之、篠原淳、坂口菜央、平塚淳典、横山憲二
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2010-06-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 血管内皮細胞増殖因子(VEGF)を検出するための機能性ペプチドの創製2010

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      第4回ナノバイオデバイス・ワークショップ
    • Place of Presentation
      産総研つくばセンター
    • Year and Date
      2010-01-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規タンパク質検出用蛍光分子プローブの創製とSDS-PAGEへの応用2009

    • Author(s)
      鈴木祥夫、坂口菜央、平塚淳典、高木信幸、千室智之、篠原淳、横山憲二
    • Organizer
      日本分析化学会第58年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規タンパク質検出用蛍光分子プローブの創製とSDS-PAGEにおける簡易染色法への応用2009

    • Author(s)
      鈴木祥夫、坂口菜央、平塚淳典、高木信幸、千室智之、篠原淳、横山憲二
    • Organizer
      第24回生体機能関連化学シンポジウム,第12回バイオテクノロジー部会シンポジウム
    • Place of Presentation
      九州大学医系キャンパス・百年記念講堂
    • Year and Date
      2009-09-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] 血管内皮細胞増殖因子(VEGF)を検出するための蛍光ペプチドの創製2009

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      日本ヒトプロテオーム機構(JHUPO)第7回大会
    • Place of Presentation
      北里大学薬学部
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規タンパク質検出用分子プローブの創製2009

    • Author(s)
      鈴木祥夫、横山憲二
    • Organizer
      第9回 日本蛋白質科学会年会
    • Place of Presentation
      熊本全日空ホテルニュースカイ
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] タンパク質を検出するための分析試薬2010

    • Inventor(s)
      鈴木祥夫、横山憲二
    • Industrial Property Rights Holder
      (独)産業技術総合研究所
    • Industrial Property Number
      2010-170805
    • Filing Date
      2010-07-29
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Patent(Industrial Property Rights)] タンパク質の分析方法および分析試薬2009

    • Inventor(s)
      鈴木祥夫、横山憲二、高木信幸、千室智之、篠原淳
    • Industrial Property Rights Holder
      (独)産業技術総合研究所
    • Filing Date
      2009-06-26
    • Related Report
      2009 Annual Research Report
    • Overseas

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Published: 2009-04-01   Modified: 2016-04-21  

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