Silencing mechanisms of plant heterochromatin regions

• Project/Area Number: 21570008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: HABU Yoshiki 独立行政法人農業生物資源研究所, ゲノム機能改変研究ユニット, 主任研究員 (80266915)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: サイレンシング / 反復配列 / シトシンメチル化 / シロイヌナズナ / MORPHEUS' MOLECULE1 / エピジェネティクス / DNAメチル化 / ヒストンメチル化 / Morpheus' Molecule 1 / トランスポゾン / 小分子RNA / Morpheus'Molecule 1

Research Abstract

We analyzed function of factors involved in silencing of endogenous repetitive elements in the plant genome. The results suggested the presence of three independent silencing pathways regulated by(1) MORPHEUS' MOLECULE1(MOM1),(2) RNA-directed DNA methylation(RdDM), and(3) both of MOM1 and RdDM, respectively. Chromodomain is often found in MOM1 protein of plants other than Arabidopsis thaliana, but our analysis indicated that it is dispensable for silencing activity of MOM1.

Research Products

• [Journal Article] Tandemly arranged chalcone synthase A genes contribute to the spatially regulated expression of siRNA and the natural bicolor phenotype in Petunia hybrida (2012)
  • Author(s): Morita Y, Saito R, Ban Y, Tanikawa N, Kuchitsu K, Ando T, Yoshikawa Ma, Habu Y, Ozeki Y, Nakayama M
  • Journal Title: Plant J Volume: 70 Pages: 739-749
  • Peer Reviewed
• [Journal Article] siRNA-mediated chromatin maintenance and its function in Arabidopsis thaliana (2011)
  • Author(s): Kanno T, Habu Y
  • Journal Title: Biochim Biophys Acta-Gene Regulatory Mechanisms Volume: 1809 Pages: 444-451
  • Peer Reviewed
• [Journal Article] 植物におけるRNAサイレンシング機構:転写後型ジーンサイレンシング(PTGS)と転写型ジーンサイレンシング(TGS) (2011)
  • Author(s): 吉川学,菅野達夫,土生芳樹
  • Journal Title: 細胞工学 Pages: 706-711
• [Journal Article] siRNA-mediated chromatin maintenance and its function in Arabidopsis thaliana (2011)
  • Author(s): Tatsuo Kanno, Yoshki Habu
  • Journal Title: Biochim Biophys Acta Volume: 1809 Issue: 8 Pages: 444-451
  • DOI: 10.1016/j.bbagrm.2011.05.002
  • Peer Reviewed
• [Journal Article] Transduction of RNA-directed DNA methylation signals to repressive histone marks in Arabidopsis thaliana (2010)
  • Author(s): Numa H, Kim J-M, Matsui A, Kurihara Y, Morosawa T, Ishida J, Mochizuki Y, Kimura H, Shinozaki K, Toyoda T, Seki M, Yoshikawa M, Habu Y
  • Journal Title: EMBO J Volume: 29 Pages: 352-362
  • Peer Reviewed
• [Journal Article] Locus-specific dependency of endogenous silent loci on MOM1 and non-CG methylation in Arabidopsis thaliana (2010)
  • Author(s): Habu Y, Yoshikawa M
  • Journal Title: Plant Signal Behav Volume: 5 Pages: 724-726
  • Peer Reviewed
• [Journal Article] Epigenetic silencing of endogenous repetitive sequences by MORPHEUS' MOLECULE1 in Arabidopsis thaliana (2010)
  • Author(s): Habu Y
  • Journal Title: Epigenetics Volume: 5 Pages: 562-565
• [Journal Article] Locus-specific dependency of endogenous silent loci on MOM1 and non-CG methylation in Arabidopsis thaliana. (2010)
  • Author(s): 土生芳樹, 他
  • Journal Title: Plant Signaling & Behavior Volume: 5 Pages: 724-726
• [Journal Article] A phenomics approach detected differential epigenetic growth regulation between inbreds and their hybrid in Oryza sativa. (2010)
  • Author(s): 七夕高也, 他
  • Journal Title: Molecular Breeding Volume: 26 Pages: 729-734
  • Peer Reviewed
• [Journal Article] Epigenetic silencing of endogenous repetitive sequences by MORPHEUS' MOLECULE1 in Arabidopsis thaliana. (2010)
  • Author(s): 土生芳樹
  • Journal Title: Epigenetics Volume: 5 Pages: 562-565
• [Journal Article] Transduction of RNA-directed DNA methylation signals to repressive histone marks in Arabidopsis thaliana (2010)
  • Author(s): 沼寿隆, 他
  • Journal Title: The EMBO Journal VOL.29 Pages: 352-362
  • Peer Reviewed
• [Presentation] ペチュニア園芸品種(Petunia x hybrida)にピコティ形質を付与する低分子RNAの解析 (2011)
  • Author(s): 森田裕将, 他
  • Organizer: 園芸学会平成23年度春季大会
  • Place of Presentation: 宇都宮
  • Year and Date: 2011-03-20
• [Presentation] クロマチン修飾を介した植物のゲノム組成改変 (2011)
  • Author(s): 土生芳樹
  • Organizer: 第17回植物バイテクシンポジウム「エピゲノム研究の展開と植物科学」
  • Place of Presentation: 京都府立大学
  • Year and Date: 2011-01-27
• [Presentation] クロマチン修飾を介した植物のゲノム組成改変 (2011)
  • Author(s): 土生芳樹
  • Organizer: 第17回京都府立大学植物バイテクシンポジウム「エピゲノム研究の展開と植物科学」
• [Presentation] Processing of miR173-targeted TAS RNAs in Arabidopsis thaliana (2011)
  • Author(s): Yoshikawa M, 他
  • Organizer: The 16th Annual Meeting of the RNA Society and The 13th Annual Meeting of the RNA Society of Japan
  • Place of Presentation: 京都
• [Presentation] Involvement of SGS3 in trans-acting siRNA production in Arabidopsis thaliana (2011)
  • Author(s): Yoshikawa M, 他
  • Organizer: NTU-JST Join Meeting on RNA and Biofunctions-Asia Studies
  • Place of Presentation: 台北(台湾)
• [Presentation] シロイヌナズナのサイレンシング因子Morpheus' Molecule 1 (MOM1)による内在性領域不活性化機構の解析 (2009)
  • Author(s): 土生芳樹, 他
  • Organizer: 日本遺伝学会第81回大会
  • Place of Presentation: 信州大学理学部
  • Year and Date: 2009-09-16
• [Presentation] 育種におけるクロマチン修飾:減数分裂期組み換えとのかかわり日本育種学会シンポジウム「RNAサイレンシング研究の新展開とその育種的利用に向けた展望」 (2009)
  • Author(s): 土生芳樹
  • Organizer: 日本育種学会2009年秋季大会
• [Presentation] ゲノムタイリングアレイが拓くヘテロクロマチンサイレンシングの新局面日本植物生理学会シンポジウム「植物科学におけるタイリングアレイと次世代シーケンサーの利用」 (2009)
  • Author(s): 土生芳樹
  • Organizer: 第50回日本植物生理学会年会
• [Book] Mechanisms linking cytosine methylation to histone modification in Arabidopsis thaliana. In : Noncoding RNAs in plants (2011)
  • Author(s): Arita K, Kanno T, Yoshikawa M, Habu Y
  • Publisher: Springer
• [Book] Mechanisms linking cytosine methylation to histone modification in Arabidopsis thaliana. In : Non coding RNAs in plants (2011)
  • Author(s): Kyohei Arita, et al
  • Publisher: Springer
• [Book] 植物におけるRNAサイレンシング機構:転写後型ジーンサイレンシング(PTGS)と転写型ジーンサイレンシング(TGS) (2011)
  • Author(s): 吉川学, 他
  • Publisher: 学研メディカル秀潤社
• [Remarks]
  • URL: http://www.nias.affrc.go.jp/press/20100121/
• [Remarks]
  • URL: http://www.nias.affrc.go.jp/press/20100121/