| Project/Area Number |
21570050
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SASAKI Tadamasa 東京理科大学, 基礎工学部・生物工学科, 助教 (50432802)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 植物分子機能 / イネ / 種子 / 転写因子 / 貯蔵物質 / 生合成 / 生産制御 / 転写制御因子 / 貯蔵物質生合成 / 種子形成 / タンパク質相互作用 / 登熟期 / 遺伝子発現制御 / ATP合成 |
|---|
| Research Abstract |
An endosperm mutant flo2 is represented by reduced production of seed storage starch and storage proteins, and results in small chalky gains. This suggests that the gene responsible to this mutation encodes a regulatory gene, which may globally control the biosynthesis of storage substances. We isolated the gene responsible to the flo2 mutation by a map-based cloning method. This gene, named FLO2, contained a TPR motif, in which a point mutation occurred in the mutant gene. The wild-type FLO2 gene completely restored the phenotype of the flo2 mutation, indicating that this gene was the gene responsible to the flo2 mutation. The expression of FLO2 gene was detected in leaves and immature seeds. We found that the FLO2 protein interacted with a bHLH transcription factor and a LEA protein, suggesting that this protein may be involved in regulation of the gene expression for production of the storage substances. Overexpression of the FLO2 gene indicated overexpression of the genes for storage proteins and storage starch, resulting in enlargement of the grain size.
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