Elucidation of flavone C-glycosides biosynthesis pathway in rice based on transcriptome analysis

• Project/Area Number: 21570057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SAKAKIBARA Keiko 独立行政法人理化学研究所, メタボローム機能研究グループ, 上級研究員 (20360555)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: C-配糖化 / フラボノイド / トランスクリプトミクス / 二次代謝 / イネ / 植物 / 酵素 / 配糖化酵素

Research Abstract

Transcriptome coexpression analysis based on correlation coefficients database developed independently showed that the expression patterns of genes in flavonoid C-glycoside biosynthetic pathway are correlated with genes encoding chalcone synthase and chalcone isomerase and suggests some genes as candidates involved in flavonoid C-glycoside biosynthesis. By flavonoid analyses using several rice organs, 29 flavonoid-related peaks were detected and the structures of 11 peaks were supposed. Additional flavonoid related peaks were detected in some rice FOX lines. The functions of candidate genes were assayed by measurement of enzymatic activity using recombinant proteins and overexpression in Arabidopsis in addition to molecular genetic analysis.

Research Products

• [Journal Article] An evolutionary view of functional diversity in family 1 glycosyltransferases (2011)
  • Author(s): K. Yonekura-Sakakibara, K. Hanada
  • Journal Title: Plant J. Volume: 66 Pages: 182-193
  • NAID: 130006996981
  • Peer Reviewed
• [Journal Article] An evolutionary view of functional diversity in family 1 glycosyltrans ferases (2011)
  • Author(s): K.Yonekura-Sakakibara, K.Hanada
  • Journal Title: The Plant Journal Volume: 66 Issue: 1 Pages: 182-193
  • DOI: 10.1111/j.1365-313x.2011.04493.x
  • Peer Reviewed
• [Journal Article] An evolutionary view of functional diversity in family 1 glycosyltransferases (2011)
  • Author(s): Keiko Yonekura-Sakakibara
  • Journal Title: The Plant Journal Volume: (印刷中)
  • NAID: 130006996981
  • Peer Reviewed
• [Presentation] シロイヌナズナ・ファミリー1配糖化酵素の機能ゲノム学 (2011)
  • Author(s): 榊原圭子
  • Organizer: P450 &UGT,SULT勉強会2011
  • Place of Presentation: 大阪大学吹田キャンパス(大阪府吹田市)(招待講演)
  • Year and Date: 2011-06-18
• [Presentation] ファミリー1配糖化酵素の機能多様性における進化的考察 (2011)
  • Author(s): 榊原圭子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 日本、仙台
  • Year and Date: 2011-03-21
• [Presentation] シロイヌナズナ・ファミリー1配糖化酵素の機能ゲノム学 (2011)
  • Author(s): 榊原圭子
  • Organizer: P450, SULT, UGT勉強会
  • Place of Presentation: 大阪・吹田
• [Presentation] ファミリー1配糖化酵素の機能多様性における進化的考察 (2011)
  • Author(s): 榊原圭子、花田耕介、斉藤和季
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 仙台
• [Book] 遺伝子発現様式に基づく遺伝子の単離と機能同定植物の分子育種学 (2011)
  • Author(s): 榊原圭子
  • Publisher: 講談社サイエンティフィック