Elucidation of flavone C-glycosides biosynthesis pathway in rice based on transcriptome analysis
| Project/Area Number |
21570057
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SAKAKIBARA Keiko 独立行政法人理化学研究所, メタボローム機能研究グループ, 上級研究員 (20360555)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | C-配糖化 / フラボノイド / トランスクリプトミクス / 二次代謝 / イネ / 植物 / 酵素 / 配糖化酵素 |
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| Research Abstract |
Transcriptome coexpression analysis based on correlation coefficients database developed independently showed that the expression patterns of genes in flavonoid C-glycoside biosynthetic pathway are correlated with genes encoding chalcone synthase and chalcone isomerase and suggests some genes as candidates involved in flavonoid C-glycoside biosynthesis. By flavonoid analyses using several rice organs, 29 flavonoid-related peaks were detected and the structures of 11 peaks were supposed. Additional flavonoid related peaks were detected in some rice FOX lines. The functions of candidate genes were assayed by measurement of enzymatic activity using recombinant proteins and overexpression in Arabidopsis in addition to molecular genetic analysis.
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Report
(4 results)
Research Products
(8 results)
