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Molecular mechanisms of sexual plasticity during testicular differentiation and development in the medaka, Olyzias latipes

Research Project

Project/Area Number 21570065
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionUniversity of Shizuoka

Principal Investigator

KOBAYASHI Tohru  静岡県立大学, 環境科学研究所, 教授 (30221972)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsメダカ / 性的可塑性 / 性分化 / 精巣卵 / エストロゲン / dmrt1 / foxl2 / セルトリ細胞 / dmrtl / 性転換 / 精巣
Research Abstract

This study focuses on the molecular mechanisms of sexual plasticity of testicular somatic cells during testis differentiation and development in the medaka, Olyzias latipes. In XY medaka of Hd-rR strain, estrogen treatment induces the female differentiation when estrogen expose to the fry before 30 dph(days post hatching). During XY sex reversal by estrogen, Sertoli cells showed the expression of foxl2, whereas the expression of male differentiation maker genes of germ-cell supporting cells were decreased and subsequently suppressed, such as gsdf and dmrt1 etc. Although estrogen induced testis-ova differentiation easily in mature fish, XY sex reversal(ovarian differentiation) was not induced. In mature XY fish treated by estrogen, foxl2 expression was induced. However, expression of male differentiation maker genes was not suppressed completely. These results suggest strongly that sexual plasticity of Sertoli cells is limited with the progression of testis differentiation in medaka.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (25 results)

All 2012 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 7 results) Presentation (12 results) Book (1 results)

  • [Journal Article] Expression of 3υ-hydroxysteroid dehydrogenase(hsd3b), star and ad4bp/sf-1 during gonadal development in medaka(Oryzias latipes)2012

    • Author(s)
      Nakamoto, M., Fukasawa, M., Tanaka, S., Shimamori, K., Suzuki, A., Matsuda, M., Kobayashi, T., Nagahama, Y. and Shibata, N.
    • Journal Title

      Gen. Comp. Endocrinol

      Volume: 176 Pages: 222-230

    • Related Report
      2011 Final Research Report
  • [Journal Article] Comparative Responsiveness to Natural and Synthetic Estrogens of Fish species Commonly Used in the Laboratory and Field Monitoring2012

    • Author(s)
      Lange, A., Kobayashi, T., et al
    • Journal Title

      Aquatic Toxicology

      Volume: 109 Pages: 250-258

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol2012

    • Author(s)
      Hirakawa, I., Kobayashi, T., et al
    • Journal Title

      Chemosphere

      Volume: 87 Pages: 668-674

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of 3β-hydroxysteroid dehydrogenase (hsd3b), star and ad4bp/sf-1 during gonadal development in medaka (Oryzias latipes)2012

    • Author(s)
      Nakamoto, M., Kobayashi, T., et al
    • Journal Title

      General and Comparative Endocrinology

      Volume: 176 Pages: 222-230

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative Responsiveness to Natural and Synthetic Estrogens of Fish Species Commonly Used in the Laboratory and Field Monitoring2011

    • Author(s)
      Lange, A., Katsu, Y., Miyagawa, S., Ogino, Y., Urushutani, H., Kobayashi, T., Hirai, T., Shears, J. A., Nagae, M., Yamamoto, J., Ohnishi, Y., Oka, T., Tatarazako, N., Ohta, Y., Tyler, C. R. and Iguchi, T.
    • Journal Title

      Aquatic Toxicology

      Volume: 109 Pages: 250-258

    • Related Report
      2011 Final Research Report
  • [Journal Article] Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka(Oryzias latipes) exposed to 17τ-ethinylestradiol2011

    • Author(s)
      Hirakawa, I., Miyagawa, S., Katsu, Y., Kagami, Y., Tatarazako, N., Kobayashi, T., Kusano, T., Ogino, Y., Ohta, Y. and Iguchi, T.
    • Journal Title

      Chemosphere

      Volume: 87 Pages: 668-674

    • Related Report
      2011 Final Research Report
  • [Journal Article] In vitro germ cell differentiation during sex differentiation in a teleost fish2010

    • Author(s)
      Kobayashi, T.
    • Journal Title

      Int. J. Dev. Biol

      Volume: 54 Pages: 105-111

    • Related Report
      2011 Final Research Report
  • [Journal Article] The cloning and expression of medaka cholesterol side chain cleavage cytochrome P450(P450scc) during gonadal development2010

    • Author(s)
      Nakamoto, M., Fukasawa, M., Orii, S., Shimamori, K., Maeda, T., Suzuki, A., Matsuda, M., Kobayashi, T., Nagahama, Y. and Shibata, N.
    • Journal Title

      Develop. Growth and Differ

      Volume: 52 Pages: 385-395

    • Related Report
      2011 Final Research Report
  • [Journal Article] Cloning and expression of medaka cholesterol side chain cleavage cytochrome P450 during gonadal development2010

    • Author(s)
      Nakamoto M., Kobayashi, T., et al.
    • Journal Title

      Development, Growth and Differentiation

      Volume: 52 Pages: 385-395

    • NAID

      10027564905

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Doublesex- and mab-3-related transcription factor-1 repression of aromatase transcription, apossible mechanism favoring the male pathway in tilapia2010

    • Author(s)
      Wang, DS., Kobayashi, T., et al.
    • Journal Title

      Endocrinology

      Volume: 151 Pages: 1331-1340

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro germ cell differentiation during sex differentiation in a teleost fish2010

    • Author(s)
      Kobayashi, T.
    • Journal Title

      International Journal of Developmental Biology 54

      Pages: 105-111

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular aspects of gonadal differentiation in a teleost fish, Nile tilapia2009

    • Author(s)
      Kobayashi, T., Nagahama, Y.
    • Journal Title

      Sexual Development 3

      Pages: 108-117

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Sexual dimorphisms in somatic cells in the testis of medaka during estrogen-induced testis-ova differentiation.2012

    • Author(s)
      Kobayashi, T.
    • Organizer
      Sixth International symposium on the Biology of Vertebrate Sex Determination
    • Place of Presentation
      Kona, Hawaii
    • Related Report
      2011 Final Research Report
  • [Presentation] 魚類の生殖腺性分化と性的可塑性2011

    • Author(s)
      小林亨
    • Organizer
      シンポジウム「沖縄における水棲動物生殖の基礎と応用」(財団法人海洋博記念公園管理財団及び琉球大学熱帯生物圏研究センター)
    • Place of Presentation
      財団法人海洋博記念公園管理財団(沖縄)
    • Year and Date
      2011-12-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Comparative Responsiveness to test and sentinel fish species to natural and synthetic oestrogens2011

    • Author(s)
      Lange, A., Katsu, Y., Miyagawa, S., Ogino, Y., Urushutani, H., Kobayashi, T., Hirai, T., Shears, J. A., Nagae, M., Yamamoto, 5 J., Ohnishi, Y., Oka, T., Tatarazako, N., Ohta, Y., Tyler, C. R. and Iguchi, T.
    • Organizer
      9th International Symposium on Reproductive Physiology of Fish
    • Place of Presentation
      India
    • Related Report
      2011 Final Research Report
  • [Presentation] 魚類の生殖腺性分化と性的可塑性2011

    • Author(s)
      小林亨
    • Organizer
      海洋博覧会記念公園管理財団・琉球大学熱帯生物圏研究センター共催のシンポジウム:沖縄における水棲動物生殖の基礎と応用
    • Related Report
      2011 Final Research Report
  • [Presentation] メダカ精巣卵誘起時における遺伝子発現解析2011

    • Author(s)
      宮川信一・平川郁美・勝義直・小林亨・井口泰泉
    • Organizer
      第83回日本動物学会大会
    • Place of Presentation
      旭川
    • Related Report
      2011 Final Research Report
  • [Presentation] エストロゲンによるメダカ精巣卵形成誘起過程における精原細胞の動態2011

    • Author(s)
      千葉文香・佐藤忠・井口泰泉・濱口哲・小林亨
    • Organizer
      第36回日本比較内分泌学会大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 魚類性分化過程における組織構築と性的可塑性2010

    • Author(s)
      小林亨
    • Organizer
      日本動物学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 魚類性分化過程における組織構築と性的可塑性2010

    • Author(s)
      小林亨・堀江好文・千葉文香
    • Organizer
      第82回日本動物学会大会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Histology and gene expression analysis in testis of medaka exposed by ethinylestradiol2010

    • Author(s)
      Hirakawa, H., Kagami, Y., Tatarazako, N., Kobayashi, T., Kusano, T., Ogino, Y., Ohta, Y., Miyagawa, S., Iguchi, T.
    • Organizer
      環境ホルモン学会第13回研究発表会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 魚類精巣分化過程にお希有r体細胞の挙動2009

    • Author(s)
      小林亨
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡コンベンショナルアーツセンター「グランシップ」
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Testicular differentiation in teleosts : Formation of seminiferous tubule structure2009

    • Author(s)
      Kobayashi, T.
    • Organizer
      Fifth International Symposium on the Biology of Vertebrate Sex Determination
    • Place of Presentation
      Kona, Hawaii
    • Related Report
      2011 Final Research Report
  • [Presentation] 魚類性分化過程における体細胞の動態2009

    • Author(s)
      小林亨
    • Organizer
      第81回日本動物学会大会
    • Place of Presentation
      静岡
    • Related Report
      2011 Final Research Report
  • [Book] 「性は面白い」静岡県立大学公開講座要旨集2010

    • Author(s)
      小林亨
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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