Molecular mechanisms of sexual plasticity during testicular differentiation and development in the medaka, Olyzias latipes

• Project/Area Number: 21570065
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: University of Shizuoka
• Principal Investigator: KOBAYASHI Tohru 静岡県立大学, 環境科学研究所, 教授 (30221972)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: メダカ / 性的可塑性 / 性分化 / 精巣卵 / エストロゲン / dmrt1 / foxl2 / セルトリ細胞 / dmrtl / 性転換 / 精巣

Research Abstract

This study focuses on the molecular mechanisms of sexual plasticity of testicular somatic cells during testis differentiation and development in the medaka, Olyzias latipes. In XY medaka of Hd-rR strain, estrogen treatment induces the female differentiation when estrogen expose to the fry before 30 dph(days post hatching). During XY sex reversal by estrogen, Sertoli cells showed the expression of foxl2, whereas the expression of male differentiation maker genes of germ-cell supporting cells were decreased and subsequently suppressed, such as gsdf and dmrt1 etc. Although estrogen induced testis-ova differentiation easily in mature fish, XY sex reversal(ovarian differentiation) was not induced. In mature XY fish treated by estrogen, foxl2 expression was induced. However, expression of male differentiation maker genes was not suppressed completely. These results suggest strongly that sexual plasticity of Sertoli cells is limited with the progression of testis differentiation in medaka.

Research Products

• [Journal Article] Expression of 3υ-hydroxysteroid dehydrogenase(hsd3b), star and ad4bp/sf-1 during gonadal development in medaka(Oryzias latipes) (2012)
  • Author(s): Nakamoto, M., Fukasawa, M., Tanaka, S., Shimamori, K., Suzuki, A., Matsuda, M., Kobayashi, T., Nagahama, Y. and Shibata, N.
  • Journal Title: Gen. Comp. Endocrinol Volume: 176 Pages: 222-230
• [Journal Article] Comparative Responsiveness to Natural and Synthetic Estrogens of Fish species Commonly Used in the Laboratory and Field Monitoring (2012)
  • Author(s): Lange, A., Kobayashi, T., et al
  • Journal Title: Aquatic Toxicology Volume: 109 Pages: 250-258
  • Peer Reviewed
• [Journal Article] Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka (Oryzias latipes) exposed to 17α-ethinylestradiol (2012)
  • Author(s): Hirakawa, I., Kobayashi, T., et al
  • Journal Title: Chemosphere Volume: 87 Pages: 668-674
  • Peer Reviewed
• [Journal Article] Expression of 3β-hydroxysteroid dehydrogenase (hsd3b), star and ad4bp/sf-1 during gonadal development in medaka (Oryzias latipes) (2012)
  • Author(s): Nakamoto, M., Kobayashi, T., et al
  • Journal Title: General and Comparative Endocrinology Volume: 176 Pages: 222-230
  • Peer Reviewed
• [Journal Article] Comparative Responsiveness to Natural and Synthetic Estrogens of Fish Species Commonly Used in the Laboratory and Field Monitoring (2011)
  • Author(s): Lange, A., Katsu, Y., Miyagawa, S., Ogino, Y., Urushutani, H., Kobayashi, T., Hirai, T., Shears, J. A., Nagae, M., Yamamoto, J., Ohnishi, Y., Oka, T., Tatarazako, N., Ohta, Y., Tyler, C. R. and Iguchi, T.
  • Journal Title: Aquatic Toxicology Volume: 109 Pages: 250-258
• [Journal Article] Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka(Oryzias latipes) exposed to 17τ-ethinylestradiol (2011)
  • Author(s): Hirakawa, I., Miyagawa, S., Katsu, Y., Kagami, Y., Tatarazako, N., Kobayashi, T., Kusano, T., Ogino, Y., Ohta, Y. and Iguchi, T.
  • Journal Title: Chemosphere Volume: 87 Pages: 668-674
• [Journal Article] In vitro germ cell differentiation during sex differentiation in a teleost fish (2010)
  • Author(s): Kobayashi, T.
  • Journal Title: Int. J. Dev. Biol Volume: 54 Pages: 105-111
• [Journal Article] The cloning and expression of medaka cholesterol side chain cleavage cytochrome P450(P450scc) during gonadal development (2010)
  • Author(s): Nakamoto, M., Fukasawa, M., Orii, S., Shimamori, K., Maeda, T., Suzuki, A., Matsuda, M., Kobayashi, T., Nagahama, Y. and Shibata, N.
  • Journal Title: Develop. Growth and Differ Volume: 52 Pages: 385-395
• [Journal Article] Cloning and expression of medaka cholesterol side chain cleavage cytochrome P450 during gonadal development (2010)
  • Author(s): Nakamoto M., Kobayashi, T., et al.
  • Journal Title: Development, Growth and Differentiation Volume: 52 Pages: 385-395
  • NAID: 10027564905
  • Peer Reviewed
• [Journal Article] Doublesex- and mab-3-related transcription factor-1 repression of aromatase transcription, apossible mechanism favoring the male pathway in tilapia (2010)
  • Author(s): Wang, DS., Kobayashi, T., et al.
  • Journal Title: Endocrinology Volume: 151 Pages: 1331-1340
  • Peer Reviewed
• [Journal Article] In vitro germ cell differentiation during sex differentiation in a teleost fish (2010)
  • Author(s): Kobayashi, T.
  • Journal Title: International Journal of Developmental Biology 54 Pages: 105-111
  • Peer Reviewed
• [Journal Article] Molecular aspects of gonadal differentiation in a teleost fish, Nile tilapia (2009)
  • Author(s): Kobayashi, T., Nagahama, Y.
  • Journal Title: Sexual Development 3 Pages: 108-117
  • Peer Reviewed
• [Presentation] Sexual dimorphisms in somatic cells in the testis of medaka during estrogen-induced testis-ova differentiation. (2012)
  • Author(s): Kobayashi, T.
  • Organizer: Sixth International symposium on the Biology of Vertebrate Sex Determination
  • Place of Presentation: Kona, Hawaii
• [Presentation] 魚類の生殖腺性分化と性的可塑性 (2011)
  • Author(s): 小林亨
  • Organizer: シンポジウム「沖縄における水棲動物生殖の基礎と応用」(財団法人海洋博記念公園管理財団及び琉球大学熱帯生物圏研究センター)
  • Place of Presentation: 財団法人海洋博記念公園管理財団(沖縄)
  • Year and Date: 2011-12-10
• [Presentation] Comparative Responsiveness to test and sentinel fish species to natural and synthetic oestrogens (2011)
  • Author(s): Lange, A., Katsu, Y., Miyagawa, S., Ogino, Y., Urushutani, H., Kobayashi, T., Hirai, T., Shears, J. A., Nagae, M., Yamamoto, 5 J., Ohnishi, Y., Oka, T., Tatarazako, N., Ohta, Y., Tyler, C. R. and Iguchi, T.
  • Organizer: 9th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: India
• [Presentation] 魚類の生殖腺性分化と性的可塑性 (2011)
  • Author(s): 小林亨
  • Organizer: 海洋博覧会記念公園管理財団・琉球大学熱帯生物圏研究センター共催のシンポジウム:沖縄における水棲動物生殖の基礎と応用
• [Presentation] メダカ精巣卵誘起時における遺伝子発現解析 (2011)
  • Author(s): 宮川信一・平川郁美・勝義直・小林亨・井口泰泉
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 旭川
• [Presentation] エストロゲンによるメダカ精巣卵形成誘起過程における精原細胞の動態 (2011)
  • Author(s): 千葉文香・佐藤忠・井口泰泉・濱口哲・小林亨
  • Organizer: 第36回日本比較内分泌学会大会
  • Place of Presentation: 東京
• [Presentation] 魚類性分化過程における組織構築と性的可塑性 (2010)
  • Author(s): 小林亨
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-23
• [Presentation] 魚類性分化過程における組織構築と性的可塑性 (2010)
  • Author(s): 小林亨・堀江好文・千葉文香
  • Organizer: 第82回日本動物学会大会
  • Place of Presentation: 東京
• [Presentation] Histology and gene expression analysis in testis of medaka exposed by ethinylestradiol (2010)
  • Author(s): Hirakawa, H., Kagami, Y., Tatarazako, N., Kobayashi, T., Kusano, T., Ogino, Y., Ohta, Y., Miyagawa, S., Iguchi, T.
  • Organizer: 環境ホルモン学会第13回研究発表会
  • Place of Presentation: 東京
• [Presentation] 魚類精巣分化過程にお希有r体細胞の挙動 (2009)
  • Author(s): 小林亨
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡コンベンショナルアーツセンター「グランシップ」
  • Year and Date: 2009-09-19
• [Presentation] Testicular differentiation in teleosts : Formation of seminiferous tubule structure (2009)
  • Author(s): Kobayashi, T.
  • Organizer: Fifth International Symposium on the Biology of Vertebrate Sex Determination
  • Place of Presentation: Kona, Hawaii
• [Presentation] 魚類性分化過程における体細胞の動態 (2009)
  • Author(s): 小林亨
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 静岡
• [Book] 「性は面白い」静岡県立大学公開講座要旨集 (2010)
  • Author(s): 小林亨