Elucidation of Activation Mechanism of Extracellular Matrix Protein in Related to Allergosis
| Project/Area Number |
21570123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
TAMADA Taro 独立行政法人日本原子力研究開発機構, 量子ビーム応用研究部門, 研究主幹 (50391248)
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| Co-Investigator(Kenkyū-buntansha) |
ADACHI Motoyasu 独立行政法人日本原子力研究開発機構, 子ビーム応用研究部門, 研究副主幹 (60293958)
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| Co-Investigator(Renkei-kenkyūsha) |
IZUHARA Kenji 佐賀大学, 医学部, 教授 (00270463)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ペリオスチン / アレルギー疾患 / 活性化機構 / アレルギー・ぜんそく / 蛋白質 / 放射線、X線、粒子線 |
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| Research Abstract |
Human periostin is an extracellular matrix protein in related to allergosis. We succeeded in preparation of functional region(R1-R4), including four sequential fasciclin 1 like domains, of human periostin using E. coli expression system combined with refolding method. Continuously, two anti-human periostin antibodies with sufficient binding ability to antigen were selected by gel filtration analysis, and then crystallization experiments using complex between R1-R4 and Fabs from these antibodies were carried out.
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Report
(4 results)
Research Products
(1 results)
