Analysis of the role of electrostatic interactions between cytoplasmic domains of sarcoplasmic reticulum calcium pump

• Project/Area Number: 21570127
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Asahikawa Medical College
• Principal Investigator: YAMASAKI Kazuo 旭川医科大学, 医学部, 講師 (60241428)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 生体エネルギー転換 / カルシウムポンプ / 筋小胞体 / 静電的相互作用 / イオン輸送 / 酵素反応速度論 / 部位特異的変異 / Ptype ATPase / 機能構造連関

Research Abstract

I explored the roles of electrostatic interactions in the movement of cytoplasmic domain of sarcoplasmic reticulum calcium pump by using a kinetic analysis of site-directed mutants. In this study, I found a linear relationship between logarithm of rate constant and square of the activity coefficient. This plot revealed that electrostatic interactions between domains act like a guide line of domain motion. This plot would provide a useful tool for quantification of electrostatic effects in the enzyme reactions.

Research Products

• [Journal Article] Ca^<2+> Release to Lumen fromADP-sensitive Phosphoenzyme E1PCa_2without Bound K+ of SarcoplasmicReticulum Ca_<2+>-ATPase (2010)
  • Author(s): Kazuo Yamasaki, Takashi Daiho, Stefania Danko, and Hiroshi Suzuki
  • Journal Title: Journal ofBiological Chemistry Volume: 285巻 Issue: 49 Pages: 38674-38683
  • DOI: 10.1074/jbc.m110.183343
  • Peer Reviewed
• [Journal Article] Stable Structural Analog of Ca^<2+>-ATPase ADP-insensitive Phosphoenzyme with Occluded Ca^<2+> Formed by Elongation of A-domain/M1'-linker and Beryllium Fluoride Binding (2010)
  • Author(s): Takashi Daiho, Stefania Danko, KazuoYamasaki, and Hiroshi Suzuki
  • Journal Title: Journal of Biological Chemistry Volume: 285巻 Issue: 32 Pages: 24538-24547
  • DOI: 10.1074/jbc.m110.144535
  • Peer Reviewed
• [Journal Article] Stable Structural Analog of Ca^<2+>-ATPase ADP-insensitive Phosphoenzyme with Occluded Ca^<2+> Formed by Elongation of A-domain/M1'-linker and Beryllium Fluoride Binding (2010)
  • Author(s): Takashi Daiho, Stefania Danko, Kazuo Yamasaki, Hiroshi Suzuki
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 24538-24547
  • Peer Reviewed
• [Journal Article] Ca^2+ Release to Lumen from ADP-sensitive Phosphoenzyme E1PCa_2 wit hout Bound K^+ of Sarcoplasmic Reticulum Ca^<2+>-ATPase (2010)
  • Author(s): Kazuo Yamasaki, Takashi Daiho, Stefania Danko, Hiroshi Suzuki
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 38674-38683
  • NAID: 120004606774
  • Peer Reviewed
• [Journal Article] Roles of Interaction between Actuator and Nucleotide Binding Domains of Sarco(endo)plasmic Reticulum Ca^<2+>-ATPase as Revealed by Single and Swap Mutational Analyses of Serine 186 and Glutamate 439 (2009)
  • Author(s): Xiaoyu Liu, Takashi Daiho, Kazuo Yamasaki, Guoli Wang, Stefania Danko, Hiroshi Suzuki
  • Journal Title: Journal of Biological Chemistry 284 Pages: 25190-25198
  • Peer Reviewed
• [Journal Article] Formation of the Stable Structural Analog of ADP-sensitive Phosphoenzyme of Ca^<2+>-ATPase with Occluded Ca^<2+> by Beryllium Fluoride : STRUCTURAL CHANGES DURING PHOSPHORYLATION AND ISOMERIZATION (2009)
  • Author(s): Stefania Danko, Takashi Daiho, Kazuo Yamasaki, Xiaoyu Liu, Hiroshi Suzuki
  • Journal Title: Journal of Biological Chemistry 284 Pages: 22722-22735
  • Peer Reviewed
• [Journal Article] 筋小胞体Ca^<2+>-ATPaseのE1P-E2P転換とCa^<2+>輸送の共役におけるK^+の役割 (2009)
  • Author(s): 山崎和生, 他
  • Journal Title: 生化学 臨時増刊号 81 Pages: 249-249
• [Journal Article] 高濃度ATPによる筋小胞体Ca^<2+>-ATPaseリン酸化中間体分解の促進効果について ; Actuator/M1-linkerを伸長させた変異体を用いた解析 (2009)
  • Author(s): 大保貴嗣, 他
  • Journal Title: 生化学 臨時増刊号 81 Pages: 249-249
• [Journal Article] 筋小胞体Ca^<2+>-ATPaseのP-N hingeに位置する静電的相互作用の役割についての研究 (2009)
  • Author(s): 山崎和生
  • Journal Title: 旭川医科大学研究フォーラム 10 Pages: 79-80
• [Presentation] 筋小胞体Ca^<2+>-ATPaseリン酸化中間体転換ステップにおける静電相互作用の効果とその定量化の試み (2011)
  • Author(s): 山崎和生
  • Organizer: 生体エネルギー研究会第37回討論会
  • Place of Presentation: 京都
  • Year and Date: 2011-12-20
• [Presentation] 筋小胞体Ca2+-ATPaseリン酸化中間体転換ステップにおける静電相互作用の効果とその定量化の試み (2011)
  • Author(s): 山崎和生
  • Organizer: 生体エネルギー研究会第37回討論会
  • Place of Presentation: 京都(口頭)
  • Year and Date: 2011-12-20
• [Presentation] 筋小胞体Ca2+ポンプにおけるエネルギー共役;細胞質領域アクチュエータードメインと膜貫通へリックスとの連結部分の役割 (2011)
  • Author(s): 大保貴嗣
  • Organizer: 生体エネルギー研究会第37回討論会
  • Place of Presentation: 京都(口頭)
  • Year and Date: 2011-12-20
• [Presentation] Critical importance of helix-loop conversions, length, and Actuator-domain junction structure of M2 in Ca2+-ATPase for Ca2+transport (2011)
  • Author(s): Takashi Daiho
  • Organizer: 13^<th> International P-type ATPase Meeting "Na,K-ATPase and and related P-ATPases^*Structure, Biology and Medicine"
  • Place of Presentation: Pacific Grove, CA, U.S.A.(Oral)
  • Year and Date: 2011-09-29
• [Presentation] Mechanism of Ca2+-ATPase revealed by mutations, kinetics, and development of stable analogs of phosphorylated intermediates (2011)
  • Author(s): Hiroshi Suzuki
  • Organizer: 13^<th> International P-type ATPase Meeting "Na,K-ATPase and and related P-ATPases^*Structure, Biology and Medicine"
  • Place of Presentation: Pacific Grove, CA, U.S.A.(Oral)(招待講演)
  • Year and Date: 2011-09-28
• [Presentation] Roles of electrostaticin teractions between cytoplasmicdomains on the phosphoenzyme transition of Ca^<2+>-ATPase and acritical importance of the chargedresidues at the hinge of the P and Ndomains (2011)
  • Author(s): Kazuo Yamasaki
  • Organizer: 13th International P-typeATPase Meeting Na, K-ATPase and andrelated P-ATPases : Structure, Biologyand Medicine
  • Place of Presentation: Pacific Grove, CA, U.S.A.
• [Presentation] Roles of electrostatic interactions between cytoplasmic domains on the phosphoenzyme transition of Ca2+-ATPase and a critical importance of the charged residues at the hinge of the P and N domains (2011)
  • Author(s): Kazuo Yamasaki
  • Organizer: 13^<th> International P-type ATPase Meeting "Na,K-ATPase and and related P-ATPases^*Structure, Biology and Medicine"
  • Place of Presentation: Pacific Grove, CA, U.S.A.(Poster)
• [Presentation] 筋小胞体Ca^<2+>-ATPaseのリン酸化中間体転換過程における静電的相互作用の役割 (2010)
  • Author(s): 山崎和生
  • Organizer: 第83回日本生化学会第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] 筋小胞体Ca^<2+>-ATPaseのリン酸化中間体の異性化と加水分解に影響を及ぼす構造因子 (2010)
  • Author(s): 大保貴嗣
  • Organizer: 第83回日本生化学会第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] 筋小胞体Ca^<2+>-ATPaseの反応サイクルにおける静電的相互作用の役割とイオン強度依存性との関連 (2010)
  • Author(s): 山崎和生
  • Organizer: 生体エネルギー研究会第36回討論会
  • Place of Presentation: 吹田
  • Year and Date: 2010-11-19
• [Presentation] 筋小胞体Ca^<2+>-ATPaseによるCa^<2+>輸送;細胞質領域アクチュエータードメインお膜貫通ヘリックスを結ぶループの役割 (2010)
  • Author(s): 大保貴嗣
  • Organizer: 生体エネルギー研究会第36回討論会
  • Place of Presentation: 吹田
  • Year and Date: 2010-11-19
• [Presentation] 筋小胞体Ca^<2+>-ATPaseのE1P-E2P転換とCa^<2+>輸送の共役におけるK^+の役割 (2009)
  • Author(s): 山崎和生
  • Organizer: 日本生体エネルギー研究会第35回討論会
  • Place of Presentation: 旭川
  • Year and Date: 2009-12-20
• [Presentation] 筋小胞体Ca^<2+>-ATPaseのリン酸化中間体転換とCa^<2+>輸送;細胞質領域アクチュエータードメインと膜貫通ヘリックスを結ぶループの役割 (2009)
  • Author(s): 大保貴嗣
  • Organizer: 日本生体エネルギー研究会第35回討論会
  • Place of Presentation: 旭川
  • Year and Date: 2009-12-20
• [Presentation] 筋小胞体Ca^<2+>-ATPaseのE1P-E2P転換とCa_2+輸送の共役におけるK^+の役割 (2009)
  • Author(s): 山崎和生
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 筋小胞体Ca^<2+>-ATPaseのE1P-E2P転換とCa^<2+>輸送の共役におけるK^+の役割 (2009)
  • Author(s): 山崎和生
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] 高濃度ATPによる筋小胞体Ca^<2+>-ATPaseリン酸化中間体分解の促進効果にういて;Actuator/M1-linkerを伸長させた変異体を用いた解析 (2009)
  • Author(s): 大保貴嗣
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22