Analysis of subcellular region-specific signaling by Ras and Rho family GTPases

• Project/Area Number: 21570138
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Osaka Prefecture University (2011); Kobe University (2009-2010)
• Principal Investigator: SATOH Takaya 大阪府立大学, 大学院・理学系研究科, 教授 (20251655)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: ゲノム / 細胞・組織 / シグナル伝達 / 蛋白質 / Rac1 / インスリン / 糖取り込み / 骨格筋 / Racl / Rho / Rapl / 血管形成 / 中心体

Research Abstract

Ras and Rho family GTPases play an important role as a molecular switch in intracellular signal transduction. Recently, its subcellular region-specific regulatory mechanisms are featured. In this study, we analyzed the subcellular region-specific regulatory mechanism underlying newly identified signaling pathways involving the GTPase Rac1, RhoA, or Rap1.

Research Products

• [Journal Article] RA-GEF-1(Rapgef2) is essential for proper development of the midline commissures (2011)
  • Author(s): Shymaa E. Bilasy, Takaya Satoh, Toshio Terashima, and Tohru Kataoka
  • Journal Title: Neurosci. Res Volume: 71(3) Pages: 200-209
  • Peer Reviewed
• [Journal Article] RA-GEF-1 (Rapgef2) is essential for proper development of the midline commissures (2011)
  • Author(s): Shymaa E.Bilasy
  • Journal Title: Neurosci.Res. Volume: 71 Pages: 200-209
  • Peer Reviewed
• [Journal Article] Crucial role of the small GTPase Rac1 in insulin-stimulated translocation of glucose transporter 4 to the mouse skeletal muscle sarcolemma (2010)
  • Author(s): Shuji Ueda, Sohei Kitazawa, Kota Ishida, Yuki Nishikawa, Megumi Matsui, Hikaru Matsumoto, Takuji Aoki, Shinsuke Nozaki, Tomoya Takeda, Yoshikazu Tamori, Atsu Aiba, C. Ronald Kahn, Tohru Kataoka, and Takaya Satoh
  • Journal Title: FASEB J Volume: 24(7) Pages: 2254-2261
  • Peer Reviewed
• [Journal Article] Crucial role of the small GTPase Racl in insulin-stimulated translocation of glucose transporter 4 to the mouse skeletal muscle sarcolemma (2010)
  • Author(s): Shuji Ueda
  • Journal Title: FASEB J. Volume: 24 Pages: 2254-2261
  • Peer Reviewed
• [Journal Article] Crucial role of the small GTPase Rac1 in insulin-stimulated translocation of glucose transporter 4 to the mouse skeletal muscle sarcolemma (2010)
  • Author(s): Shuji Ueda
  • Journal Title: FASEB J. (in press(掲載確定))
  • Peer Reviewed
• [Journal Article] Mixed-lineage-leukemia(MLL) fusion protein collaborates with Ras to induce acute leukemia through aberrant Hox expression and Raf activation (2009)
  • Author(s): Ryoichi Ono, Hidetoshi Kumagai, Hideaki Nakajima, Ai Hishiya, Tomohiko Taki, Keisuke Horikawa, Kiyoshi Takatsu, Takaya Satoh, Yasuhide Hayashi, Toshio Kitamura, and Tetsuya Nosaka
  • Journal Title: Leukemia Volume: 23(12) Pages: 2197-2209
  • Peer Reviewed
• [Journal Article] Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1 (2009)
  • Author(s): Hoshimi Kanemura, Takaya Satoh, Shymaa E. Bilasy, Shuji Ueda, Masanori Hirashima, and Tohru Kataoka
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 387(4) Pages: 754-759
  • Peer Reviewed
• [Journal Article] Regulation of mitotic spindle formation by the RhoA guanine nucleotide exchange factor ARHGEF10 (2009)
  • Author(s): Takuji Aoki, Shuji Ueda, Tohru Kataoka, and Takaya Satoh
  • Journal Title: BMC Cell Biol Volume: 10 Pages: 56-56
  • Peer Reviewed
• [Journal Article] Dorsal telencephalon-specific RA-GEF-1 knockout mice develop heterotopic cortical mass and commissural fiber defect (2009)
  • Author(s): Shymaa E. Bilasy, Takaya Satoh, Shuji Ueda, Ping Wei, Hoshimi Kanemura, Atsu Aiba, Toshio Terashima, and Tohru Kataoka
  • Journal Title: Eur. J. Neurosci Volume: 29(10) Pages: 1994-2008
  • Peer Reviewed
• [Journal Article] Dorsal telencephalon-specific RA-GEF-1 knockout mice develop heterotopic cortical mass and commissural fiber defect (2009)
  • Author(s): Shymaa E.Bilasy
  • Journal Title: Eur.J.Neurosci. 29 Pages: 1994-2008
  • Peer Reviewed
• [Journal Article] Regulation of mitotic spindle formation by the RhoA guanine nucleotide exchange factor ARHGEF10 (2009)
  • Author(s): Takuji Aoki
  • Journal Title: BMC Cell Biol. 10 Pages: 56-56
  • Peer Reviewed
• [Journal Article] Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1 (2009)
  • Author(s): Hoshimi Kanemura
  • Journal Title: Biochem.Biophys.Res.Commun. 387 Pages: 754-759
  • Peer Reviewed
• [Journal Article] Mixed-lineage-leukemia(MLL)fusion protein collaborates with Ras to induce acute leukemia through aberrant Hox expression and Raf activation (2009)
  • Author(s): Ryoichi Ono
  • Journal Title: Leukemia 23 Pages: 2197-2209
  • Peer Reviewed
• [Presentation] 骨格筋へのインスリン応答性糖取り込みにおけるRac1の機能解析 (2011)
  • Author(s): 野崎真輔, 武田朋也, 北浦拓也, 片岡徹, 佐藤孝哉
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] The Rap1 guanine nucleotide exchange factor RA-GEF-1 is essential for the proper development of the mouse cerebral cortex (2011)
  • Author(s): Kaori Nishihara, Shymaa E. Bilasy, Hironori Edamatsu, Toshio Terashima, Yuichi Ijiri, Takaya Satoh, and Tohru Kataoka
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Crucial role of the small GTPase Rac1 in insulin-stimulated translocation of glucose transporter 4 to the mouse skeletal muscle sarcolemma (2011)
  • Author(s): Shuji Ueda, Sohei Kitazawa, Kota Ishida, Yuki Nishikawa, Megumi Matsui, Hikaru Matsumoto, Takuji Aoki, Shinsuke Nozaki, Tomoya Takeda, Yoshikazu Tamori, Atsu Aiba, C. Ronald Kahn, Tohru Kataoka, and Takaya Satoh
  • Organizer: 2011 Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: Denver, CO, USA
  • Year and Date: 2011-12-04
• [Presentation] Crucial role of the small GTPase Rac1 in insulin-stimulated translocation of glucose transporter 4 to the mouse skeletal muscle sarcolemma (2011)
  • Author(s): Shuji Ueda
  • Organizer: 2011 Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: アメリカ合衆国・コロラド州・デンバー
  • Year and Date: 2011-12-04
• [Presentation] 大脳皮質の交連線維形成におけるグアニンヌクレオチド交換因子RA-GEF-1の機能 (2011)
  • Author(s): 寺島俊雄, シャイマー・ビラジィ, 薛富義, 佐藤孝哉, 片岡徹
  • Organizer: 第88回日本生理学会大会・第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] The Rap1 guanine nucleotide exchange factor RA-GEF-1 is essential for the proper development of the mouse dorsal telencephalon (2011)
  • Author(s): Shymaa E. Bilasy, Takaya Satoh, Toshio Terashima, and Tohru Kataoka
  • Organizer: 31st Annual Meeting of the Australian Neuroscience Society
  • Place of Presentation: Auckland, New Zealand
  • Year and Date: 2011-02-03
• [Presentation] The Rap1 guanine nucleotide exchange factor RA-GEF-1 is essential for the proper development of the mouse dorsal telencephalon. (2011)
  • Author(s): Shymaa E.Bilasy
  • Organizer: 31st Annual Meeting of the Australian Neuroscience Society
  • Place of Presentation: Auckland (New Zealand)
  • Year and Date: 2011-02-03
• [Presentation] 骨格筋へのインスリン応答性糖取り込みにおけるRac1の機能解析 (2010)
  • Author(s): 野崎真輔, 武田朋也, 北浦拓也, 片岡徹, 佐藤孝哉
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] The Rap1 guanine nucleotide exchange factor RA-GEF-1 is essential for the proper development of the mouse cerebral cortex (2010)
  • Author(s): Shymaa E. Bilasy, Takaya Satoh, Toshio Terashima, and Tohru Kataoka
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] The Rap1 guanine nucleotide exchange factor RA-GEF-1 is essential for the proper development of the mouse cerebral cortex. (2010)
  • Author(s): Shymaa E.Bilasy
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] 骨格筋へのインスリン応答性糖取り込みにおけるRac1の機能解析. (2010)
  • Author(s): 野崎真輔
  • Organizer: 第83回日本生化学会大会・第33回日本分子生物学会年会合同大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] The Rap1 guanine nucleotide exchange factor RA-GEF-1 is essential for the proper development of the mouse cerebral cortex (2010)
  • Author(s): Shymaa E. Bilasy, Takaya Satoh, Toshio Terashima, and Tohru Kataoka
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-04
• [Presentation] The Rap1 guanine nucleotide exchange factor RA-GEF-1 is essential for the proper development of the mouse cerebral cortex. (2010)
  • Author(s): Shymaa E.Bilasy
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
  • Year and Date: 2010-09-04
• [Presentation] Regulation of mitotic spindle formation by the RhoA guanine nucleotide exchange factor ARHGEF10 (2009)
  • Author(s): Takuji Aoki, Shuji Ueda, Tohru Kataoka, and Takaya Satoh
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Regulation of mitotic spindle formation by the RhoA guanine nucleotide exchange factor ARHGEF10 (2009)
  • Author(s): Takuji Aoki
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] マウス胎生期の血管形成におけるRA-GEF-1の機能解析 (2009)
  • Author(s): 金村星余, 佐藤孝哉, 上田修司, Shymaa E. Bilasy, 平島正則, 片岡徹
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-22
• [Presentation] マウス胎生期の血管形成におけるRA-GEF-1の機能解析 (2009)
  • Author(s): 金村星余
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-22
• [Presentation] 背側終脳特異的RA-GEF-1ノックアウトマウスは大脳皮質ヘテロトピアと交連線維系の欠損を示す (2009)
  • Author(s): 寺島俊雄, Shymaa E. Bilasy, 佐藤孝哉, 上田修司, Ping Wei, 金村星余, 饗場篤, 片岡徹
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] 背側終脳特異的RA-GEF-1ノックアウトマウスは大脳皮質ヘテロトピアと交連線維系の欠損を示す (2009)
  • Author(s): 寺島俊雄
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-16
• [Book] Encyclopedia of Signaling Molecules (2012)
  • Author(s): Takaya Satoh
  • Publisher: Springer(印刷中)
• [Book] 低分子量Gタンパク質 (2012)
  • Author(s): 佐藤孝哉
  • Publisher: 羊土社(印刷中)
• [Book] デブリン生化学(第7版) (2012)
  • Author(s): 佐藤孝哉
• [Book] キーワードで理解するシグナル伝達イラストマップ(改訂版) (2012)
  • Author(s): 佐藤孝哉
  • Publisher: 羊土社(印刷中)
• [Book] シグナル伝達.生命システムの情報ネットワーク.(第2版) (2011)
  • Author(s): 佐藤孝哉
• [Book] シグナル伝達-生命システムの情報ネットワーク-(第2版)(翻訳) (2011)
  • Author(s): 上代淑人, 佐藤孝哉監訳
  • Total Pages: 678
  • Publisher: メディカル・サイエンス・インターナショナル
• [Book] Encyclopedia of Signaling Molecules (2011)
  • Author(s): Takaya Satoh
  • Publisher: Springer(未定)
• [Book] シグナル伝達-生命システムの情報ネットワーク-(第2版)(翻訳) (2011)
  • Author(s): 上代淑人, 佐藤孝哉 監訳
  • Publisher: メディカル・サイエンス・インターナショナル(未定)
• [Remarks]
  • URL: http://www.b.s.osakafu-u.ac.jp/
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/molbiol/index.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/molbiol/index.html