Analysis of DG kinase functions related to PKC and its application for future drug.
Project/Area Number |
21570139
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Kobe University |
Principal Investigator |
SHIRAI Yasuhito 神戸大学, 大学院・農学研究科, 教授 (60263399)
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Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 細胞情報伝達機構 / 脂質 / ジアシルグリセロールキナーゼ / プロテインキナーゼC / 脂質シグナル / 神経 / 糖尿病 / スパイン / LTP / 腎症 / 脂質キナーゼ / ジアシルグリセロール / フォスファチジン酸 / 記憶 / KOマウス |
Research Abstract |
To elucidate function of DGK. in neurons, we developed DGK. KO mice using the Sleeping Beauty transposon system, and found that DGK. regulates spine formation by regulation of lipids, contributing to the maintenance of neural networks in synaptic transmission of cognitive processes including memory and emotion. Using DGK. KO mice, we investigated physiological role of DGK. in Vitamin E-induced improvement of diabetic renal dysfunction, and found that renal dysfunction of DGK. KO mice was sever and occurred earlier than WT. Finally, we discovered c-Src phosphorylated Tyr22 and Tyr334, while c-Abl phosphorylated 218Tyr.
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Report
(4 results)
Research Products
(57 results)
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[Journal Article] c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase・by phosphorylation at Tyr2182012
Author(s)
Matsubara, M., Ikeda, M., Kiso, Y., Sakuma, M., Sakane, F., Merida, I, Saito, N. and Shirai, Y.
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Journal Title
J. Biol. Chem.
Volume: 287
Pages: 5507-5517
Related Report
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[Journal Article] Direct biding of RalA to PKC・and its crucial role in morphological change during keratinocytes differentiation2011
Author(s)
Shirai, Y., Morioka, S., Sakuma, M., Yoshino, K., Otsuji, C., Sakai, N., Kashiwagi, K., Chida, K., Shirawaka, R., Horiuchi, H., Nishigori, C., Ueyama, T. and Saito, N
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Journal Title
Mol. Biol. Cell
Volume: 22
Pages: 1340-1352
Related Report
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[Journal Article] c-Abl tyrosine kinase regulates serum-induced nuclear export of diacylglycerol kinase α by phosphorylation at Tyr2182011
Author(s)
Matsubara, M., Ikeda, M., Kiso, Y., Sakuma, M., Sakane, F., Merida, I, Saito, N., Shirai, Y.
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Journal Title
J.Biol.Chem
Volume: 287
Issue: 8
Pages: 5507-5517
DOI
Related Report
Peer Reviewed
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[Journal Article] Streptococcus mutans diacylglycerol kinase homologue : a potential target for anti-caries chemotherapy2011
Author(s)
Shibata, Y., Kawada-matsuo, M., Shirai, Y., Saito, N., Li, D., Yamashita, Y.
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Journal Title
J.Medical Microbiol.
Volume: 60
Pages: 625-630
NAID
Related Report
Peer Reviewed
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[Journal Article] Direct biding of RalA to PKCη and its crucial role in morphological change during keratinocytes differentiation2011
Author(s)
Shirai, Y., Morioka, S., Sakuma, M., Yoshino, K., Otsuji, C., Sakai, N., Kashiwagi, K., Chida, K., Shirawaka, R., Horiuchi, H., Nishigori, C., Ueyama, T., Saito, N.
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Journal Title
Mol.Biol.Cell
Volume: 22
Pages: 1340-1352
Related Report
Peer Reviewed
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[Journal Article] Diacylglycerol kinase・・knockout mice exhibit lithium-sensitive behavioral abnormalities2010
Author(s)
Kakefuda K., Oyagi A., Ishisaka, M., Tsumura, K., Shimazawa M., Yokota, K., Shirai Y., Horie K., Saito N., Takeda J. and Hara H.
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Journal Title
Related Report
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[Journal Article] Essential role of neuron-enriched diacylglycerol kinase(DGK), DGK・in neurite spine formation, contributing to cognitive function2010
Author(s)
Shirai Y., Kouzuki T., Kakefuda K., Moriguchi S., Oyagi A., Horie K., Morita S., Shimazawa M., Fukunaga K., Takeda J., Saito N. and Hara H
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Journal Title
Related Report
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[Journal Article] DGKβ in neurite spine formation, contributing to cognitive function.2010
Author(s)
Shirai Y., Kouzuki T., Kakefuda K., Moriguchi S., Oyagi A., Horie K., Morita S., Shimazawa M., Fukunaga K., Takeda J., Saito N., Hara H.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Diacylglycerol kinase β knockout mice exhibit lithium-sensitive behavioral abnormalities2010
Author(s)
Kakefuda K., Oyagi A., Ishisaka, M., Tsumura, K., Shimazawa M., Yokota, K. Shirai Y., Horie K., Saito N.,Takeda J., Hara H.
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Journal Title
Related Report
Peer Reviewed
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[Presentation] Impairment of cognitive function and lithium-sensitive behavior of knockout mice of neuron-enriched subtype of DG kinase2011
Author(s)
Y Shirai, T Kouzuki, K Kakefuda, A Ohyagi, K Horie, S Moriguchi, S Morita, M Shimazawa, K Fukunaga, J Takeda, N Saito, H Hara
Organizer
First annual meeting of International Society of Radiation Neurobiology
Place of Presentation
Maebashi, Japan
Year and Date
2011-01-29
Related Report
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[Presentation] Membrane lipids control by neuron-enriched diacylglycerol kinase・is important for spine formation and cognitive function2010
Author(s)
Y. Shirai, T. Kouzuki, K. Kakefuda, S. Moriguchi, A. Oyagi, K. Horie, S. Morita, M. Shimazawa, K. Fukunaga, J. Takeda, N. Saito and H. Hara
Organizer
Bioactive lipids : biochemistry and diseases
Year and Date
2010-06-09
Related Report
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[Presentation] Membrane lipids control by neuron-enriched diacylglycerol kinaseβ is important for spine formation and cognitive function.2010
Author(s)
Y.Shirai, T.kouzuki, K.Kakefuda, S.Moriguchi, A.Oyagi, K.Horie S.Morita, M.Shimazawa, K.Fukunaga, J.Takeda, N.Saito, H.Hara
Organizer
Keystone symposia "Bioactive lipids : biochemistry and diseases
Place of Presentation
ウェスティン都京都(京都府)(招待講演)
Year and Date
2010-06-09
Related Report
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