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Role of Sialic Acid for Angiogenesis

Research Project

Project/Area Number 21570154
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KAWAGUCHI Shinobu  独立行政法人理化学研究所, 疾患糖鎖研究チーム, 副チームリーダー (80301753)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsシアル酸転移酵素 / 血管内皮細胞 / ST6Gal I / PECAM / シアル酸 / α2,6-シアル酸 / アポトーシス / SHP2 / 接着分子 / 血管新生 / ITIM
Research Abstract

In this study, we show thatα2, 6-sialic acid is necessary for the cell-surface residency of platelet endothelial cell adhesion molecule(PECAM), a member of the immunoglobulin superfamily that plays multiple roles in cell adhesion, mechanical stress sensing, antiapoptosis and angiogenesis. As a possible underlying mechanism, we found that the homophilic interactions of PECAM in endothelial cells were dependent onα2, 6-sialic acid. We also found that the absence ofα2, 6-sialic acid downregulated the tyrosine-phosphorylation of PECAM and recruitment of SHP2, and rendered the cells more prone to mitochondria-dependent apoptosis, as evaluated using PECAM-deficient endothelial cells.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (44 results)

All 2011 2010 2009 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (13 results) Book (5 results) Remarks (5 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 2 results)

  • [Journal Article] How does N. and O-glycosylation affect the formation and accumulation of Amyloidβ-peptide?2011

    • Author(s)
      S. Kitazume
    • Journal Title

      Trends in Glycoscience and Glycotechnology

      Volume: 23 Pages: 161-167

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Brain-specific expression of N-acetylglucosaminyltransferase IX(GnT-IX) is regulated by epigenetic histone modifications2011

    • Author(s)
      Y. Kizuka, S. Kitazume, M. Yoshida, and N. Taniguchi
    • Journal Title

      Biol. Chem.

      Volume: 286 Pages: 31875-31884

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A unique N-glycan on human transferrin in CSF : a possible biomarker for iNPH2011

    • Author(s)
      S. Futakawa, K. Nara, M. Miyajima, A. Kuno, H. Ito, H. Kaji, K. Shirotani, T. Honda, Y. Tohyama, K. Hoshi, Y. Hanzawa, S. Kitazume, R. Imamaki, K. Furukawa, K. Tasaki, H. Arai, T. Yuasa, M. Abe, H. Arai, H. Narimatsu, and Y. Hashimoto
    • Journal Title

      Neurobiol. Aging

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Brain-specific expression of N-acetylglucosaminyltransferase IX (GnT-IX) is regulated by epigenetic histone modifications2011

    • Author(s)
      Y.Kizuka, S.Kitazume, M.Yoshida, N.Taniguchi
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Issue: 36 Pages: 31875-31884

    • DOI

      10.1074/jbc.m111.251173

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brain endothelial cells produce amyloidβfrom amyloid precursor protein 770 and preferentially secrete the O-glycosylated form2010

    • Author(s)
      S. Kitazume, Y. Tachida, M. Kato, Y. Yamaguchi, T. Honda, Y. Hashimoto, Y. Wada, T. Saito, N. Iwata, T. Saido and N, Taniguchi
    • Journal Title

      J. Biol. Chem.

      Volume: 285 Pages: 40097-40103

    • URL

      http://www.functionalglycomics.org/fg/update/2010/101111/full/fg.2010.36.shtml

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous determination of nucleotide sugars involved in glycosylation with ion-pair reversed. phase HPLC2010

    • Author(s)
      K. Nakajima, S. Kitazume, T. Angata, R. Fujinawa, E. Miyoshi, and N. Taniguchi
    • Journal Title

      Glycobiology

      Volume: 20 Pages: 865-871

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] α2, 6-Sialic Acid on Platelet Endotheli al Cell Adhesion Molecule(PECAM) Regulates its homophilic interactio ns and downstream antiapoptotic si gnaling2010

    • Author(s)
      S. Kitazume, R. Imamaki, K. Ogaw a, Y. Komi, S. Futakawa, S. Kojima, Y. Hashimoto, J. D. Marth, J. C. Paulson, and N. Taniguchi
    • Journal Title

      J. Biol. Chem.

      Volume: 285 Pages: 6515-6521

    • URL

      http://www.functionalglycomics.org/fg/update/2010/100211/full/fg.2010.8.shtml

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Brain endothelial cells produce amyloid β from amyloid precursor protein 770 and preferentially secrete the O-glycosylated form2010

    • Author(s)
      S.Kitazume, Y.Tachida, M.Kato, Y.Yamaguchi, T.Honda, Y.Hashimoto, Y.Wada, T.Saito, N.Iwata, T.Saido, N, Taniguchi
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 40097-40103

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous determination of nucleotide sugars involved in glycosylation with ion-pair reversed-phase HPLC2010

    • Author(s)
      K.Nakajima, S.Kitazume, T.Angata, R.Fujinawa, E.Miyoshi, N.Taniguchi
    • Journal Title

      Glycobiology

      Volume: 20 Pages: 865-871

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] α2, 6-Sialic Acid on Platelet Endothelial Cell Adhesion Molecule (PECAM) Regulates Its Homophilic Interactions and Downstream Antiapoptotic Signaling2010

    • Author(s)
      S.Kitazume, R.Imamaki, K.Ogawa, Y.Komi, S.Futakawa, S.Kojima, Y.Hashimoto, J.D.Marth, J.C.Paulson, N.Taniguchi
    • Journal Title

      J.Biol.Chem

      Volume: 285 Pages: 6515-6521

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulates Its Homophilic Interactions and Downstream Antiapoptotic Signaling2010

    • Author(s)
      S.Kitazume, R.Imamaki, K.Ogawa, Y.Komi, S.Futakawa, S.Kojima, Y.Hashimoto, J.D.Marth, J.C.Paulson, N.Taniguchi
    • Journal Title

      J.Biol.Chem. 285(In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular insights intoβ-galactosideα2, 6-sialyltransferase secretion in vivo2009

    • Author(s)
      S. Kitazume#, R. Oka, K. Ogawa, S. Futakawa, Y. Hagiwara, H. Takigawa, M. Kato, A. Kasahara, E. Miyoshi, N. Taniguchi, and Y. Hashimoto
    • Journal Title

      Glycobiology

      Volume: 19 Pages: 479-487

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of sandwich enzyme-linked immunosorbent assay systems for plasmaβ-galactosideα2, 6-sialyltransferase, a possible hepatic disease biomarker2009

    • Author(s)
      S. Futakawa, S. Kitazume, R. Oka, K. Ogawa, Y. A. Kinoshita, K. Miyashita and Y. Hashimoto
    • Journal Title

      Analytica Chimica Acta

      Volume: 531 Pages: 116-120

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular insights into β-galactoside α2, 6-sialyltransferase secretion in vivo2009

    • Author(s)
      S.Kitazume, R.Oka, K.Ogawa, S.Futakawa, Y.Hagiwara, H.Takigawa, M.Kato, A.Kasahara, E.Miyoshi, N.Taniguchi, Y.Hashimoto
    • Journal Title

      Glycobiology 19

      Pages: 479-487

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of sandwich enzyme-linked immunosorbent assay systems for plasma β-galactoside α2, 6-sialyltransferase, a possible hepatic disease biomarker2009

    • Author(s)
      S.Futakawa, S.Kitazume, R.Oka, K.Ogawa, Y.A.Kinoshita, K.Miyashita, Y.Hashimoto
    • Journal Title

      Analytica Chimica Acta 531

      Pages: 116-120

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Integrated approach toward the discovery of glyco-biomarkers of in flammation-related diseases

    • Author(s)
      Takashi Angata, Reiko Fujinawa, Ayako Kurimoto, Kazuki Nakajima, Masaki Kato, Shinji Takamatsu, Hiroaki Korekane, Cong-Xiao Gao, Kazuaki Ohtsubo, Shinobu Kitazume, Naoyuki Taniguchi
    • Journal Title

      Annals of New York Academy of Sciences

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid : A Possible Extension toward Alzheimer's Disease Biomarker Development

    • Author(s)
      K.Shirotani, S.Futakawa, K.Nara, K.Hoshi, T.Saito, Y.Tohyama, S.Kitazume, T.Yuasa, M.Miyajima, H, Arai, A.Kuno, H.Narimatsu, Y.Hashimoto
    • Journal Title

      International Journal of Alzheimer's Disease

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Brain endothelial cells produce amyloidβfrom amyloid precursor protein 770 and preferentially secrete the O-glycosylated form2011

    • Author(s)
      S. Kitazume
    • Organizer
      第32回日本生化学会シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] Brain endothelial cells produce amyloid β from amyloid precursor protein 770 and preferentially secrete the O-glycosylated form2011

    • Author(s)
      Shinobu Kitazume
    • Organizer
      第32回日本生化学会シンポジウム
    • Place of Presentation
      京都(招待講演)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] α2, 6-Sialic Acid on Platelet Endothelial Cell Adhesion Molecule(PECAM) Regulates Its Homophilic Interactions and Downstream Antiapoptotic Signaling2010

    • Author(s)
      S. Kitazume, R. Imamaki, K. Ogawa, Y. Komi, S. Futakawa, S. Kojima, Y. Hashimoto, J. D. Marth, J. C. Paulson, and N. Taniguchi
    • Organizer
      ICS2010
    • Place of Presentation
      Chiba
    • Year and Date
      2010-08-08
    • Related Report
      2011 Final Research Report
  • [Presentation] α2, 6-Sialic Acid on Platelet Endothelial Cell Adhesion Molecule (PECAM) Regulates Its Homophilic Interactions and Downstream Antiapoptotic Signaling2010

    • Author(s)
      S.Kitazume, R.Imamaki, K.Ogawa, Y.Komi, S.Futakawa, S.Kojima, Y.Hashimoto, J.D.Marth, J.C.Paulson, N.Taniguchi
    • Organizer
      ICS2010
    • Place of Presentation
      千葉県・幕張
    • Year and Date
      2010-08-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Brain endothelial cells produce amyloid b from amyloid precursor protein 770 and preferentially secrete the O-glycosylated form2010

    • Author(s)
      S. Kitazume
    • Organizer
      Glyco Tokyo 2010
    • Place of Presentation
      Tokyo
    • Year and Date
      2010-07-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Brain endothelial cells produce amyloid β from amyloid precursor protein 770 and preferentially secrete the O-glycosylated form2010

    • Author(s)
      Shinobu Kitazume
    • Organizer
      GlycoT2010
    • Place of Presentation
      東京都・浅草
    • Year and Date
      2010-07-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] α2, 6-Sialic Acid on Platelet Endothelial Cell Adhesion Molecule(PECAM) Regulates Its Homophilic Interactions and Downstream Antiapoptotic Signaling2010

    • Author(s)
      S. Kitazume
    • Organizer
      The 2nd Japan/China Joint Symposium on Glycobiology
    • Place of Presentation
      Wako
    • Year and Date
      2010-04-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Anα2, 6-Sialic Acid on Platelet Endothelial Cell Adhesion Molecule(PECAM) Regulates Its Homophilic Interactions and Downstream Anti-apoptotic Signaling2010

    • Author(s)
      S. Kitazume
    • Organizer
      Joint Japan-CCRC Symposium : New insights into the dynamic functions of glycans
    • Place of Presentation
      Athens(U. S. A)
    • Year and Date
      2010-03-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Sialic acid-based homophilic PECAM interactions regulate pathological angiogenesis2009

    • Author(s)
      S. Kitazume
    • Organizer
      第32回日本分子生物学会シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Sialic acid-based homophilic PECAM interactions regulate pathological angiogenesis2009

    • Author(s)
      北爪しのぶ
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] Sialic acid-based homophilic PECAM interactions and pathological angiogenesis2009

    • Author(s)
      Shinobu Kitazume, Rie Imamaki, Kazuko Ogawa, Yusuke Komi, Satoshi Futakawa, Soichi Kojima, Yasuhiro Hashimoto, Jamey D. Marth, James C. Paulson, and Naoyuki Taniguchi
    • Organizer
      2009 Annual Meeting of the Society for Glycobiology
    • Place of Presentation
      san Diego
    • Year and Date
      2009-11-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 血管新生におけるα2, 6-シアル酸の役割2009

    • Author(s)
      北爪しのぶ、今牧理恵、小川加寿子、小見悠介、小嶋聡一、橋本康弘、Jamey D. Marth、谷口直之
    • Organizer
      第29回日本糖質学会
    • Place of Presentation
      飛騨
    • Year and Date
      2009-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] 血管新生におけるα2, 6-シアル酸の役割2009

    • Author(s)
      北爪しのぶ、今牧理恵、小川加寿子、小見悠介、小嶋聡一、橋本康弘、Jamey D.Marth、谷口直之
    • Organizer
      第29回日本糖質学会
    • Place of Presentation
      飛騨・世界生活文化センター(岐阜)
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Book] How does Nand O-glysocylation affect the formation and accumulation of amyloideυ-peptide?2011

    • Author(s)
      Shinobu Kitazume
    • Related Report
      2011 Final Research Report
  • [Book] GnT-IX遺伝子の脳特異的な発現とアイソフォーム「脳21」2011

    • Author(s)
      木塚康彦、兼清健志、北爪しのぶ、谷口直之
    • Related Report
      2011 Final Research Report
  • [Book] 「脳21」GnT-IX遺伝子の脳特異的な発現とアイソフォーム2011

    • Author(s)
      木塚康彦、兼清健志、北爪しのぶ、谷口直之
    • Total Pages
      85
    • Publisher
      メディカルオンライン
    • Related Report
      2010 Annual Research Report
  • [Book] アルツハイマー病の原因酵素が糖鎖を変えていた、「糖鎖の生物機能の解明と利用技術」2009

    • Author(s)
      橋本康弘、北爪しのぶ
    • Related Report
      2011 Final Research Report
  • [Book] アルツハイマー病の原因酵素が糖鎖を変えていた、「糖鎖の生物機能の解明と利用技術」2009

    • Author(s)
      橋本康弘、北爪しのぶ
    • Total Pages
      12
    • Publisher
      蛋白質・核酸・酵素
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.functionalglycomics.org/fg/update/2010/100211/full/fg.2010.8.shtml

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.functionalglycomics.org/fg/update/2010/100211/full/fg.2010.8.shtml

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.functionalglycomics.org/fg/archive/2010/11/fa.shtml

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.riken.go.jp/r-world/research/results/2010/100302/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.riken.go.jp/r-world/research/results/2010/101021/index.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 認知症診断の為の可溶型アミロイドβ前駆体タンパク質770β切断産物の検出方法2011

    • Inventor(s)
      北爪しのぶ、谷口直之、立田由里子、西道隆臣、橋本康弘、本多たかし
    • Industrial Property Rights Holder
      理化学研究所
    • Filing Date
      2011-07-29
    • Related Report
      2011 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 認知症診断の為の可溶型アミロイドβ前駆体タンパク質770'β切断産物の検出方法2011

    • Inventor(s)
      北爪しのぶ、谷口直之、立田由里子、西道隆臣、橋本康弘、本多たかし
    • Industrial Property Rights Holder
      理化学研究所
    • Filing Date
      2011-07-29
    • Related Report
      2011 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 認知症診断の為の可溶型アミロイドβ前駆体タンパク質770β切断産物の検出方法2011

    • Inventor(s)
      北爪しのぶ、谷口直之、立田由里子、西道隆臣、橋本康弘、本多たかし
    • Industrial Property Rights Holder
      独立行政法人理化学研究所
    • Industrial Property Number
      2010-171122
    • Filing Date
      2011-07-29
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 認知症診断の為の可溶型アミロイドβ前駆体タンパク質770β切断産物の検出方法2010

    • Inventor(s)
      北爪しのぶ、谷口直之、立田由里子、西道隆臣、橋本康弘、本多たかし
    • Industrial Property Rights Holder
      理化学研究所
    • Acquisition Date
      2010-07-29
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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