| Project/Area Number |
21570154
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KAWAGUCHI Shinobu 独立行政法人理化学研究所, 疾患糖鎖研究チーム, 副チームリーダー (80301753)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | シアル酸転移酵素 / 血管内皮細胞 / ST6Gal I / PECAM / シアル酸 / α2,6-シアル酸 / アポトーシス / SHP2 / 接着分子 / 血管新生 / ITIM |
|---|
| Research Abstract |
In this study, we show thatα2, 6-sialic acid is necessary for the cell-surface residency of platelet endothelial cell adhesion molecule(PECAM), a member of the immunoglobulin superfamily that plays multiple roles in cell adhesion, mechanical stress sensing, antiapoptosis and angiogenesis. As a possible underlying mechanism, we found that the homophilic interactions of PECAM in endothelial cells were dependent onα2, 6-sialic acid. We also found that the absence ofα2, 6-sialic acid downregulated the tyrosine-phosphorylation of PECAM and recruitment of SHP2, and rendered the cells more prone to mitochondria-dependent apoptosis, as evaluated using PECAM-deficient endothelial cells.
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