Regulatory mechanism of glycosylation

• Project/Area Number: 21570157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Keio University (2010-2011); Mitsubishi Kagaku Institute of Life Sciences (2009)
• Principal Investigator: GOTO Satoshi 慶應義塾大学, 医学部, 特任講師 (60280575)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 糖鎖 / mRNA / ショウジョウバエ / 翻訳後修飾 / 小胞体 / 核外輸送 / 転写

Research Abstract

Glycosylation plays an important role in various physiological processes such as development, immunity and neural functions. However, mechanisms regulating glycosylation still remain largely unknown. To elucidate it, we performed a large scale screen using Drosophila RNAi library and identified novel genes. We analyzed the functions of the genes and found novel mechanisms of mRNA of glycosylation related genes.

Research Products

• [Journal Article] Identification of proteasome components required for apical localization of Chaoptin using functional genomics (2012)
  • Author(s): Yano, H., Yamamoto-Hino, M., Awano, W., Aoki-Kinoshita, K. F., Tsuda-Sakurai, K., Okano, H. and Goto, S.
  • Journal Title: J. Neurogenet Volume: vol.26 Issue: 1 Pages: 53-63
  • DOI: 10.3109/01677063.2012.661497
  • Peer Reviewed
• [Journal Article] Multi-stage mass spectrometric information obtained by deconvolution of energy-resolved spectra acquired by triple-quadrupole mass spectrometry (2011)
  • Author(s): O.Kanie, Y.Kanie, S.Daikoku, Y.Shioiri, A.Kurimoto, S.Mutsuga, S.Goto, Y.Ito, K.Suzuki
  • Journal Title: Rapid Communications in Mass Spectrometry Volume: 25 Issue: 11 Pages: 1617-1624
  • DOI: 10.1002/rcm.5031
  • Peer Reviewed
• [Journal Article] Identification of genes required for neural-specific glycosylation using functional genomics (2010)
  • Author(s): Yamamoto-Hino, M., Kanie, Y., Awano, W., Aoki-Kinoshita, K. F., Yano, H., Nishihara, S., Okano, H., Ueda, R., Kanie, O. and Goto, S.
  • Journal Title: PLoS Genetics Volume: vol.6 Issue: 12 Pages: e1001254-e1001254
  • DOI: 10.1371/journal.pgen.1001254
  • Peer Reviewed
• [Journal Article] Identification of genes required for neural-specific glycosylation using functional genomics. (2010)
  • Author(s): Yamamoto-Hino, M., et al.
  • Journal Title: PLoS Genetics Volume: 6
  • Peer Reviewed
• [Journal Article] Balanced ubiquitylation and deubiquitylation of Frizzled regulate cellular responsiveness to Wingless/Wnt. (2010)
  • Author(s): Mukai, A., et al.
  • Journal Title: The EMBO J. Volume: 29 Pages: 2114-2125
  • Peer Reviewed
• [Journal Article] Autophagy-dependent rhodopsin degradation prevents retinal degeneration in Drosophila. (2010)
  • Author(s): Midorikawa, R., et al.
  • Journal Title: J.Neurosci. Volume: 30 Pages: 10703-10719
  • Peer Reviewed
• [Journal Article] Spatial and temporal regulation of glycosylation during Drosophila eye development (2009)
  • Author(s): Yano, H., Yamamoto-Hino, M. and Goto, S.
  • Journal Title: Cell. Tissue Res. Volume: vol.336 Pages: 137-147
  • Peer Reviewed
• [Journal Article] Insight into the regulation of glycan synthesis in Drosophila chaoptin based on mass spectrometry (2009)
  • Author(s): Kanie, Y., Yamamoto-Hino, M., Karino, Y., Yokozawa, H., Nishihara, S., Ueda, R., Goto, S. and Kanie, O.
  • Journal Title: PLoS ONE Volume: vol.4 Issue: 5 Pages: e5434-e5434
  • DOI: 10.1371/journal.pone.0005434
  • Peer Reviewed
• [Journal Article] Insight into the regulation of glycan synthesis in Drosophila chaoptin based on mass spectrometry (2009)
  • Author(s): Kanie,Y., et. al.
  • Journal Title: PLoS ONE Vol.4
  • Peer Reviewed
• [Journal Article] Spatial and temporal regulation of glycosylation during Drosophilaeye development (2009)
  • Author(s): Yano,H., et. al.
  • Journal Title: Cell.Tissue Res. Vol.336 Pages: 137-147
  • Peer Reviewed
• [Journal Article] Membrane protein location-dependent regulation by PI3K(III)and Rabenosyn-5 in Drosophila wing cells (2009)
  • Author(s): Abe,M., et. al.
  • Journal Title: PLoS ONE Vol.4
  • Peer Reviewed
• [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析 (2011)
  • Author(s): 山本(日野)美紀、芝野孝子、岡野栄之、後藤聡
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: ハイブ長岡(新潟)
  • Year and Date: 2011-07-12
• [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity (2011)
  • Author(s): 山本(日野)美紀、芝野孝子、粟野若枝、村岡正敏、岡野栄之、後藤聡
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜パシフィコ(横浜)
• [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析 (2011)
  • Author(s): 山本(日野)美紀、岡野栄之、後藤聡
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉)
• [Presentation] 糖鎖関連遺伝子の大規模スクリーニング (2011)
  • Author(s): 山本(日野)美紀、蟹江善美、粟野若枝、木下聖子、西原祥子、岡野栄之、上田龍、蟹江治、後藤聡
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都)
• [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析 (2011)
  • Author(s): 山本(日野)美紀、粟野若枝、芝野孝子、岡野栄之、後藤聡
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都)
• [Presentation] Balanced ubiquitylation and deubiquitylation of Frizzled regulate cellular responsiveness to Wg/Wnt (2010)
  • Author(s): Mukai, A., et al.
  • Organizer: 16^<th> International Conference of the International Society of Differentiation
  • Place of Presentation: Nara (Nara-ken new public hall)
  • Year and Date: 2010-10-15
• [Presentation] Identification of neural specific glycosylation regulators using functional genomics (2010)
  • Author(s): Yamamoto-Hino, M., et al.
  • Organizer: 25^<th> International Carbohydrate Symposium
  • Place of Presentation: Chiba (Makuhari-messe)
  • Year and Date: 2010-08-01
• [Presentation] Balanced ubiquitylation and deubiquitylation of Frizzled regulate cellular responsiveness to Wingless/Wnt (2009)
  • Author(s): Mukai,A., et. al.
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] ユビキチン化、脱ユビキチン化によるFrizzledを介したWingless/Wntシグナリングの制御 (2009)
  • Author(s): 向井明子, 他
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-04
• [Presentation] ショウジョウバエを用いた糖修飾関連遺伝子のスクリーニング (2009)
  • Author(s): 山本(日野)美紀, 他
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-03
• [Book] NY, Structure, Function and Formation of Glycans in Drosophila, Glycans : Biochemistry, Characterization and Applications. (2012)
  • Author(s): Yamamoto-Hino, M., Okano, H., Kanie, O. and Goto, S.
  • Publisher: Nova Science publishers, Inc.
• [Book] Structure, Function and Formation of Glycans in Drosophila. In "Glycans : Biochemistry, Characterization and Applications." (2012)
  • Author(s): Yamamoto-Hino, M., Okano, H., Kanie, O., Goto, S.
  • Publisher: Nova Science publishers, Inc., NY