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Learning molecular design for acquired induced folding from the affinity maturation of antibodies.

Research Project

Project/Area Number 21570176
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

FURUKAWA Koji  独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (00297631)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsフォールディング / 抗体 / 蛋白質
Research Abstract

In this study, the first priority was construction of 1024 mutant phages. At the end of the 2010 fiscal year, we had almost completed the construction. Unfortunately, however, we were seriously damaged by the East Japan Great Earthquake. We lost a large number of the phage clones and expression vectors for the mutants. We are now making every effort to recover the lost clones and constructs. On the other hand, we had finished physicochemical analyses on the several important mutants. In addition, we had made great advances in technology necessary for the progress of this study(e. g.: improvement phage display, protein expression, and repertoire analysis). These have significant usefulness and are widely applicable.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (8 results)

All 2011 2010 2009

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (2 results)

  • [Journal Article] Conformational analysis of human serum albumin and its non-enzymatic glycation products using monoclonal antibodies2011

    • Author(s)
      Keigo Saito, Kuniko Hamano, Masatoshi Nakagawa, Keiko Yugawa, Jin Muraoka, Hiroyoshi Kuba, Koji Furukawa, Takachika Azuma
    • Journal Title

      J. Biochem

      Volume: 149 Issue: 5 Pages: 569-580

    • DOI

      10.1093/jb/mvr007

    • NAID

      10030585327

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conformational analysis of human serum albumin and its non-enzymatic glycation products using monoclonal antibodies.2011

    • Author(s)
      Saito K, Hamano K, Nakagawa M, Yugawa K, Muraoka J, Kuba H, Furukawa K, Azuma T
    • Journal Title

      J.Biochem.

      Volume: 149 Pages: 569-580

    • NAID

      10030585327

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Augmented antibody response with premature germinal center regression in CD40L-transgenic mice2010

    • Author(s)
      Yusuke Kishi, Yuichi Aiba, Tetsuya Higuchi, Koji Furukawa, Takeshi Tokuhisa, Toshitada Takemori, Takeshi Tsubata
    • Journal Title

      J. Immunol

      Volume: 185 Issue: 1 Pages: 211-219

    • DOI

      10.4049/jimmunol.0901694

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Augmented antibody response with premature germinal center regression in CD40L- transgenic mice.2010

    • Author(s)
      Kishi Y, Aiba Y, Higuchi T, Furukawa K, Tokuhisa T, Takemori T, Tsubata T
    • Journal Title

      J.Immunol.

      Volume: 185 Pages: 211-219

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An optimized procedure for efficient phage display of antibody fragments with a low folding efficiency2009

    • Author(s)
      Hiroyoshi Kuba, Koji Furukawa
    • Journal Title

      Protein Expr. Purif

      Volume: 65 Issue: 2 Pages: 148-153

    • DOI

      10.1016/j.pep.2009.01.016

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An optimized procedure for efficient phage display of antibody fragments with a low folding efficiency.2009

    • Author(s)
      Kuba H., Furukawa K.
    • Journal Title

      Protein Expr.Purif. 65

      Pages: 148-153

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Memory B cells generate the VH gene repertoire in the secondary response, distinct from that found in the primary antibody response2010

    • Author(s)
      Tomohiro Kaji, Koji Furukawa, Michiko Shimoda, Toshitada Takemori
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] 血清アルブミンの高次構造変化を識別する抗体の分子プローブとしての熱力学的評価2009

    • Author(s)
      齊藤桂吾、濱野邦子、久芳弘義、古川功治、東隆親
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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