Genetic analysis of dopamine signaling that regulates avoidance behavior in the nematode C. elegans

• Project/Area Number: 21570187
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: KIMURA Kotaro 大阪大学, 大学院・理学研究科, 特任准教授 (20370116)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子間相互作用 / 神経機能の可塑性 / 線虫C.elegans / ドーパミン / ノルアドレナリン / 神経機能 / 可塑性 / 分子遺伝学的解析

Research Abstract

Avoidance behavior of the nematode C. elegans to 2-nonanone is significantly enhanced, rather than reduced, after 1 hr-preexposure to the odor. In this project, we found that the D2-like dopamine receptor DOP-3 is required in a couple of interneurons RIC to regulate the enhancement to repulsive odor 2-nonanone in the animals. Our result may shed light on molecular mechanism of dopamine signaling that plays significant roles in regulating locomotion, emotion and learning in mammalian brain.

Research Products

• [Journal Article] A novel and conserved protein AHO-3 is required for thermotactic plasticity associated with feeding states in Caenorhabditis elegans. (2012)
  • Author(s): Nishio N, Mohri祐hiomi A, Nishida Y, Hiramatsu N, Kodama-Namba E, Kimura KD, Kuhara A, Mori I.
  • Journal Title: Genes to Cells Volume: 17 Pages: 365-386
  • Peer Reviewed
• [Journal Article] The C.elegans DAF-2 insulin-like receptor is abundantly expressed in the nervous system and regulated by nutritional status (2012)
  • Author(s): 木村幸太郎
  • Journal Title: Cold Spring Harbor Symposia on Quantitative Biology Volume: (印刷中)(未定) Issue: 0 Pages: 113-120
  • DOI: 10.1101/sqb.2011.76.010660
• [Journal Article] Ultradian rhythm in the intestine of Caenorhabditis elegans is controlled by the C-terminal region of the FLR-1 ion channel and the hydrophobic domain of the FLR-4 protein kinase. (2011)
  • Author(s): Kobayashi Y, Kimura KD, Katsura I.
  • Journal Title: Genes to Cells Volume: 16 Pages: 565-575
  • Peer Reviewed
• [Journal Article] Ultradian rhythm in the intestine of Caenorhabditis elegans is controlled by the C-terminal region of the FLR-1 ion channel and the hydrophobic domain of the FLR-4 protein kinase (2011)
  • Author(s): 小林百合、木村幸太郎
  • Journal Title: Genes to Cells Volume: 16 Issue: 5 Pages: 565-575
  • DOI: 10.1111/j.1365-2443.2011.01508.x
  • Peer Reviewed
• [Journal Article] Enhancement of Odor-avoidance regulated by Dopamine Signaling in Caenorhabditis elegans (2010)
  • Author(s): Kimura KD, Fujita K, Katsura I.
  • Journal Title: The Journal of Neuroscience Volume: 30 Pages: 16365-16375
  • Peer Reviewed
• [Journal Article] Enhancement of odor-avoidance regulated by dopmaine signaling in Caenorhabditis elegans. (2010)
  • Author(s): Kimura KD, Fujita K, Katsura I
  • Journal Title: The Journal of Neuroscience Volume: 30 Pages: 16365-16475
  • Peer Reviewed
• [Journal Article] FLR-2, the glycoprotein hormone alpha subunit, is involved in the neural control of intestinal functions in Caenorhabditis elegans. (2009)
  • Author(s): Oishi A, Gengyo-Ando K, Mitani S, Mohri祐hiomi A, Kimura KD, Ishihara T, Katsura I.
  • Journal Title: Genes to cells Volume: 14 Pages: 1141-1154
  • Peer Reviewed
• [Journal Article] FLR-2, the glycoprotein hormone alpha subunit, is involved in the neural control of intestinal functions in Caenorhabditis elegans (2009)
  • Author(s): Oishi A, Gengyo-Ando K, Mitani S, Mohri-Shiomi A, Kimura KD, Ishihara T, Katsura I
  • Journal Title: Genes to Cells 14 Pages: 1141-1154
  • Peer Reviewed
• [Journal Article] A simple optogenetic system for behavioral analysis of freely moving small animals.
  • Author(s): Kawazoe K, Yawo H, Kimura KD.
  • Journal Title: Neuroscience Research Volume: (in press)
  • Peer Reviewed
• [Presentation] 線虫の匂い忌避学習を制御するドーパミンの作用メカニズム (2012)
  • Author(s): 木村幸太郎
  • Organizer: 京都大学霊長類研究所共同利用研究会
  • Place of Presentation: 愛知県犬山市・京都大学霊長類研究所
  • Year and Date: 2012-03-15
• [Presentation] Repulsive odor learning regulated by dopamine signaling in worms (2011)
  • Author(s): 木村幸太郎
  • Organizer: 包括脳ワークショップ・シンポジウム「報酬系・罰系とモノアミンシグナル伝達
  • Place of Presentation: 兵庫県神戸市・神戸国際会議場
  • Year and Date: 2011-08-22
• [Presentation] An integrative and quantitative analysis of dopamine-dependent odor avoidance behavior of the nematode C. elegans (2011)
  • Author(s): 木村幸太郎
  • Organizer: 第8回国際比較生理生化学会議
  • Place of Presentation: 愛知県名古屋市・名古屋国際会議場
  • Year and Date: 2011-06-02
• [Presentation] An integrative and quantitative analysis of dopamine-dependent odor avoidance behavior of the nematode C.elegans (2011)
  • Author(s): 木村幸太郎
  • Organizer: 第8回国際比較生理生化学会議
  • Place of Presentation: 愛知県名古屋市名古屋国際会議場(招待講演)
  • Year and Date: 2011-06-02
• [Presentation] Enhancement of odor avoidance regulated by dopmaine signaling in the C.elegans (2010)
  • Author(s): 木村幸太郎
  • Organizer: 国立遺伝学研究所Biological Symposium
  • Place of Presentation: 国立遺伝学研究所(静岡)(招待講演)
  • Year and Date: 2010-12-22
• [Presentation] 忌避行動の学習を制御する神経回路～線虫C.elegansをモデルとして～ (2010)
  • Author(s): 木村幸太郎
  • Organizer: 立命館大学シンポジウム「感覚器と神経回路のサイエンス」
  • Place of Presentation: 立命館大学(滋賀)(招待講演)
  • Year and Date: 2010-09-17
• [Presentation] 忌避行動の学習を制御する神経回路～線虫C. elegansをモデルとして～ (2010)
  • Author(s): 木村幸太郎
  • Organizer: 立命館大学シンポジウム
  • Place of Presentation: 滋賀県草津市・立命館大学びわこ・くさつキャンパス
  • Year and Date: 2010-09-10
• [Presentation] Enhancement of odor avoidance regulated by dopamine signaling in the nematode C. elegans (2010)
  • Author(s): 木村幸太郎
  • Organizer: 第87回日本生理学会大会・シンポジウム
  • Place of Presentation: 岩手県盛岡市・盛岡市民文化ホール
  • Year and Date: 2010-05-21
• [Presentation] Enhancement of odor avoidance regulated by dopmaine signaling in thenematode C.elegans (2010)
  • Author(s): 木村幸太郎
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手)(招待講演)
  • Year and Date: 2010-05-21
• [Presentation] Enhancement of odor avoidance is regulated by dopamine signaling in the nematode C. elegans (2009)
  • Author(s): 木村幸太郎
  • Organizer: 第7回国際シンポジウム「味覚嗅覚の分子神経機構」
  • Place of Presentation: 九州大学 (福岡)
  • Year and Date: 2009-11-04
• [Presentation] Enhancement of odor avoidance is regulated by dopamine signaling in the nematode C. elegans (2009)
  • Author(s): 木村幸太郎
  • Organizer: 第7回国際シンポジウム
  • Place of Presentation: 福岡県福岡市・九州大学
  • Year and Date: 2009-11-03