Molecular dissection of meiotic endocytosis

• Project/Area Number: 21570202
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka City University
• Principal Investigator: NAKAMURA Taro 大阪市立大学, 大学院・理学研究科, 教授 (30291082)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: GFP / エンドサイトーシス / 分裂酵母 / 減数分裂 / 膜輸送 / シンタキシン / SNARE / 酵母 / 胞子形成

Research Abstract

The plasma membrane syntaxin 1 ortholog Psy1 is internalized and changes its localization to the forespore membrane during meiosis. The aim of this study is to address the molecular mechanism of this meiotic endocytosis of Psy1. An arrestin-like protein M

Research Products

• [Journal Article] Remarkably simple sequence requirement of the M-factor pheromone ofSchizosaccharomyces pombe (2012)
  • Author(s): Seike T, Yamagishi Y, Iio H, *Nakamura T., Shimoda C
  • Journal Title: Genetics Volume: 191 Pages: 815-822
  • Peer Reviewed
• [Journal Article] Remarkably Simple Sequence Requirement of the M-Factor Pheromone of Schizosaccharomyces pombe (2012)
  • Author(s): Seike T, Yamagishi Y, Iio H, Nakamura T, Shimoda C.
  • Journal Title: Genetics Volume: 191(3) Pages: 815-825
  • Peer Reviewed
• [Journal Article] Endocytosis is essential for dynamic translocation of a syntaxin 1 ortholog during fission yeast meiosis (2011)
  • Author(s): Kashiwazaki J, Yamasaki Y, Itadani A, Teraguchi E, Maeda Y, Shimoda C, Nakamura T
  • Journal Title: Mol Biol Cell Volume: 2219 Pages: 3658-3670
  • Peer Reviewed
• [Journal Article] The fission yeast pleckstrin homology domain protein Spo7 is essential for initiation of forespore membrane assembly and spore morphogenesis. (2011)
  • Author(s): Nakamura-Kubo M, Hirata A, Shimoda C, Nakamura T
  • Journal Title: Mol Biol Cell Volume: 22 Pages: 3442-3455
  • Peer Reviewed
• [Journal Article] Fission yeast calmodulin, Cam1, plays a crucial role in sporulation by recruiting and stabilizing the spindle pole body components responsible for assembly of the forespore membrane. (2010)
  • Author(s): Itadani A, Nakamura T, Hirata A, Shimoda C.
  • Journal Title: Eukaryot. Cell Volume: 9 Pages: 1925-1935
  • Peer Reviewed
• [Journal Article] Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma-membrane proteins in Schizosaccharomyces pombe (2010)
  • Author(s): Nakase M, Tani M, Morita T, Kitamoto HK, Kashiwazaki J, Nakamura T, Hosomi A, Tanaka N, Takegawa K
  • Journal Title: J. Cell Sci Volume: 123 Pages: 1578-1587
  • Peer Reviewed
• [Journal Article] Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast. (2010)
  • Author(s): Onishi M, Koga T, Hirata A, Nakamura T, Asakawa H, Shimoda C, Bahler J, Wu JQ, Takegawa K, Tachikawa H, Pringle JR, Fukui Y
  • Journal Title: Mol. Cell Biol. Volume: 8 Pages: 2057-2074
  • Peer Reviewed
• [Journal Article] NBRP databases: databases of biological resources in Japan. (2010)
  • Author(s): Yamazaki Y, Akashi R, Banno Y, Endo T, Ezura H, Fukami-Kobayashi K, Inaba K, Isa T, Kamei K, Kasai F, Kobayashi M, Kurata N, Kusaba M, Matuzawa T, Mitani S, Nakamura Y, Nakatsuji N, Naruse K, Niki H, Nitasaka E, Obata Y, Okamoto H, Okuma M, Sato K, Serika
  • Journal Title: Nucleic Acids Res Volume: D26-30
  • NAID: 120005398246
  • Peer Reviewed
• [Journal Article] Schizosaccharomyces pombe calmodulin, Cam1, plays a crucial role in sporulation by recruiting and stabilizing the spindle pole body components responsible for assembly of the forespore membrane (2010)
  • Author(s): Itadani, A., Nakamura, T., Hirata, A., Shimoda, C.
  • Journal Title: Eukaryotic Cell Volume: 9 Pages: 1925-1935
  • Peer Reviewed
• [Journal Article] Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma-membrane proteins in Schizosaccharomyces pombe (2010)
  • Author(s): Nakase, M., Tani, M., Morita, T., Kitamoto, HK., Kashiwazaki, J., Nakamura, T., Hosomi, A., Tanaka, N., Takegawa, K.
  • Journal Title: Journal of Cell Science Volume: 123 Pages: 1578-1587
  • Peer Reviewed
• [Journal Article] Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast (2010)
  • Author(s): Onishi, M., Koga, T., Hirata, A., Nakamura, T., Asakawa, H., Shimoda, C., Bahler, J., Wu, JQ., Takegawa, K., Tachikawa, H., Pringle, JR., Fukui, Y
  • Journal Title: Molecular and Cellular Biology Volume: 8 Pages: 2057-2074
  • Peer Reviewed
• [Journal Article] NBRP databases : databases of biological resources in Japan (2010)
  • Author(s): Yamazaki, Y., Akashi, R., Banno, Y., Endo, T., Ezura H, Fukami-Kobayashi, K., Inaba, K. Isa, T., Kamei, K., Kasai, F., Kobayashi, M., Kurata, N., Kusaba, M., Matuzawa, T., Mitani, S., Nakamura, T., et al.
  • Journal Title: Nucleic Acids Research Volume: 38
  • NAID: 120005398246
• [Journal Article] Autophagy in the fission yeast Schizosaccharomyces pombe (2010)
  • Author(s): Mukaiyama, H., Nakase, M., Nakamura, T., Kakinuma, Y., Takegawa, K
  • Journal Title: FEBS Letters Volume: 584 Pages: 1327-1334
• [Journal Article] Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation (2009)
  • Author(s): Mukaiyama H, Kajiwara S, Hosomi A, Giga-Hama Y, Tanaka N, Nakamura T, Takegawa K
  • Journal Title: Microbiology Volume: 155 Pages: 3816-3826
  • Peer Reviewed
• [Journal Article] Ectopic overproduction of a meiosis-specific transcription factor induces assembly of prospore-like membranous compartments in vegetative cells of fission yeast (2009)
  • Author(s): Nakase Y, Hirata A, Shimoda C, Nakamura T
  • Journal Title: Genetics Volume: 183 Pages: 1195-1199
  • Peer Reviewed
• [Journal Article] Two fission yeast rab7 homologs, ypt7 and ypt71, play antagonistic roles in the regulation of vacuolar morphology (2009)
  • Author(s): Kashiwazaki J, Iwaki T, Takegawa K, Shimoda C, Nakamura T.
  • Journal Title: Traffic Volume: 10 Pages: 912-924
  • Peer Reviewed
• [Journal Article] Ectopic overproduction of a meiosis-specific transcription factor induces assembly of prospore-like membranous compartments in vegetative cells of fission yeast. (2009)
  • Author(s): Nakase Y, Hirata A, Shimoda C, ^*Nakamura T.
  • Journal Title: Genetics 183 Pages: 1195-1199
  • Peer Reviewed
• [Journal Article] Two fission yeast rab7 homologs, ypt7 and ypt71, play antagonistic roles in the regulation of vacuolar morphology (2009)
  • Author(s): Kashiwazaki J, Iwaki T, Takegawa K, Shimoda C, ^*Nakamura T.
  • Journal Title: Traffic 10 Pages: 912-924
  • Peer Reviewed
• [Journal Article] Schizosaccharomyces pombe syntaxin 1 homologue, Psy1, is essential for development of the forespore membrane (2009)
  • Author(s): Maeda Y, Kashiwazaki J, Shimoda C, ^*Nakamura T.
  • Journal Title: Biosci Biotechnol Biochem. 73 Pages: 339-345
  • Peer Reviewed
• [Presentation] 分裂酵母シンタキシン1の胞子形成特異的なエンドサイトーシスの分子メカニズム (2013)
  • Author(s): 東北大学川内北キャンパス
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内北キャンパス
• [Presentation] 分裂酵母シンタキシン1の胞子形成特異的なエンドサイトーシスの分子メカニズム (2013)
  • Author(s): 山﨑百合子、寺口絵理香、中村太郎(大阪市大・院理・生物 地球)
  • Organizer: 日本農芸化学会
  • Place of Presentation: 仙台 東北大学川内北キャンパス
• [Presentation] 分裂酵母の胞子形成の開始に必要なRab-GEFカスケードの解析 (2013)
  • Author(s): 今田一姫、中村太郎（大阪市大・院理・生物地球）
  • Organizer: 日本農芸化学会
  • Place of Presentation: 仙台 東北大学川内北キャンパス
• [Presentation] 分裂酵母シンタキシン1の胞子形成時エンドサイトーシスの分子機構 (2012)
  • Author(s): 山崎百合子、寺口絵理香、中村太郎
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] 分裂酵母シンタキシン1の胞子形成時エンドサイトーシスの分子機構 (2012)
  • Author(s): 山﨑百合子、寺口絵理香、中村太郎(大阪市大・院理・生物 地球)
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡 福岡国際会議場
• [Presentation] Rab cascade for exocytosis plays a key role in the initiation of spore formation in fission yrast (2012)
  • Author(s): 今田一姫、寺口絵理香、中村太郎（大阪市大・院理・生物地球）
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 分裂酵母シンタキシンの減数分裂特異的局在変化の分子メカニズム (2011)
  • Author(s): 柏崎隼、寺口絵里香、山崎百合子、下田親、中村太郎
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2011-01-09
• [Presentation] メンブレントラフィックと胞子形成 分裂酵母の胞子形成欠損株の解析から (2011)
  • Author(s): 山崎百合子、寺口絵理香、中村太郎
  • Organizer: 第44回酵母遺伝学フォーラム
  • Place of Presentation: 九州大学医学部百年講堂
• [Presentation] 胞子形成特異的な分裂酵母シンタキシン1 オルソログのエンドサイトーシスの解析 (2011)
  • Author(s): 山崎百合子、寺口絵理香、中村太郎
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学
• [Presentation] 分裂酵母の完全長cDNAの整備とバイオリソースとしての利用 (2010)
  • Author(s): 中村太郎
  • Organizer: 第19回酵母合同シンポジウム
  • Place of Presentation: 東京大学(招待講演)
  • Year and Date: 2010-06-25
• [Presentation] 胞子形成時のおける分裂酵母シンタキシン1オルソログの局在変化の分子メカニズム (2010)
  • Author(s): 山崎百合子、寺口絵理香、下田 親、中村太郎
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 減数分裂における分裂酵母シンタキシンの局在変化の分子メカニズム (2010)
  • Author(s): 寺口絵理香、柏崎 隼、下田 親、中村太郎
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪国際会議場
• [Presentation] 胞子形成時のおける分裂酵母シンタキシン1オルソログの局在変化の分子メカニズム (2010)
  • Author(s): 山崎百合子、寺口絵理香、下田親、中村太郎
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 分裂酵母の胞子形成過程に関する新しい因子の取得と解析 (2010)
  • Author(s): 今田一姫、寺口絵理香、下田親、中村太郎
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 分裂酵母における新しい胞子形成関連遺伝子の単離と解析 (2010)
  • Author(s): 今田一姫、寺口絵理香、下田親、中村太郎
  • Organizer: 第43回酵母遺伝学フォーラム
  • Place of Presentation: ならまちセンター
• [Presentation] Endocytosis during meiosis (2009)
  • Author(s): Taro Nakamura
  • Organizer: Fifth International Fission Yeast Meeting
  • Place of Presentation: Tokyo
  • Year and Date: 2009-10-28
• [Presentation] 分裂酵母の配偶子形成におけるメンブレントラフィック (2009)
  • Author(s): 中村太郎
  • Organizer: バイオリソースシンポジウム「KYOTO BRAND高品質遺伝資源の開発」
  • Place of Presentation: 京都
  • Year and Date: 2009-10-23
• [Presentation] 分裂酵母ゲノムDNAクローンの包括的解析と整備 (2009)
  • Author(s): 中村太郎、成田貴則、山崎由紀子、小原雄治、下田親
  • Organizer: 第42回 酵母遺伝学フォーラム
  • Place of Presentation: 筑波
  • Year and Date: 2009-07-28
• [Presentation] 胞子形成をモデルとした細胞膜新生メカニズムの解明 (2009)
  • Author(s): 中村太郎
  • Organizer: 第16回日本微生物資源学会 シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2009-06-26
• [Presentation] Endocytosis during meiosis (2009)
  • Author(s): Jun Kashiwazaki, Erika Teraguchi, Akiko Itadani, Chikashi Shimoda, and Taro Nakamura
  • Organizer: International fission yeast meeting 2009
  • Place of Presentation: 東京
• [Presentation] Isolation and characterization of fission yeast mutants defective in relocalization of syntaxin 1 homolog during meiosis (2009)
  • Author(s): Erika Teraguchi, Jun Kashiwazaki, Chikashi Shimoda and Taro Nakamura
  • Organizer: International fission yeast meeting 2009
  • Place of Presentation: 東京
• [Presentation] Endocytosis of fission yeast syntaxin 1 orthologue during meiosis
  • Author(s): 山崎百合子、寺口絵理香、中村太郎
  • Organizer: 第63回日本細胞生物学学会
  • Place of Presentation: 北海道大学 クラーク会館
• [Remarks]
  • URL: http://www.sci.osaka-cu.ac.jp/biol/cbiol/pombe/pombe_J.htm
• [Remarks]
  • URL: http://www.sci.osaka-cu.ac.jp/biol/cbiol/pombe/pombe_J.htm