Molecular mechanism of crinophagy

Research Project

Project/Area Number	21570206
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Cell biology
Research Institution	Nagahama Institute of Bio-Science and Technology
Principal Investigator	YAMAMOTO Akitsugu 長浜バイオ大学, バイオサイエンス学部, 教授 (30174775)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	細胞性粘菌 / Dictyostelium discoideum / クリノファジー / PSV / リソソーム / エンドソーム / 電子顕微鏡 / プロテオミックス / 分泌顆粒 / プロテオミック / バフィロマイシンA1
Research Abstract	During dedifferentiation process of prespore cells of cellular slime mold Dictyostelium discoideum, it was revealed by electron microscopic observation that PSVs ; secretory granules specifically present in prespore cells sre degraded simultaneously by crinophagy in which PSVs are directly fused with lysosomes. In crinophagy of PSVs, it was found that PSVs fuse with endosomes as well as with lysosomes, suggesting that endosomes are deeply concerned with crinophagy.