Evolutionary analysis of duplicated genes enhancer in teleost lineage by using fugu embryos.

• Project/Area Number: 21570218
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: KUROKAWA Daisuke 東京大学, 大学院・理学系研究科, 助教 (40342779)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Otx / ゲノム / フグ / 発生進化 / ゲノム重複

Research Abstract

We compared the organizer enhancer activities between Fugu Otx2a and Otx2b which are duplicated by teleost specific genome duplication. For this purpose, we adapted the successful induction of reporter gene DNA and morpholino antisense oligo molecules into the fertilized eggs of Takifugu niphobles(Kusafugu). The feasibility to perform transgenesis is an important step to establish T. niphobles as new model in evolution and developmental research.

Research Products

• [Journal Article] Gbx2directly restricts Otx2 expression to forebrain-midbrain, competing with ClassIII POU factors (2012)
  • Author(s): Fumitaka Inoue, Daisuke Kurokawa, Maiko Takahashi, Shinichi Aizawa
  • Journal Title: Mol. Cell BIol Volume: (in press) Issue: 13 Pages: 2618-2627
  • DOI: 10.1128/mcb.00083-12
  • Peer Reviewed
• [Journal Article] Nervous system disruption and concomitant behavioral abnormality in early-hatched pufferfish larvae exposed to heavy oil (2012)
  • Author(s): Masahumi Kawaguchi, Yuki Sugahara, Tomoe Watanabe, Kouta Irie, Minoru Ishida, Daisuke Kurokawa, Shin-Ichi Kitamura, Itsuki C. Handoh, Kei Nakayama, Yasunori Murakami.
  • Journal Title: Enviromental Science and Pollution Research Volume: (in press) Issue: 7 Pages: 2488-2497
  • DOI: 10.1007/s11356-012-0833-0
  • Peer Reviewed
• [Journal Article] A lineage specific enhancer drives Otx2 expression in teleost organizer tissues (2012)
  • Author(s): Daisuke Kurokawa, Tomomi Ohmura, Koji Akasaka, Shinichi Aizawa
  • Journal Title: Mechanisms of Development Volume: 128 Issue: 11-12 Pages: 653-661
  • DOI: 10.1016/j.mod.2011.11.001
  • Peer Reviewed
• [Journal Article] Nervous system disruption and concomitant behavioral abnormality in early-hatched pufferfish larvae exposed to heavy oil (2012)
  • Author(s): 川口将史, 他10名
  • Journal Title: Enviromental Science and Pollution Research Volume: (In press)
  • Peer Reviewed
• [Journal Article] Acquisition of glial cells missing 2 enhancers contributes to a diversity of ionocytes in zebrafish (2011)
  • Author(s): Shono T, Kurokawa D, Miyake T, Okabe M
  • Journal Title: PLoS ONE Volume: 6(8) Issue: 8 Pages: e23746-e23746
  • DOI: 10.1371/journal.pone.0023746
  • Peer Reviewed
• [Journal Article] Evolutionary origin of the Otx2 enhancer for its expression in visceral endoderm (2010)
  • Author(s): Daisuke Kurokawa*, Tomomi Ohmura*, Hajime Ogino, Masaki Takeuchi, Ai Inoue, Fumitaka, Inoue, Yoko Suda and Shinichi Aizawa
  • Journal Title: Developmental Biology Volume: 342 Issue: 1 Pages: 110-20
  • DOI: 10.1016/j.ydbio.2010.03.013
  • Peer Reviewed
• [Journal Article] Evolutionary origin of the Otx2 enhancer for its expression in visceral endoderm. (2010)
  • Author(s): 黒川大輔, 他7名
  • Journal Title: Developmental Biology Volume: 342 Pages: 110-120
  • Peer Reviewed
• [Journal Article] Otx2 and Otx1 protect diencephalon and mesencephalon from caudalization into metencephalon during early brain regionalization (2010)
  • Author(s): 黒川大輔, 他5名
  • Journal Title: Developmental Biology Volume: 347 Pages: 392-403
  • Peer Reviewed
• [Journal Article] Evolutionary origin of the Otx2 enhancer for its expression in visceral endoderm. (2010)
  • Author(s): 黒川大輔, 他7名
  • Journal Title: Developmental Biology (In Press)
  • Peer Reviewed
• [Presentation] 一年魚の発生 (2011)
  • Author(s): 黒川大輔
  • Organizer: 日本発生生物学会秋期シンポジウム2011
  • Place of Presentation: 愛知県・岡崎
  • Year and Date: 2011-12-19
• [Presentation] トランスジェニッククサフグを用いた真骨魚類で重複したOtx2遺伝子の転写調節の解析 (2011)
  • Author(s): 黒川大輔,大村朋美,赤坂甲治,相沢慎一
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 北海道・旭川
  • Year and Date: 2011-09-21
• [Presentation] トランスジェニッククサフグを用いた真骨魚類で重複したOtx2遺伝子の転写調節の解析 (2011)
  • Author(s): 黒川大輔, 他4名
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 北海道・旭川市
  • Year and Date: 2011-09-21
• [Presentation] Evolutionary origin of Otx2 enhancer for its expression in visceral endoderm (2010)
  • Author(s): Daisuke Kurokawa, Tomomi Ohmura, HajimeOgino, Masaki Takeuchi, Fumitaka Inoue, Yoko Suda, Kazuki Nakao, Shinichi Aizawa
  • Organizer: Society of for Developmental Biology 69th Annual meeting
  • Place of Presentation: 米国アルバカーキ
  • Year and Date: 2010-08-07
• [Presentation] Evolutionary origin of Otx2 enhancer for its expression in visceral elldoderm (2010)
  • Author(s): Daisuke Kurokawa, 他8名
  • Organizer: Society of for Developmental Biology 69^<th> Annual meeting.
  • Place of Presentation: アルバカーキ 米国
  • Year and Date: 2010-08-07
• [Presentation] Evolutionary origin of the Otx2 enhancer for its expression in visceral endoderm (2010)
  • Author(s): Daisuke Kurokawa, Tomomi Ohmura, Hajime Ogino, Masaki Takeuchi, Fumitaka Inoue, Yoko Suda, Shinichi Aizawa
  • Organizer: 2^<ND> Joint meeting of the French and Japanese Societies for Developmental Biology
  • Place of Presentation: フランスパリ
  • Year and Date: 2010-05-27
• [Presentation] Evolutionary Origin of Otx2 enhancer for its expression in visceral endoderm (2009)
  • Author(s): Daisuke Kurokawa, Tomomi Ohmura, Hajime Ogino, Masaki Takeuchi, Ai Inoue, Yoko Suda, Shinichi Aizawa
  • Organizer: 42^<nd> Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 新潟県・新潟市
  • Year and Date: 2009-05-28
• [Presentation] Evolutionary Origin of Otx2 expression for its expression in visceral endoderm (2009)
  • Author(s): 黒川大輔, 他6名
  • Organizer: 42nd Annual meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 朱鷺メッセ(新潟県)
  • Year and Date: 2009-05-28
• [Remarks]
  • URL: http://www.mmbs.s.u-tokyo.ac.jp/
• [Remarks]
  • URL: http://www.mmbs.s.u-tokyo.ac.jp/jp/research/individuals.html#indi10