Isolation and analyses of novel regulatory factors in differentiation of blood islands of frog embryo

• Project/Area Number: 21570220
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Niigata University
• Principal Investigator: MAENO Mitsugu 新潟大学, 自然科学系, 准教授 (10190315)
• Co-Investigator(Kenkyū-buntansha): KATO Takashi 早稲田大学, 教育・総合科学学術院, 教授 (80350388)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 器官形成 / 脊椎動物 / 骨髄球 / ゼノパス / BMP / Wnt / 転写制御 / モルフォリノ / 起源

Research Abstract

The physiological and biochemical properties of Val and Rdd proteins found in the Xenopus embryo have been investigated. We examined the biological activity and biochemical property of the various deletion mutants of Val. We also examined the association of Rdd proteins in the vascular system in the developing embryo. Furthermore, two distinct origins and differential regulations of primitive myeloid cells appeared in the tailbud embryo have been analyzed.

Research Products

• [Journal Article] Distinct mechanisms control the timing of differentiation of two myeloid populations in Xenopus ventral blood islands (2012)
  • Author(s): Maeno, M., Komiyama, K., Matsuzaki, Y., Hosoya, J., Kurihara, S., Sakata, H., Izutsu, Y.
  • Journal Title: Dev. Growth Differ Volume: 54 Pages: 187-201
  • NAID: 10030405616
  • Peer Reviewed
• [Journal Article] Distinct mechanisms control the timing of differentiation of two myeloid populations in Xenopus ventral blood islands (2012)
  • Author(s): Maeno, M., Komiyama, K., Matsuzaki, Y., et al.
  • Journal Title: Develop.Growth Differ. Volume: Vol.54 Issue: 2 Pages: 187-201
  • DOI: 10.1111/j.1440-169x.2011.01321.x
  • NAID: 10030405616
  • Peer Reviewed
• [Journal Article] A novel approach for a toxicity prediction model of environmental pollutants by using a quantitative structure-activity relationship method based on toxicogenomics (2011)
  • Author(s): Hosoya, J., Tamura, K., Muraki, N., Okumura, H., Ito, T., Maeno, M.
  • Journal Title: ISRN Toxicology Article Pages: 9-9
  • Peer Reviewed
• [Journal Article] Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis (2011)
  • Author(s): Nogawa-Kosaka, N., Sugai, T., Nagasawa, K., Tanizaki, Y., Meguro, M, Aizawa, Y., Maekawa, S., Adachi, M., Kuroki, R. and Kato, T
  • Journal Title: J Exp Biol. Volume: 214 Pages: 921-927
  • Peer Reviewed
• [Journal Article] A novel approach for a toxicity prediction model of environmental pollutants by using a quantitative structure-activity relationship method based on toxicogenomics (2011)
  • Author(s): Hosoya, J., Tamura, K., Muraki, N., et al.
  • Journal Title: ISRN Toxicology Volume: Vol.2011 Pages: 9-9
  • DOI: 10.5402/2011/515724
  • Peer Reviewed
• [Journal Article] Identification of erythroid progenitors induced by erythropoietic activity in Xenopus laevis. (2011)
  • Author(s): Nogawa-Kosaka N, Sugai T, Nagasawa K, et al.
  • Journal Title: J Exp Biol. Volume: 214(Pt 6) Pages: 921-927
  • Peer Reviewed
• [Journal Article] Involvement of Neptune in induction of the hatching gland and neural crest in the Xenopus embryo (2010)
  • Author(s): Kurauchi, T., Izutsu, Y. and Maeno, M.
  • Journal Title: Differentiation Volume: 4/5 Pages: 251-259
  • Peer Reviewed
• [Journal Article] Identification and expression of Ventrally associated leucine-zipper(VAL) in Xenopus embryo (2010)
  • Author(s): Saito, Y., Takahashi, Y., Izutsu, Y. and Maeno, M.
  • Journal Title: Int. J. Dev. Biol. Volume: 54 Pages: 203-208
  • Peer Reviewed
• [Journal Article] Structural and biological properties of erythropoietin in Xenopus laevis (2010)
  • Author(s): Nogawa-Kosaka, N., Hirose, T., Kosaka, N., Aizawa, Y., Nagasawa, K., Uehara, N., Miyazaki, H., Komatsu, N. and Kato, T.
  • Journal Title: Exp. Hematol Volume: 38 Pages: 363-372
  • Peer Reviewed
• [Journal Article] Identification and expression of Ventrally associated leucine-zipper (VAL) in Xenopus embryo. (2010)
  • Author(s): Saito, Y., et al.
  • Journal Title: Int.J.Dev.Biol. Volume: Vol.54 Pages: 203-208
  • Peer Reviewed
• [Journal Article] Involvement of Neptune in induction of the hatching gland and neural crest in the Xenopus embryo. (2010)
  • Author(s): Kurauchi.T., et al.
  • Journal Title: Differentiation Volume: Vol.4/5 Pages: 251-259
  • Peer Reviewed
• [Journal Article] Structural and biological properties of erythropoietin in Xenopus laevis. (2010)
  • Author(s): Nogawa-Kosaka N, Hirose T, Kosaka N., et al.
  • Journal Title: Exp.Hematol. Volume: Vol.38 Pages: 363-372
  • Peer Reviewed
• [Journal Article] Identification and expression of Ventrally associated leucine-zipper (VAL) in Xenopus embryo. (2010)
  • Author(s): Saito, Y., et al.
  • Journal Title: Int.J.Dev.Biol. Vol.54 Pages: 203-208
  • Peer Reviewed
• [Journal Article] Involvement of Neptune in induction of the hatching gland and neural crest in the Xenopus embryo. (2010)
  • Author(s): Kurauchi.T., et al.
  • Journal Title: Differentiation (印刷中)
  • Peer Reviewed
• [Journal Article] Structural and biological properties of erythropoietin in Xenopus laevis. (2010)
  • Author(s): Nogawa-Kosaka N, Hirose T, Kosaka N., et al.
  • Journal Title: Exp. Hematol. 38(印刷中)
  • Peer Reviewed
• [Journal Article] The keratin-related Ouroboros proteins function as immune antigens mediating tail regression in Xenopus metamorphosis (2009)
  • Author(s): Mukaigasa, K., Hanasaki, A., Maeno, M., Fujii, H., Hayashida, S., Itoh, M., Kobayashi, M., Tochinai, S., Hatta, M., Iwabuchi, K., Taira, M., Onoe, K. and Izutsu, Y.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 106 Pages: 18309-18314
  • Peer Reviewed
• [Journal Article] Involvement of AP-2rep in morphogenesis of the axial mesoderm in Xenopus embryo (2009)
  • Author(s): Saito, Y., Gotoh, M., Ujiie, Y., Izutsu, Y. and Maeno, M.
  • Journal Title: Cell Tissue Res. Volume: 335 Pages: 357-369
  • Peer Reviewed
• [Journal Article] Involvement of AP-2rep in morphogenesis of the axial mesoderm in Xenopus embryo. (2009)
  • Author(s): Saito, Y., et al.
  • Journal Title: Cell Tissue Res. Vol.335 Pages: 357-369
  • Peer Reviewed
• [Journal Article] The keratin-related Ouroboros proteins function as immune a ntigens mediating tail regression in Xenopus metamorphosis. (2009)
  • Author(s): Mukaigasa, K., et al.
  • Journal Title: Proc.Natl.Acad.Sci.USA Vol.106 Pages: 18309-18314
  • Peer Reviewed
• [Presentation] 造血研究の新展開をめざす~なぜカエルなの?なんて,もう訊かないで (2012)
  • Author(s): 加藤尚志
  • Organizer: 日本動物学会第64回関東支部大会公開講演会
  • Place of Presentation: 東邦大学,東京
  • Year and Date: 2012-03-17
• [Presentation] 造血研究の新展開をめざす～なぜカエルなの?なんて,もう訊かないで (2012)
  • Author(s): 加藤尚志
  • Organizer: 日本動物学会第64回関東支部大会公開講演会
  • Place of Presentation: 東京(東邦大学)(招待講演)
  • Year and Date: 2012-03-17
• [Presentation] アフリカツメガエル肝臓の赤血球造血に関わる接着分子の発現 (2011)
  • Author(s): 奥井武仁ほか
  • Organizer: 第36回日本比較内分泌学会大会
  • Place of Presentation: 東京都道府県会館
  • Year and Date: 2011-11-23
• [Presentation] アフリカツメガエル胚前方腹部血島における骨髄球分化制御 (2011)
  • Author(s): 坂田広之ほか
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 旭川大雪クリスタルホール
  • Year and Date: 2011-09-21
• [Presentation] アフリカツメガエルG-CSFの生物活性と特異性 (2011)
  • Author(s): 栗城遥ほか
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 旭川大雪クリスタルホール
  • Year and Date: 2011-09-21
• [Presentation] アフリカツメガエル胚前方腹部血島における骨髄球分化制御 (2011)
  • Author(s): 坂田広之, 松崎陽子, ほか
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川大雪クリスタルホール
  • Year and Date: 2011-09-21
• [Presentation] アフリカツメガエルG-CSFの生物活性と特異性 (2011)
  • Author(s): 栗城遥, 横田直樹, ほか
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川大雪クリスタルホール
  • Year and Date: 2011-09-21
• [Presentation] Expression and localizati on of rdd, a novel secretory protein, in Xenopus embryo (2011)
  • Author(s): 真島豊ほか
  • Organizer: 第44回日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-19
• [Presentation] Expression and localization of rdd, a novel secretory protein, in Xenopus embryo (2011)
  • Author(s): 真島豊, Jong-Chan Lim, ほか
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2011-05-19
• [Presentation] ツメガエル中胚葉分化パターンにかかわるNeptuneの予想外の働き (2010)
  • Author(s): 星野亨ほか
  • Organizer: 第81回日本動物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-24
• [Presentation] Xenopusの分泌蛋白質Rddは神経冠に由来する組織の分化に必要である (2010)
  • Author(s): 玉木理絵ほか
  • Organizer: 第81回日本動物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-24
• [Presentation] 成体アフリカツメガエル肝臓における赤血球前駆細胞の局在と貧血応答 (2010)
  • Author(s): 奥井武仁ほか
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜パシフィコ
  • Year and Date: 2010-09-24
• [Presentation] 低温曝露ツメガエルにおける肝臓での赤血球破壊とヘム分解酵素発現量の増大 (2010)
  • Author(s): 前川峻ほか
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-24
• [Presentation] ツメガエル中胚葉分化パターンにかかわるNeptuneの予想外の働き (2010)
  • Author(s): 星野亨, 倉内孝行, ほか
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-24
• [Presentation] Xenopusの分泌蛋白質Rddは神経冠に由来する組織の分化に必要である (2010)
  • Author(s): 玉木理絵, 真島豊, ほか
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-24
• [Presentation] Distinct mechanisms for differentiation of myeloid cells in the anterior and posterior ventral blood islands in Xenopus embryo (2010)
  • Author(s): Maeno, M., et al.
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: Fairmont Chateau Lake Louise, Canada
  • Year and Date: 2010-09-13
• [Presentation] Disfinct mechanisms for differentiation of myeloid cells in the anterior and posterior ventral blood islands in Xeuopus embryo. (2010)
  • Author(s): Maeno M, Matsuzaki Y, others
  • Organizer: 13th International Xenopus Conference
  • Place of Presentation: Fairmont Chateau LakeLouise, Canada
  • Year and Date: 2010-09-13
• [Presentation] Functional analysis of VEGF in myeloid cell differentiation in the ventral blood islands in Xenopus embryo (2010)
  • Author(s): 大竹亜希子ほか
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-06-21
• [Presentation] A novel role of Neptune in induction of the hatching gland and neural crest in the Xenopus embryo (2010)
  • Author(s): 倉内孝行ほか
  • Organizer: 第43回日本発生生物学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-06-21
• [Presentation] Functional analysis of VEGF in myeloid cell differentiation in the ventral blood islands in Xenop us embryo (2010)
  • Author(s): 大竹亜希子, 栗原沙也香, ほか
  • Organizer: 日本発生生物学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-06-21
• [Presentation] A novel role of Neptune in induction of the hatching gland and neural crest in the Xenopua embryo (2010)
  • Author(s): 倉内孝行, 星野亨, ほか
  • Organizer: 日本発生生物学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-06-21
• [Presentation] Lipopolysaccharide administration in adult African clawed frog as an animal model for leukocytosis (2010)
  • Author(s): Onda, N., et al
  • Organizer: 39th Annual Scientific Meeting of ISEH-Society for Hematology and Stem Cells
  • Place of Presentation: Melbourne, Australia
• [Presentation] The production and destruction of erythroids in the liver of Xenopus laevis (2010)
  • Author(s): Okui, T., et al.
  • Place of Presentation: Melbourne, Australia.
• [Presentation] Lipopolysaccharide administration in adult African clawed frog as an animal model for leukocytosis. (2010)
  • Author(s): Onda N, Onodera H, Kato K, Ishida K, Mano Y, Nagasawa K, Akatsuka, Maeno M, Kato T.
  • Organizer: 39th Annual Scientific Meeting of ISEH-Society for Hematology and Stem Cells.
  • Place of Presentation: Melbourne, Australia
• [Presentation] The production and destruction of erythroids int the liver of Xenopus laevis. (2010)
  • Author(s): Okui T, Beppu M, Mano Y, Kohama S, Maekawa S, Yamamoto Y, Kato T.
  • Organizer: 39th Annual Scientific Meeting of ISEH-Society for Hematology and Stem Cells.
  • Place of Presentation: Melbourne, Australia
• [Presentation] Hepatic erythropoiesis in adult Xenopus laevis during anemia (2009)
  • Author(s): Okui, T., et al.
  • Organizer: 51th Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleanss, Louisiana, USA.
  • Year and Date: 2009-12-05
• [Presentation] Hepatic erythropoiesis in adult Xenopus laevis during anemia (2009)
  • Author(s): Takehito Okui, Takashi Kato
  • Organizer: 51th Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleanss, Louisiana, USA
  • Year and Date: 2009-12-05
• [Presentation] アスパラギン結合型糖鎖を欠失するアフリカツメガエルエリスロポエチンの熱安定性の解析 (2009)
  • Author(s): 目黒瑞枝ほか
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-14
• [Presentation] 末梢好中球増多にともなうアフリカツメガエル好中球産生調節因子の発現の変化 (2009)
  • Author(s): 加藤友啓ほか
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-19
• [Presentation] アフリカツメガエルにおける赤血球系細胞の免疫染色と細胞蛋白質変動の検出 (2009)
  • Author(s): 別府実穂ほか
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-19
• [Presentation] リポ多糖投与アフリカツメガエルにおける好中球数の増多と組織貯蔵 (2009)
  • Author(s): 恩田信洋ほか
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-19
• [Presentation] 末梢好中球増多にともなうアフリカツメガエル好中球産生調節因子の発現の変化 (2009)
  • Author(s): 加藤友啓, 小野寺秀和, 恩田信洋, 赤塚龍平, 松田悠, 加藤尚志
  • Organizer: 第80回 日本動物学会大会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-19
• [Presentation] アフリカツメガエルにおける赤血球系細胞の免疫染色と細胞蛋白質変動の検出 (2009)
  • Author(s): 別府実穂, 奥井武仁, 小坂-野川菜美, 會沢洋一, 加藤尚志
  • Organizer: 第80回 日本動物学会大会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-19
• [Presentation] リポ多糖投与アフリカツメガエルにおける好中球数の増多と組織貯蔵 (2009)
  • Author(s): 恩田信洋, 小野寺秀和, 加藤友啓, 真野陽介, 松田悠, 赤塚龍平, 加藤尚志
  • Organizer: 第80回 日本動物学会大会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-19
• [Presentation] アフリカツメガエル胚においてnkx2. 5は白血球の分化に関与している (2009)
  • Author(s): 松崎陽子ほか
  • Organizer: 第80回日本動物学会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-17
• [Presentation] アフリカツメガエル胚においてnkx2.5は白血球の分化に関与している (2009)
  • Author(s): 松崎陽子, ほか
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-17
• [Presentation] 両生類造血に学ぶ~アフリカツメガエル血球産生のダイナミクス~ (2009)
  • Author(s): 小坂(野川)菜美ほか
  • Organizer: 第日本比較免疫学会第21回学術集会シンポジウム「リンパ球の起源と分化」
  • Place of Presentation: 日本大学(神奈川)
  • Year and Date: 2009-08-05
• [Presentation] 両生類造血に学ぶ～アフリカツメガエル血球産生のダイナミクス～ (2009)
  • Author(s): 小坂(野川)菜美, 前川峻, 永澤和道, 家村仁美, 奥井武仁, 加藤尚志
  • Organizer: 第日本比較免疫学会第 21回学術集会 シンポジウム「リンパ球の起源と分化」
  • Place of Presentation: 日本大学(神奈川)
  • Year and Date: 2009-08-05
• [Presentation] Role of canonical Wnt signal in myeloid cell differentiation in the ventral blood islands of Xenopus embryo (2009)
  • Author(s): 小宮山恭吾ほか
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: 新潟朱鷺メッセ
  • Year and Date: 2009-05-29
• [Presentation] Role of canonical Wnt signal in myeloid cell differentiation in the ventral blood islands of Xenopus embryo (2009)
  • Author(s): 小宮山恭吾, ほか
  • Organizer: 日本発生生物学会
  • Place of Presentation: 新潟朱鷺メッセ
  • Year and Date: 2009-05-29
• [Presentation] AP-2rep is involved in morphogenesis of the axial mesoderm in Xenopus embryo (2009)
  • Author(s): 斎藤吉成ほか
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: 新潟朱鷺メッセ
  • Year and Date: 2009-05-28
• [Presentation] AP-2rep is involved in morphogenesis of the axial mesoderm in Xenopus embryo (2009)
  • Author(s): 斎藤吉成, ほか
  • Organizer: 日本発生生物学会
  • Place of Presentation: 新潟朱鷺メッセ
  • Year and Date: 2009-05-28
• [Remarks]
  • URL: http://www.f.waseda.jp/tkato/index.html
• [Remarks]
  • URL: http://www.f.waseda.jp/tkato/index.html