| Project/Area Number |
21580008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TERACHI Toru 京都産業大学, 総合生命科学部, 教授 (90202192)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 植物育種 / 遺伝 / 稔性回復遺伝子 / 細胞質雄性不稔 / オルガネラゲノム / ダイコン / ミトコンドリア / ハマダイコン / orf138 / 雄性不稔 / クローニング |
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| Research Abstract |
We conducted the experiments to isolate Rf genes for the Ogura male-sterility and identify their characteristics. We found in Japanese wild radishes new Rft gene that is involved in the processing of mRNA of orf138, and exploited DNA markers tightly linked to the Rft. Furthermore, it was demonstrated that unique Rf genes are distributed in wild species, Raphanus raphanistrum, Japanese wild radish, and Chinese cultivated radishes, respectively. The DNA sequences of them were determined. Based on the results, the genetic and evolutionary processes of establishment of each Rf gene were estimated. This research project shows, as a whole, the diverse differentiations of Rf genes in wild and cultivated radishes.
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