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Isolation of the gene controlling inflorescence types in Hydrangea macrophylla by utilizing the phenomenon of constitutive activity of retrotransposon

Research Project

Project/Area Number 21580039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionThe University of Shiga Prefecture

Principal Investigator

UEMACHI Tatsuya  滋賀県立大学, 環境科学部, 助教 (40243076)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsレトロトランスポゾン / アジサイ / 芽条変異 / トランスポゾンディスプレイ / 花房型 / 二本鎖RNA / RNA干渉
Research Abstract

RT-PCR analysis indicated that LTR retrotransposons were constitutively transcribed in nine Hydrangea species and one Cardiandra species. The dsRNAs isolated from flower buds of' BM-1' which were a mutant of 'Blue Sky' were homologous to a partial sequence of retrotransposon. Transposon display analysis indicated that transposon insertion frequently occurred in 'Blue Sky'.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (7 results)

All 2010 2009

All Presentation (7 results)

  • [Presentation] アジサイの花房型変異株からのレトロトランスポゾン様2本鎖RNA配列の単離2010

    • Author(s)
      北村太郎, 上町達也
    • Organizer
      園芸学会
    • Place of Presentation
      大分大学(大分県)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] レトロトランスポゾンの転写が生じているアジサイ属植物の特定2010

    • Author(s)
      上町達也, 菱田美紀, 右川紗矢佳, 奥村麻未, 林憲司
    • Organizer
      園芸学会
    • Place of Presentation
      日本大学(神奈川県)
    • Year and Date
      2010-03-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] レトロトランスポゾンの転写が生じているアジサイ属植物の特定2010

    • Author(s)
      上町達也, 菱田美紀, 右川紗矢佳, 奥村麻未, 林憲司
    • Organizer
      園芸学会平成22年度春季大会
    • Place of Presentation
      藤沢市
    • Related Report
      2011 Final Research Report
  • [Presentation] アジサイの花房型変異株からのレトロトランスポゾン様2本鎖RNA配列の単離2010

    • Author(s)
      北村太郎, 上町達也
    • Organizer
      園芸学会平成22年度秋季大会
    • Place of Presentation
      大分市
    • Related Report
      2011 Final Research Report
  • [Presentation] Expression analysis of retrotransposon in Hydrangea macrophylla' Blue Sky' and its sport2010

    • Author(s)
      Okumura, Y. Kitamura, K. Hayashi
    • Organizer
      28th International Horticultural Congress
    • Place of Presentation
      Lisbon, Portugal
    • Related Report
      2011 Final Research Report
  • [Presentation] アジサイ及びヤマアジサイ品種及び野生種におけるレトロトランスポゾンの転写2009

    • Author(s)
      上町達也, 右川紗矢佳, 菱田美紀, 奥村麻未, 林憲司
    • Organizer
      園芸学会
    • Place of Presentation
      秋田大学(秋田県)
    • Year and Date
      2009-09-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] アジサイ及びヤマアジサイ品種及び野生種におけるレトロトランスポゾンの転写2009

    • Author(s)
      上町達也, 右川紗矢佳, 菱田美紀, 奥村麻未, 林憲司
    • Organizer
      園芸学会平成19年度秋季大会
    • Place of Presentation
      秋田市
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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