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Studies of microbial community structure and function for pathogen bank in field margins

Research Project

Project/Area Number 21580052
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionChiba University

Principal Investigator

SHISHIDO Masahiro  千葉大学, 大学院・園芸学研究科, 准教授 (80302537)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords感染・増殖 / 病原菌生態 / 微生物群集 / フィールド・マージン / 病原菌バンク / リアルタイムPCR / 土壌伝染性病害
Research Abstract

Ecology of black root rot of cucurbittaceae and white root rot of Japanese pear were studied to elucidate functions for pathogen bank in field margins. After developing real-time PCR protocols to quantify these plant pathogens, relationships between pathogen quantities and microbial community structures of both in-fields and field margins. The research results suggested that pathogens were least proliferate in field margins, mostly remaining inside the fields, and affected only particular groups of microorganisms.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (16 results)

All 2012 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (8 results) Book (1 results)

  • [Journal Article] Development of real-time PCR assayusing Taq Man probe for specific and quantitative detection of Rosellinia necatrix in plant and soil2012

    • Author(s)
      Shishido, M., Kubota, I., Nakamura, H
    • Journal Title

      Journal of General Plant Pathology

      Volume: 78(2) Issue: 2 Pages: 115-120

    • DOI

      10.1007/s10327-012-0366-x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of jasmonates on ethylene biosynthesis and aroma volatile emission in Japanese apricot infected by a pathogen(Colletotrichum gloeosporioides)2011

    • Author(s)
      Nimitkeatkai, H., Shishido, M., Okawa, K., Ohara, H., Ban, Y., Kita, M., Moriguchi, T., Ikeura, H., Hayata, Y., Kondo, S
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 59(12) Issue: 12 Pages: 6423-6429

    • DOI

      10.1021/jf2010996

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plant disease management in protected horticulture(review)2011

    • Author(s)
      Shishido M
    • Journal Title

      Hort Research

      Volume: 65 Pages: 7-18

    • URL

      http://www.h.chiba-u.ac.jp/chiiki/hortresearch.html

    • Related Report
      2011 Final Research Report
  • [Journal Article] Plant disease management in protected horticulture2011

    • Author(s)
      Shishido, M.
    • Journal Title

      HortResearch

      Volume: 65(in press)

    • NAID

      40018926275

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PCR-based assays to detect and quantify Phomopsis sclerotioides in plants and soil2010

    • Author(s)
      Shishido, M., Sato, K., Yoshida, N., Tsukui, R., Usami, T
    • Journal Title

      Journal of General Plant Pathology

      Volume: 76(1) Issue: 1 Pages: 21-30

    • DOI

      10.1007/s10327-009-0209-6

    • Related Report
      2011 Final Research Report
  • [Journal Article] PCR-based assays to detect and quantify Phomopsis sclerotioides in plants and soil.2010

    • Author(s)
      Shishido, M., Sato, K., Yoshida, N., Tsukui, R., Usami, T.
    • Journal Title

      Journal of General Plant Pathology 76

      Pages: 21-30

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物葉圏における細菌群集の解析2009

    • Author(s)
      須田亙・宍戸雅宏
    • Journal Title

      土と微生物 63

      Pages: 93-99

    • NAID

      110009468643

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Development of real-time PCR assay using Taq Man probefor detection and quantification of Monosporascus cannonballus in plant andsoil2012

    • Author(s)
      Shishido, M., Yoshida, N., Tsukui1, R., Yokoyama, T., Usami, T
    • Organizer
      The 2nd Korea-Japan Joint Symposium,The Phytopathological Society of Japan(PSJ) and The Korean Society of Plant Pathology (KSPP)
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of real-time PCR assay using TaqMan probe for detection and quantification of Monosporascus cannonballus in plant and soil2012

    • Author(s)
      Shishido, M., Yoshida, N., Tsukui1, R., Yokoyama, T., Usami, T.
    • Organizer
      The 2nd Korea-Japan Joint Symposium, The Phytopathological Society of Japan (PSJ) and The Korean Society of Plant Pathology (KSPP)
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2011 Annual Research Report
  • [Presentation] TaqMan probeを用いたリアルタイムPCRによる白紋羽病菌の定量と検出2011

    • Author(s)
      宍戸雅宏, 久保田斎, 中村仁
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      大崎市鳴子公民館
    • Related Report
      2011 Final Research Report
  • [Presentation] TaqManプローブを用いたリアルタイムPCRによるPhomopsis sclerotioides検出および定量法の改良2011

    • Author(s)
      宍戸雅宏, 久保田斎, 大橋竜也
    • Organizer
      平成23年度日本植物病理学会大会
    • Place of Presentation
      東京農工大学
    • Related Report
      2011 Final Research Report
  • [Presentation] TaqMan probeを用いたリアルタイムPCRによる白紋羽病菌の定量と検出2011

    • Author(s)
      宍戸雅宏・久保田斎・中村仁
    • Organizer
      日本土壌微生物学会大会
    • Place of Presentation
      大崎市鳴子公民館
    • Related Report
      2011 Annual Research Report
  • [Presentation] ホモプシス根腐病菌はウリ科作物に宿主特異性を示さない2010

    • Author(s)
      宍戸雅宏
    • Organizer
      平成22年度日本植物病理学会大会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2011 Final Research Report
  • [Presentation] ホモプシス根腐病菌はウリ科作物に宿主特異性を示さない2010

    • Author(s)
      宍戸雅宏
    • Organizer
      日本植物病理学会大会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2010 Annual Research Report
  • [Presentation] エンバク野生種すき込みによる後作ナスの半身萎凋病抑制効果と根圏微生物群集構造の変化2009

    • Author(s)
      宍戸雅宏・岩本(小島)育子・須田亙・牛尾進吾
    • Organizer
      日本土壌微生物学会大会
    • Place of Presentation
      九州大学
    • Year and Date
      2009-06-12
    • Related Report
      2009 Annual Research Report
  • [Book] スイカホモプシス根腐病,メロンホモプシス根腐病(植物病大事典)2012

    • Author(s)
      宍戸雅宏, 岸國平・小林享夫・堀江博道編
    • Publisher
      全国農村教育協会(インターネット版)
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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