Post genome and functional analysis of beneficial Rhizobium genes for maintaining symbiotic nitrogen fixation of nodules

• Project/Area Number: 21580079
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Kagawa University
• Principal Investigator: TAJIMA Shigeyuki 香川大学, 農学部, 教授 (50116894)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 共生窒素固定 / プロテオミクス / 共生特異的遺伝子 / ダイズ根粒 / 金属シャペロン / バクテロイド / 植物・根粒菌相互作用 / シトクロームcオキシターゼ / シトクロームCオキシダーゼ / 根粒菌 / ダイズ / プロテオーム解析 / ペプチドマスフィンガープリント(PMF)法 / シトクロームcオキシダーゼ / FixNOPQ

Research Abstract

Using 2D-electrophoresis proteomics, 278 proteins in B. japonicum and 359 in Mesorhizobium loti were identified. In Glycine max mitochondria 272 proteins and in Lotus japonicas mitochondria 198 proteins were identified. The database of these protein expressions was constructed concerning to nodule development. The functional analysis of these genes revealed proteins which have key role for maintaining nitrogen fixation.

Research Products

• [Journal Article] Rare sugar D-allose suppresses gibberellin signaling through hexokinase-dependent pathway in Oryza sativa L. (2011)
  • Author(s): Takeshi Fukumoto, Akihito Kano, Kouhei Ohtani, Yumiko Yamasaki-Kokudo, Bong-Gyu Kim, Kouji Hosotani, Miu Saito, Chikage Shirakawa, Shigeyuki Tajima, Ken Izumori^1, Toshiaki Ohara, Yoshio Shigematsu, Keiji Tanaka, Yutaka Ishida, Yoko Nishizawa, Yasuomi Tada, Kazuya Ichimura, Kenji Gomi and Kazuya Akimitsu
  • Journal Title: Planta Volume: 234 Pages: 1083-1095
  • Peer Reviewed
• [Journal Article] Rare sugar D-allose suppresses gibberellin signaling through hexokinase-dependent pathway in Oryza sativa L (2011)
  • Author(s): Fukumoto, T., Tajima, S., 他
  • Journal Title: Planta Volume: 234 Pages: 1083-1095
  • Peer Reviewed
• [Journal Article] Temperature-Dependent Expression of Type III Secretion System Genes and Its Regulation in Bradyrhizobium japonicum (2010)
  • Author(s): Min Wei, Keisuke Takeshima, Tadashi Yokoyama, Kiwamu Minamisawa, Hisayuki, Mitsui, Manabu Itakura, Takakazu Kaneko, Satoshi Tabata, Kazuhiko Saeki, Hirofumi Omori, Shigeyuki Tajima, Toshiki Uchiumi, Mikiko Abe, Satoshi Ishii, and Takuji Ohwada
  • Journal Title: MPMI Volume: 23 Pages: 628-637
  • Peer Reviewed
• [Journal Article] Expression and characterization of PKL01, an Ndr kinase homolog in Lotus japonicas (2010)
  • Author(s): Isamu Kameshita, Sachiko Shimomura, Kazushi Nishio, Noriyuki Sueyoshi, Tetsuyuki Nishida, Mika Nomura, and Shigeyuki Tajima
  • Journal Title: Journal of Biochemistry Volume: 147 Pages: 799-807
  • Peer Reviewed
• [Journal Article] Differential protein profiles of Bradyrhizobium japonicum USDA110 bacteroid in soybean nodule development (2010)
  • Author(s): Mika Nomura, Hattaya Arunothayanan, Tan Van Dao, Hoa Thi-Phuong Le, Takakazu Kaneko, Syusei Sato, Satoshi Tabata and Shigeyuki Tajima
  • Journal Title: Soil Sci. Plant Nutrition Volume: 56 Pages: 579-590
  • Peer Reviewed
• [Journal Article] NAD-malic enzyme, not NADP-malic enzyme, affects nitrogenase activity of Mesorhizobium loti bacteroids in root nodules (2010)
  • Author(s): Nanthipak Thapanapongworakul, Mika Nomura, Yoshikazu Shimoda, Shusei Sato, Satoshi Tabata and Shigeyuki Tajima
  • Journal Title: Plant Biotech Volume: 27 Pages: 311-316
  • Peer Reviewed
• [Journal Article] Copper metallochaperones are required for the assembly of bacteroid cytochrome c oxidase which is functioning for nitrogen fixation in soybean nodules (2010)
  • Author(s): Hatthaya Arunothayanan, Mika Nomura, Rie Hamaguchi, Manabu Itakura, Kiwamu Minamisawa and Shigeyuki Tajima
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 1-5
  • NAID: 10027351117
  • Peer Reviewed
• [Journal Article] 3-phosphoglycerate dehydrogenase in Mesorhizobium loti is essential for maintaining symbitotic nitrogen fixation of Lotus japonicus root nodules (2010)
  • Author(s): Nanthipak Thapanaponggworakul, Mika, Nomura, Van Dao Tan, Yoshikazu Shimoda, Syusei Sato, Satoshi Tabata and Shigeyuki Tajima
  • Journal Title: Plant and Soil Volume: 336 Pages: 233-240
  • Peer Reviewed
• [Journal Article] Copper metallochaperones are required for the assembly of bacteroid cytochrome c oxidase which is functioning for nitrogen fixation in soybean nodules (2010)
  • Author(s): Arunothayanan, H., et al.
  • Journal Title: Plant and Cell Physiol. Volume: 51 Pages: 1242-1246
  • Peer Reviewed
• [Journal Article] Differential protein profiles of Bradyrhizobium japonicum USDA110 bacteroid during soybean nodule development (2010)
  • Author(s): Nomura, M., et al.
  • Journal Title: Soil Science and Plant Nutr. Volume: 56 Pages: 579-590
  • Peer Reviewed
• [Journal Article] 3-Phosphoglycerate dehydrogenase in Mesorhizobium loti is essential for maintaining symbiotic nitrogen fixation of Lotus japonicus root nodules. (2010)
  • Author(s): Thapanapongworakul, N., et al.
  • Journal Title: Plant and Soil Volume: 336 Pages: 233-240
  • Peer Reviewed
• [Journal Article] NAD^+-malic enzyme affects nitrogenase activity of Mesorhizobium loti bacteroids in Lotus japonicus nodules. (2010)
  • Author(s): Thapanapongworakul, N., et al.
  • Journal Title: Plant Biotech. Volume: 27 Pages: 311-316
  • Peer Reviewed
• [Journal Article] γ-Aminobutyric acid (GABA) Accumulations in rice during germination. (2010)
  • Author(s): Jannoey, P., et al.
  • Journal Title: Chiang Mai J.Sci. Volume: 37 Pages: 124-133
  • Peer Reviewed
• [Journal Article] Host plant genome overcomes the lack of a bacterial gene for symbiotic nitrogen fixation (2010)
  • Author(s): Tsuneo Hakoyama
  • Journal Title: Nature 462 Pages: 514-517
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 間藤徹
  • Journal Title: 植物栄養学 第2版(文永堂出版) Pages: 36-43
• [Journal Article] Host plant genome overcomes a lack of bacterial gene for symbiotic nitrogen fixation (2009)
  • Author(s): Tsuneo Hakoyama, Kaori Niimi, Hirokazu Watanabe, Ryohei Tabata, Junichi Matsubara, Shusei Sato, Yasukazu Nakamura, Satoshi Tabata, Li Jichun, Tsuyoshi Matsumoto, Kazuyuki Tatsumi, Mika Nomura, Shigeyuki Tajima, Masumi Ishizaka, Koji Yano, Haruko Imaizumi-Anraku, Masayoshi Kawaguchi, Hiroshi Kouchi& Norio Suganuma
  • Journal Title: Nature Volume: 462 Pages: 514-517
  • NAID: 120002146280
  • Peer Reviewed
• [Presentation] Nanthipak Thapanapongworakul, Ayao Enoki and Hiroyuki Matsuura、FUNCTIONAL ANALYSIS OF AMINO ACID METABOLISM MUTANTS OF MESORHIZOBIUM LOTI (2011)
  • Author(s): Shigeyuki Tajima, Mika Nomura
  • Organizer: 第17回国際窒素固定会議
  • Place of Presentation: オーストラリア
• [Presentation] ミヤコグサ根粒菌の共生窒素固定に関わるアミノ酸代謝の解析 (2011)
  • Author(s): 松浦寛幸, 榎彩緒, Nantipak Thapanapongworakul, 佐藤修正, 下田宜司, 野村美加, 田島茂行
  • Organizer: 植物微生物研究会
  • Place of Presentation: 岡山大学
• [Presentation] ミヤコグサ根粒菌変異体を用いた共生窒素固定の解析 (2011)
  • Author(s): 野村美加, 松浦寛幸, 榎彩緒, 田島茂行
  • Place of Presentation: 国際会議場
• [Presentation] FUNCTIONAL ANALYSIS OF AMINO ACID METABOLISM MUTANTS OF MESORHIZOBIUM LOTI (2011)
  • Author(s): Shigeyuki Tajima, Mika Nomura, Nanthipak Thapanapongworakul, Ayao Enoki, Hiroyuki Matsuura
  • Organizer: 17回国際窒素固定会議
  • Place of Presentation: Australia
• [Presentation] ミヤコグサ根粒菌の共生窒素固定に関わるアミノ酸代謝の解析 (2011)
  • Author(s): 松浦寛幸, 榎彩緒, Nantipak Thapanapongworakul, 佐藤修正, 下田宜司, 野村美加, 田島茂行
  • Organizer: 植物微生物研究会
  • Place of Presentation: 岡山大学(岡山)
• [Presentation] ミヤコグサ根粒菌変異体を用いた共生窒素固定の解析 (2011)
  • Author(s): 野村美加, 松浦寛幸, 榎彩緒, 田島茂行
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: つくば国際会議場(つくば)
• [Presentation] Screening of Mesorhizobium loti mutants for symbiotic N2 fixation in Lotus japonicus (2010)
  • Author(s): Ayao Enoki, et al.
  • Organizer: 第一回植物微生物共生と窒素固定に関するアジア国際会議
  • Place of Presentation: 青島パームビーチホテル(宮崎県)
• [Presentation] Functional analysis of Gen06 genes in Lotus japonicus nodules (2010)
  • Author(s): Yousuke Hase, et al.
  • Organizer: 第一回植物微生物共生と窒素固定に関するアジア国際会議
  • Place of Presentation: 青島パームビーチホテル(宮崎県)
• [Presentation] 香川県春日川河口域のアオサ種の同定及びその生理学的応答 (2010)
  • Author(s): 竹藤豊, 他
  • Organizer: 日本陸水学会
  • Place of Presentation: 弘前大学
• [Presentation] 希少糖D-PsicoseにおよびD-Alloseのイネに対する生育阻害効果の解析 (2010)
  • Author(s): 細川欣秀, 他
  • Organizer: 土壌肥料学会
  • Place of Presentation: 北海道大学
• [Presentation] 共生窒素固定に関わるミヤコグサ根粒菌遺伝子の検索 (2009)
  • Author(s): 榎綾緒
  • Organizer: 日本土壌肥料学会関西支部
  • Place of Presentation: 高知 高知城ホール
  • Year and Date: 2009-12-11