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Study on determination of pyrroloquinoline quinine derivatives in various foods

Research Project

Project/Area Number 21580154
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionShowa University

Principal Investigator

KUMAZAWA Takeshi  昭和大学, 医学部, 教授 (00186470)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords食品化学 / 栄養生化学 / ピロロキノリンキノン / HPLC法 / MS/MS法 / MS / MS法
Research Abstract

In the present study, we examined the determination of pyrroloquinoline quinone(PQQ) derivatives in various foods that are commonly consumed daily. The samples were analyzed using efficient extraction, accurate identification and quantification followed by high performance liquid chromatography-tandem mass spectrometry. The PQQ amino acid derivatives were identified with low concentration in various foods. The imidazopyrroloquinoline concentrations ranged from several ng/g-several hundred ng/g(or ml); other PQQ derivatives were in the range of several ng/g or ml.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (17 results)

All 2011 2010 2009 Other

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (3 results)

  • [Journal Article] Determination of dextromethorphan in human plasma using pipette tip solid-phase extraction and gas chromatography-mass spectrometry2011

    • Author(s)
      Hasegawa C., Kumazawa T., Uchigasaki S., Lee X.-P., Sato K., Terada M., Kurosaki K
    • Journal Title

      Anal. Bioanal. Chem

      Volume: 401 Pages: 2215-2223

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fragmentation pathways of trifluoroacetyl derivatives of methamphetamine, amphetamine, and methylenedioxyphenylalkylamine designer drugs by gas chromatography/mass spectrometry2011

    • Author(s)
      Kumazawa T., Hara K., Hasegawa C., Uchigasaki S., Lee X.-P., Seno H., Suzuki O., Sato K
    • Journal Title

      Int. J. Spectrosc

      Volume: 2011 Pages: 1-12

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative determination of phenothiazine derivatives in human plasma using monolithic silica solid-phase extraction tips and gas chromatography-mass spectrometry2011

    • Author(s)
      Kumazawa T., Hasegawa C., Uchigasaki S., Lee X.-P., Suzuki O., Sato K
    • Journal Title

      J. Chromatogr

      Volume: 1218 Pages: 2521-2527

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative determination of phenothiazine derivatives in human plasma using monolithic silica solid-phase extraction tips and gas chromatography-mass spectrometry2011

    • Author(s)
      Takeshi Kumazawa
    • Journal Title

      Journal of Chromatography A

      Volume: VOL.1218 Pages: 2521-2527

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fragmentation pathways of trifluoroacetyl derivatives of methamphetamine, amphetamine, and methylenedioxyphenylalkylamine designer drugs by gas chromatography/mass spectrometry2011

    • Author(s)
      Takeshi Kumazawa
    • Journal Title

      International Journal of Spectroscopy

      Volume: VOL.2011 Issue: 1 Pages: 1-12

    • DOI

      10.1155/2011/318148

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of dextromethorphan in human plasma using pipette tip solid-phase extraction and gas chromatography-mass spectrometry2011

    • Author(s)
      Chika Hasegawa
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: VOL.401 Pages: 2215-2223

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] シリカモノリス固相抽出技術と薬毒物分析への応用2010

    • Author(s)
      熊澤武志, 長谷川智華, 李暁鵬, 佐藤啓造, 鈴木修
    • Journal Title

      JSBMS Lett

      Volume: 35 Pages: 32-37

    • Related Report
      2011 Final Research Report
  • [Journal Article] New and unique methods of solid-phase extraction for use before instrumental analysis of xenobiotics in human specimens2010

    • Author(s)
      Kumazawa T., Hasegawa C., Lee X.-P., Sato K
    • Journal Title

      Forensic Toxicol

      Volume: 28 Pages: 61-68

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of strain differences and vehicles on results of local lymph node assays2010

    • Author(s)
      Anzai T., Ullmann L. G., Hayashi D., Satoh T., Kumazawa T., Sato K
    • Journal Title

      Exp. Anim

      Volume: 59 Pages: 245-249

    • NAID

      130000254353

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Determination of non-steroidal anti-inflammatory drugs in human plasma by LC-MS-MS with a hydrophilic polymer column2010

    • Author(s)
      Lee X.-P., Kumazawa T., Hasegawa C., Arinobu T., Kato A., Seno H., Sato K
    • Journal Title

      Forensic Toxicol

      Volume: 28 Pages: 96-104

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term storage of blood samples as freezing hemolysates with Good's buffer for methemoglobin determination2010

    • Author(s)
      Arima Y., Sato K., Fujishiro M., Nittono S., Uji A., Marumo A., Lee X.-P., Kumazawa T., Katsumata Y
    • Journal Title

      Med. Biol

      Volume: 154 Pages: 114-120

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activation of NFAT signal by p53-K120R mutant2009

    • Author(s)
      Shinmen N., Koshida T., Kumazawa T., Sato K., Shimada H., Matsutani T., Iwadate Y., Takiguchi M., Hiwasa T
    • Journal Title

      FEBS Lett

      Volume: 583 Pages: 1916-1922

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Automated on-line in-tube solid-phase microextraction coupled with HPLC/MS/MS for the determination of butyrophenone derivatives in human plasma2009

    • Author(s)
      Kumazawa T., Saeki K., Yanagisawa I., Uchigasaki S., Hasegawa C., Seno H., Suzuki O., Sato O
    • Journal Title

      Anal. Bioanal. Chem

      Volume: 394 Pages: 1161-1170

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecularly imprinted solid-phase extraction for selective determination of methamphetamine, amphetamine, and methylenedioxyphenylalkylamine designer drugs in human whole blood by gas chromatography-mass spectrometry

    • Author(s)
      Kumazawa T., Hasegawa C., Hara K., Uchigasaki S., Lee X.-P., Seno H., Suzuki O., Sato K
    • Journal Title

      J. Sep. Sci

      Volume: (印刷中)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] シリカモノリスSPEとGC/MSを利用した血漿中フェノチアジン系向精神薬の分析2010

    • Author(s)
      熊澤武志, 長谷川智華, 李暁鵬, 有信哲哉, 妹尾洋, 鈴木修, 佐藤啓造
    • Organizer
      第35回日本医用マススペクトル学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] オンライン自動カラムスイッチングUFLC-MS/MS及びMS3によるヒト血漿中ベンゾジアゼピン系薬物の迅速高感度分析法2010

    • Author(s)
      李暁鵬, 熊澤武志, 川瀬靖聡, 長谷川智華, 林大吾, 有信哲哉, 佐藤啓造
    • Organizer
      第35回日本医用マススペクトル学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] モノリスカラムを用いたバックフラッシュカラムスイッチングLC/MSによる血中テオフィリンの超迅速分析2009

    • Author(s)
      有信哲哉, 服部秀樹, 熊澤武志, 李暁鵬, 水谷洋子, 大森貴之, 西本寛, 小島貞男, 石井晃, 妹尾洋
    • Organizer
      第34回日本医用マススペクトル学会年会
    • Place of Presentation
      大阪
    • Year and Date
      2009-09-10
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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