Quantification of insulin-like growth factor binding proteins in salmon

• Project/Area Number: 21580214
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: SHIMIZU Munetaka 北海道大学, 大学院・水産科学研究院, 講師 (90431337)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 成長 / ホルモン / サケ科魚類 / 免疫測定系 / 発現解析 / 組換え蛋白 / 発現量測定

Research Abstract

The present study established time-resolved fluoroimmunoassay and real-time quantitative PCR for salmon insulin-like growth factor (IGF)-I and an IGF-binding protein (IGFBP-1b). Feeding and fasting experiment using individually-tagged masu salmon revealed that IGF-I and IGFBP-1b generally show positive and negative correlations with growth rate, respectively. The present study suggests that these components are useful as growth indices in salmon.

Research Products

• [Journal Article] Normalizing for biology : accounting for technical and biological variation in levels of reference gene and insulin-like growth factor 1 (igf1) transcripts in fish livers (2012)
  • Author(s): Metzger, D. C., Luckenbach, J. A., Shimizu, M., and Beckman, B. R.
  • Journal Title: Comparative Biochemistry and Physiology Volume: (in press) Issue: 1 Pages: 7-14
  • DOI: 10.1016/j.cbpa.2012.04.014
  • Peer Reviewed
• [Journal Article] Effects of short-term starvation on ghrelin, GH-IGF system, and IGF-binding proteins in Atlantic salmon (2011)
  • Author(s): Hevroy, E. M., Azpeleta, C., Shimizu, M., Lanzen, A., Kaiya, H., Espe, M, and Olsvik, P. A.
  • Journal Title: Fish Physiology and Biochemistry Volume: 37 Issue: 1 Pages: 217-232
  • DOI: 10.1007/s10695-010-9434-3
  • Peer Reviewed
• [Journal Article] Circulating salmon 41-kDa insulin-like growth factor binding protein (IGFBP) is not IGFBP-3 but an IGFBP-2 subtype (2011)
  • Author(s): Shimizu, M., Suzuki, S., Horikoshi, M., Hara, A., and Dickhoff, W. W.
  • Journal Title: General and Comparative Endocrinology Volume: 171 Issue: 3 Pages: 326-331
  • DOI: 10.1016/j.ygcen.2011.02.013
  • NAID: 120002973854
  • Peer Reviewed
• [Journal Article] Circulating salmon 28-and 22-kDa insulin-like growth factor binding proteins (IGFBPs) are co-orthologs of IGFBP-1 (2011)
  • Author(s): Shimizu, M., Kishimoto, K., Yamaguchi, T., Nakano, Y., Hara, A., and Dickhoff, W. W.
  • Journal Title: General and Comparative Endocrinology Volume: 174 Issue: 2 Pages: 97-106
  • DOI: 10.1016/j.ygcen.2011.08.005
  • NAID: 120003413073
  • Peer Reviewed
• [Journal Article] Effect of short-term starvation on ghrelin, GH-IGF system, and IGF-bin ding proteins in Atlantic salmon (2011)
  • Author(s): Hevroy, E.M., Azpeleta, C., Shimizu, M., Lanzen, A., Kaiya, H., Espe, M., Olsvik, P.A.
  • Journal Title: Fish Physiology and Biochemistry Volume: 37 Pages: 217-232
  • Peer Reviewed
• [Journal Article] Postprandial changes in plasma growth hormone, insulin, insulin-like growth factor (IGF)-I and IGF-binding proteins in coho salmon fasted for varying periods (2009)
  • Author(s): Shimizu, M., Cooper, K. A., Dickhoff, W. W., and Beckman, B. R.
  • Journal Title: American Journal of Physiology-Regulatory, Integrative, and Comparative Physiology Volume: 297 Issue: 2 Pages: R352-R361
  • DOI: 10.1152/ajpregu.90939.2008
  • Peer Reviewed
• [Journal Article] Postprandial changes in plasma growth hormone, insulin, insulin-like growth factor(IGF)-I and IGF-binding proteins in coho salmon fasted for varying period (2009)
  • Author(s): Shimizu, M., Cooper, K.A., Dickhoff, W.W., Beckman, B.R.
  • Journal Title: American Journal of Physiology-Regulatory, Integrative, and Comparative Physiology 297
  • Peer Reviewed
• [Presentation] サクラマスの銀化に伴うインスリン様成長因子-Iの変化と鰓Na+, K+-ATPaseとの関係 (2012)
  • Author(s): 下村考弘・中嶋拓郎・堀越萌李・飯嶋亜内・卜部浩一・水野伸也・平松尚志・原彰彦・清水宗敬
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(品川、東京)
  • Year and Date: 2012-03-28
• [Presentation] サクラマスの銀化変態期における鰓Na+/K+-ATPase活性とインスリン様成長因子-Iの関係 (2011)
  • Author(s): 下村考弘・中嶋拓郎・堀越萌李・飯嶋亜内・水野伸也・卜部浩一・平松尚志・原彰彦・清水宗敬
  • Organizer: 第5回サケ学研究会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-12-18
• [Presentation] サクラマスのおけるインスリン様成長因子結合蛋白-1の発現パターンと成長との関係 (2011)
  • Author(s): 川口航平・下村考弘・中野裕介・木村志津雄・原彰彦・清水宗敬
  • Organizer: 第5回サケ学研究会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-12-18
• [Presentation] サクラマスにおけるインスリン様成長因子結合蛋白-1の発現パターンと成長との関係 (2011)
  • Author(s): 下村孝弘・中嶋拓郎・堀越萌李・飯嶋亜内・卜部浩一・水野伸也・平松尚志・原彰彦・清水宗敬
  • Organizer: 第5回サケ学研究会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-12-12
• [Presentation] 成長のメカニズムからサケ科魚類の生活史多型と資源管理を考える (2011)
  • Author(s): 清水宗敬
  • Organizer: 日本生態学会第58回大会
  • Place of Presentation: 北海道札幌市、札幌コンベンションセンター 招待講演
  • Year and Date: 2011-03-10
• [Presentation] 生理学を武器にして動物生態学をおもしろくできないだろうか? (2011)
  • Author(s): 清水宗敬
  • Organizer: 日本生態学会第58回全国大会
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
• [Presentation] サクラマスにおける海水適応能の発達とインスリン様成長因子-Iの発現パターン (2010)
  • Author(s): 下村考弘・堀越萌李・中嶋拓郎・飯嶋亜内・卜部浩一・水野伸也・平松尚志・原彰彦・清水宗敬
  • Organizer: 第4回サケ学研究会
  • Place of Presentation: 北海道大学(函館市)
  • Year and Date: 2010-12-18
• [Presentation] サクラマスにおける海水適応能の発達とインスリン様成長因子-Iの発現パターン (2010)
  • Author(s): 清水宗敬
  • Organizer: 第4回サケ学研究会
  • Place of Presentation: 北海道函館市、北海道大学
  • Year and Date: 2010-12-18
• [Presentation] 1回の給餌に対するサケ科魚類の血中インスリン様成長因子-Iと結合蛋白の反応 (2010)
  • Author(s): 清水宗敬
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都府京都市、京都大学
  • Year and Date: 2010-09-23
• [Presentation] サケ科魚類の41-kDaインスリン様成長因子結合蛋白のcDNAクローニング (2010)
  • Author(s): 清水宗敬
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 神奈川県藤沢市、日本大学生物資源科学部
  • Year and Date: 2010-03-27
• [Presentation] 1回の給餌に対するサケ科魚類の血中インスリン様成長因子-Iと結合蛋白の反応 (2010)
  • Author(s): 清水宗敬・Kathleen A. Cooper・Walton W. Dickhoff・Brian R. Beckman
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都大学(京都市)
• [Presentation] サケ科魚類は成長のための「定期預金」を持っていないか? (2009)
  • Author(s): 清水宗敬・鈴木せいら・堀越萌李・原彰彦・Walton W. Dickhoff
  • Organizer: 第3回サケ学研究会
  • Place of Presentation: 北海道立水産孵化場(恵庭市)
  • Year and Date: 2009-12-05
• [Presentation] Which IGF-binding proteins should we look at during smoltification? (2009)
  • Author(s): Munetaka Shimizu
  • Organizer: 8^<th> International Workshop on Smoltification
  • Place of Presentation: Oregon State University(Corvallis, Oregon, USA)
  • Year and Date: 2009-09-23
• [Presentation] Which IGF-binding proteins should we look at during smoltification? (2009)
  • Author(s): Shimizu, M., Suzuki, S., Horikoshi, M., Hara, A., and Dickhoff, W. W.
  • Organizer: 8th International Workshop on Smoltification
  • Place of Presentation: Oregon State University (Corvallis, Oregon, USA)
• [Remarks]
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