Investigation of Fish hematopoiesis

• Project/Area Number: 21580236
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nihon University
• Principal Investigator: MORITOMO Tadaaki 日本大学, 生物資源科学部, 准教授 (20239677)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 魚病 / コイ / 赤血球造血 / エリスロポイエチン / キットリガンド / 魚類 / 造血 / 造血前駆細胞 / コロニー形成法 / 赤血球前駆細胞 / T細胞クローン / ミエロイド基本モデル

Research Abstract

Investigation of Fish hematopoiesis
Co-cultivation of carp hematopoietic cells with supporting cell layer resulted in selective proliferation of CD4 single positive T cells. Using this culture system, several helper T cell-like clones were established. Carp hematopoietic cells were also cultured in semi-solid media in the presence of recombinant erythropoietin, thrombopoietin and stem cell factor. Cells colonies derived from progenitors of erythrocyte and thrombocytes were formed.

Research Products

• [Journal Article] Hematopoietic cell populations in dolphin bone marrow : Analysis of colony formation and differentiation (2011)
  • Author(s): Segawa, T., Itou, T., Suzuki, M., Moritomo, T., Nakanishi, T., and Sakai, T
  • Journal Title: Results in Immunology Volume: 1 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Antiviral protection mechanisms mediated by ginbuna crucian carp interferon gamma isoforms 1 and 2 through two distinct interferon gamma receptors (2011)
  • Author(s): Yabu, T., H.Toda, Y.Shibasaki, K.Araki, M.Yamashita, H.Anzai, N.Mano, Y.Masuhiro, S.Hanazawa, H.Shiba, T.Moritomo, T.Nakanishi
  • Journal Title: J.Biochem. Volume: 150 Issue: 6 Pages: 635-648
  • DOI: 10.1093/jb/mvr108
  • NAID: 10030589144
  • Peer Reviewed
• [Journal Article] Evaluating antigen-specific cytotoxicity of CD8+ T cells in fish by Granzyme B-like activity (2011)
  • Author(s): Toda H, Yabu T, Shiba H, Moritomo T, Nakanishi T
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 141 Issue: 1-2 Pages: 168-172
  • DOI: 10.1016/j.vetimm.2011.02.020
  • Peer Reviewed
• [Journal Article] Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish (2011)
  • Author(s): Toda,H., Saito,Y., Koike,T., Takizawa,F., Araki,K., Yabu,T., Somamoto,T., Suetake,H., Suzuki,Y., Ototake,M., Moritomo,T., and Nakanishi,T.
  • Journal Title: Dev. Comp. Immunol Volume: 35 Issue: 6 Pages: 650-660
  • DOI: 10.1016/j.dci.2011.01.013
  • Peer Reviewed
• [Journal Article] Perforin-dependent cytotoxic mechanism in killing by CD8 positive T cells in ginbuna crucian carp, Carassius auratus langsdorfii (2011)
  • Author(s): Toda H, Araki K, Moritomo T, Nakanishi T
  • Journal Title: Developmental & Comparative Immunology Volume: 35 Issue: 1 Pages: 88-93
  • DOI: 10.1016/j.dci.2010.08.010
  • Peer Reviewed
• [Journal Article] A molecule in teleost fish, related with human MHC-encoded G6F, hasa cytoplasmic tail with ITAM and marks the surface of thrombocytes and in some fishes also of erythrocytes (2011)
  • Author(s): Ohashi, K., F.Takizawa, N.Tokumaru, C.Nakayasu, H.Toda, U.Fischer, T.Moritomo, K.Hashimoto, T.Nakanishi, J.M.Dijkstra
  • Journal Title: Immunogenetics Volume: 62 Pages: 1-17
  • Peer Reviewed
• [Journal Article] クローンギンブナの再生医学研究への応用-魚類の造血幹/前駆細胞の同定- (2010)
  • Author(s): 森友忠昭, 小林功, 片倉文彦, and山口卓哉
  • Journal Title: 日本血栓止血学会誌 Volume: 21 Pages: 42-44
  • NAID: 10026118559
  • Peer Reviewed
• [Journal Article] Clonal growth of carp(Cyprinus carpio) T cells in vitro (2010)
  • Author(s): Yamaguchi, T., Katakura, F., Shitanda, S., Niida, Y., Toda, H., Ohtani, M., Yabu, T., Suetake, H., Moritomo, T., and Nakanishi, T
  • Journal Title: Developmental & Comparative Immunology Volume: 35 Pages: 193-202
  • Peer Reviewed
• [Journal Article] Bone marrow biopsy from the flipper of a dolphin (2010)
  • Author(s): Itou, T., Koie, H., Segawa, T., Kato, M., Yanagisawa, M., Ueda, K., Kuwano, R., Suzuki, M., Moritomo, T., and Sakai, T
  • Journal Title: The Veterinary Journal Volume: 185 Pages: 216-217
  • Peer Reviewed
• [Journal Article] Demonstration of T cell and macrophage progenitors in carp(Cyprinus carpio) kidney hematopoietic tissues. Development of clonal assay system for carp hematopoietic cells (2010)
  • Author(s): Katakura, F., Yamaguchi, T., Yoshida, M., Moritomo, T., and Nakanishi, T
  • Journal Title: Developmental & Comparative Immunology Volume: 34 Pages: 685-689
  • Peer Reviewed
• [Journal Article] Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoietic stem cells (2010)
  • Author(s): Kobayashi, I., Ono, H., Moritomo, T., Kano, K., Nakanishi, T., and Suda, T
  • Journal Title: Blood Volume: 115
  • Peer Reviewed
• [Journal Article] Demonstration of T cell and macrophage progenitors in carp (Cyprinus carpio) kidney hematopoietic tissues (2010)
  • Author(s): Katakura, F., T.Yamaguchi, M.Yoshida, T.Moritomo, T.Nakanishi
  • Journal Title: Developmental & Comparative Immunology Volume: 34 Pages: 685-689
  • Peer Reviewed
• [Journal Article] Clonal growth of carp (Cyprinus carpio) T cells in vitro (2010)
  • Author(s): Yamaguchi, T., F.Katakura, S.Shitanda, Y.Niida, H.Toda, M.Ohtani, T.Yabu, H.Suetake, T.Moritomo, T.Nakanishi
  • Journal Title: Developmental & Comparative Immunology Volume: 35 Pages: 193-202
  • Peer Reviewed
• [Journal Article] Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoietic stem cells (2010)
  • Author(s): Kobayashi, I., H.Ono, T.Moritomo, K.Kano, T.Nakanishi, T.Suda
  • Journal Title: Blood Volume: 115
  • Peer Reviewed
• [Journal Article] クローンギンブナの再生医学研究への応用-魚類の造血幹/前駆細胞の同定- (2010)
  • Author(s): 森友忠昭, 小林功, 片倉文彦, 山口卓哉
  • Journal Title: 日本血栓止血学会譜 Volume: 21 Pages: 42-44
  • NAID: 10026118559
  • Peer Reviewed
• [Journal Article] Kinetics of CD4^+ and CD8 [alpha]+T-cell subsets in graft-versus-host reaction (GVHR) in ginbuna crucian carp Carassius auratus langsdorfii (2010)
  • Author(s): Shibasaki, Y., H.Toda, I.Kobayashi, T.Moritomo, T.Nakanishi
  • Journal Title: Developmental & Comparative Immunology Volume: 34 Pages: 1075-1081
  • Peer Reviewed
• [Journal Article] Co-culture of carp(Cyprinus carpio) kidney haematopoietic cells with feeder cells resulting in long-term proliferation of T-cell lineages (2009)
  • Author(s): Katakura, F., Takizawa, F., Yoshida, M., Yamaguchi, T., Araki, K., Tomana, M., Nakao, M., Moritomo, T., and Nakanishi, T
  • Journal Title: Veterinary immunology and immunopathology Volume: 131 Pages: 127-136
  • Peer Reviewed
• [Journal Article] 魚類における造血幹細胞の同定 (2009)
  • Author(s): 小林功・森友忠昭
  • Journal Title: 血液・腫瘍科 Volume: 58 Pages: 449-454
  • NAID: 40019064234
  • Peer Reviewed
• [Journal Article] Co-culture of carp(Cyprinus carpio)kidney haematopoietic cells with feeder cells resulting in long-term proliferation of T-cell lineages (2009)
  • Author(s): Katakura, F., Takizawa, F., Yoshida, M., Yamaguchi, T., Araki, K., Tomana, M.Nakao, M., Moritomo, T., Nakanishi, T
  • Journal Title: Veterinary immunology and immunopathology 131 Pages: 127-136
  • Peer Reviewed
• [Journal Article] Alloantigen-specific killing is mediated by CD8-positive T cells in fish (2009)
  • Author(s): Toda, H., Shibasaki, Y., Koike, T., Ohtani, M., Takizawa, F., Ototake, M., Moritomo, T., Nakanishi, T.
  • Journal Title: Developmental & Comparative Immunology 33 Pages: 646-652
  • Peer Reviewed
• [Journal Article] 魚類における造血幹細胞の同定 (2009)
  • Author(s): 小林功・森友忠昭
  • Journal Title: 血液・腫瘍科 58 Pages: 450-455
  • NAID: 40019064234
  • Peer Reviewed
• [Presentation] コイ栓球造血におけるTPOとkitlaの作用 (2012)
  • Author(s): 杉江勇二・片倉文彦・藪健史・加藤尚志・森友忠昭・中西照幸
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-29
• [Presentation] コイTh2様細胞のクローン性増殖 (2012)
  • Author(s): 山口卓哉・片倉文彦・Johannes Martinus Dijkstra・森友忠昭・中西照幸
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-29
• [Presentation] コイIL-4/13BのB細胞培養に対する効果 (2012)
  • Author(s): 宮田俊輔・山口卓哉・片倉文彦・柴崎康宏・藪健史・森友忠昭・中西照幸
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-29
• [Presentation] Clonal growth of Carp(Cyprinus carpio) helper T cells-Identification of Th2-like cells (2012)
  • Author(s): Tadaaki Moritomo
  • Organizer: 2012 Taiwan. Japan Symposium on Emerging Trends in Aquatic Animal Immunity and Aquaculture Biotechnology
  • Place of Presentation: National Cheng Kung University
  • Year and Date: 2012-02-22
• [Presentation] コイTPOの遺伝子クローニングおよび性状解析 (2011)
  • Author(s): 林潔高・片倉文彦・藪健史・森友忠昭・中西照幸
  • Organizer: 第23回日本比較免疫学会
  • Place of Presentation: 海洋開発研究機構
  • Year and Date: 2011-08-21
• [Presentation] 魚類の造血機構解明を目的としたコイkit ligand Aの機能解析 (2011)
  • Author(s): 尻無浜由希・片倉文彦・藪健史・森友忠昭・中西照幸
  • Organizer: 第23回日本比較免疫学会
  • Place of Presentation: 海洋開発研究機構
  • Year and Date: 2011-08-21
• [Presentation] ギンブナCD3ε分子の特性解明 (2011)
  • Author(s): 村田憲史、大橋健、柴崎康宏, 藪健史, 森友忠昭、中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 海洋研究開発機構
  • Year and Date: 2011-08-21
• [Presentation] 魚類の造血機構解明を目的としたコイkit ligand Aの機能解析 (2011)
  • Author(s): 尻無浜由希、片倉文彦、藪健史、森友忠昭、中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 海洋研究開発機構
  • Year and Date: 2011-08-21
• [Presentation] ギンブナの移植片対宿主反応におけるCD8陽性T細胞の動態 (2011)
  • Author(s): 田中洋枝、柴崎康宏、藪健史、森友忠昭、中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 海洋研究開発機構
  • Year and Date: 2011-08-21
• [Presentation] コイTPOの遺伝子クローニングおよび性状解析 (2011)
  • Author(s): 林潔高、片倉文彦、藪健史、森友忠昭、中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 海洋研究開発機構
  • Year and Date: 2011-08-21
• [Presentation] In vitroにおけるコイCD4+T細胞のクローン性増殖 (2011)
  • Author(s): 山口卓哉・片倉文彦・森友忠昭・中西照幸
  • Organizer: 日本生体防御学会
  • Place of Presentation: 那覇市ぶんかテンブス館
  • Year and Date: 2011-06-29
• [Presentation] In vitroにおけるコイCD4+T細胞のクローン性増殖 (2011)
  • Author(s): 山口卓哉、片倉文彦、森友忠昭、中西照幸
  • Organizer: 日本生体防御学会
  • Place of Presentation: 那覇市ぶんかテンブス館テンブスホール
  • Year and Date: 2011-06-29
• [Presentation] Clonal growth of Carp (Cyprinus carpio) helper T-cells (2011)
  • Author(s): Tadaaki Moritomo
  • Organizer: 2012 Taiwan-Japan Symposium on Emerging Trends in Aquatic Animal Immunity and Aquaculture Biotechnology
  • Place of Presentation: National Cheng Kung University
  • Year and Date: 2011-02-21
• [Presentation] 魚類の造血幹/前駆細胞の分離と同定 (2011)
  • Author(s): 森友忠昭
  • Organizer: 日本大学幹細胞研究フォーラム
  • Place of Presentation: 日本大学会館
  • Year and Date: 2011-01-22
• [Presentation] Clonal growth of carp(Cyprinus carpio) CD4 single-positive αβT cells in vitro (2010)
  • Author(s): Takuya Yamaguchi, Fumihiko Katakura, Tadaaki Moritomo
  • Organizer: Sixth International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, Florida, USA
  • Year and Date: 2010-09-05
• [Presentation] Clonal growth of carp (Cyprinus carpio) CD4 single-positive α β T cells in vitro (2010)
  • Author(s): T. Yamaguchi, F. Katakura, T.Moritomo
  • Organizer: 6^<th> international symposium on aquatic animal health
  • Place of Presentation: Tampa, Florida USA
  • Year and Date: 2010-09-01
• [Presentation] コイ末梢血白血球の培養におけるBリンパ球の増殖 (2010)
  • Author(s): 仁井田祥光・片倉文彦・山口卓哉・森友忠昭・中西照幸
  • Place of Presentation: 九州大学西新プラザ第会議室
  • Year and Date: 2010-08-02
• [Presentation] ギンブナにおけるCD4, CD8陽性T細胞サブセットの組織分布 (2010)
  • Author(s): 小川詩乃、戸田秀明、柴崎康宏、森友忠昭、中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-08-01
• [Presentation] コイ末梢血白血球の培養におけるBリンパ球の増殖 (2010)
  • Author(s): 仁井田祥光, 片倉文彦, 山口卓哉, 森友忠昭, 中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 九州大学
  • Year and Date: 2010-08-01
• [Presentation] クローンギンブナを用いたエピジェネティクス研究モデルの構築 (2010)
  • Author(s): 森友忠昭・伊佐拓也・大野早苗
  • Organizer: 日本大学先端バイオフォーラム
  • Place of Presentation: 日本大学会館
  • Year and Date: 2010-02-23
• [Presentation] 魚類好中球における活性酸素産生とNADPHoxidase遺伝子の発現 (2009)
  • Author(s): 川口祐紀・村田学博・森友忠昭・中西照幸
  • Organizer: 日本水産学会
  • Place of Presentation: 盛岡地域交流センターマリオス
  • Year and Date: 2009-09-30
• [Presentation] コイ造血細胞の培養-増殖したT細胞のクローン性と分化段階の解析- (2009)
  • Author(s): 山口卓哉、片倉文彦、四反田聡、吉田美幸、森友忠昭、中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-08-03
• [Presentation] 魚類の造血幹/前駆細胞の分離と同定 (2009)
  • Author(s): 森友忠昭・関谷麻優子・小林功・片倉文彦・吉田美幸・山口卓哉・高野晃一
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-08-03
• [Presentation] ギンブナのアロ鱗移植部位におけるTリンパ球サブセットの動態 (2009)
  • Author(s): 今林望, 戸田秀明, 乙竹充, 森友忠昭, 中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-08-03
• [Presentation] コイ腎臓中の造血前駆細胞の同定 (2009)
  • Author(s): 片倉文彦・吉田美幸・山口卓哉・森友忠昭・中西照幸
  • Organizer: 日本比較免疫学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-08-03
• [Presentation] DEVELOPMENT OF THE SINGLE-CELL COLONY-FORMING ASSAY SYSTEM : CHARACTERIZATION OF CARP(CYPRINUS CARPIO)KIDNEY HEMATOPO IETIC PROGENITOR CELLS (2009)
  • Author(s): 片倉文彦・吉田美幸・山口卓哉・森友忠昭・中西照幸
  • Organizer: 11^<th> International society of developmental and comparative immunology
  • Place of Presentation: チェコ共和国プラハ
  • Year and Date: 2009-06-30
• [Presentation] LONG-TERM PROLIFERATION OF COMMON CARP(Cyprinus carpio)CD4+αβ T-CELLS IN CULTURE (2009)
  • Author(s): T.Yamaguchi, F.Katakura, M.Yoshida, M.Ohtani, T.Moritomo, T.Nakanish
  • Organizer: 11th International society of developmental and comparative immunology
  • Place of Presentation: チェコ共和国プラハ
  • Year and Date: 2009-06-30
• [Presentation] PERFORIN-DEPENDENT CYTOTOXIC MECHANISMS OF CD8 POSITIVE T CELLS IN GINBUNA CRUCIAN CARP (2009)
  • Author(s): H.TODA, M.OTOTAKEb, T.MORITOMO, T.NAKANISHI
  • Organizer: 11th International society of developmental and comparative immunology
  • Place of Presentation: チェコ共和国プラハ
  • Year and Date: 2009-06-30
• [Presentation] LONG-TERM PROLIFERATION OF COMMON CARP(Cyprinus carpio) CD4+αβT-CELLS IN CULTURE (2009)
  • Author(s): T. Yamaguchi, F. Katakura, M. Yoshida, M. Ohtani, T. Moritomo and T. Nakanishi
  • Organizer: 11^<th> International Society of Developmental and Comparative Immunology
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2009-06-27
• [Presentation] DEVELOPMENT OF THE SINGLE-CELL COLONY-FORMING ASSAY SYSTEM : CHARACTERIZATION OF CARP(CYPRINUS CARPIO) KIDNEY HEMATOPOIETIC PROGENITOR CELLS (2009)
  • Author(s): F. Katakura, M. Yoshida, T. Yamaguchi, T. Moritomo, and T. Nakanishi
  • Organizer: 11th International Society of Developmental and Comparative Immunology
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 2009-06-27
• [Book] 魚病学 (2012)
  • Author(s): 児玉洋・川本恵子・森友忠昭・和田新平
  • Total Pages: 150
  • Publisher: 緑書房
• [Remarks]
• [Remarks]
  • URL: http://hp.brs.nihon-u.ac.jp/~vetfish/start.htm
• [Remarks]
  • URL: http://hp.brs.nihon-u.ac.jp/~moritomo/index.htm