Functional analysis of implantation specific genes using RNA interference
| Project/Area Number |
21580349
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 着床 / インディアン・ヘッジホッグ(Ihh) / RNA干渉 / 子宮 / ラット / 受精胚 / インディアン・ヘッジホッグ / Foxa2 |
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| Research Abstract |
The present study was conducted to establish the RNA interference method for living rat uterus. We set up two implantation specific genes, Ihh and Foxa2, as a target gene for RNA interference. Firstly, expressional pattern and regulation mechanism was analyzed for these two genes. Based on the information, siRNA of both genes was introduced into the living rat uterus, and the control effect was verified by real-time RT-PCR. After 24h of the introduction, the expression level of genes was not different from the control groups. The results of the present study will be foundational information for establishing the RNA interference method in the living body uterus.
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Report
(4 results)
Research Products
(14 results)
