| Project/Area Number |
21590141
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental pharmacy
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| Research Institution | Osaka University of Pharmaceutical Sciences |
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Principal Investigator |
AMANO Fumio 大阪薬科大学, 薬学部, 教授 (90142132)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | サルモネラ / 乾燥耐性 / DPS(SEp22) / ストレス応答 / 病原因子 / Dps (SEp22) / Dps (Sep22) / Dps(Sep22) |
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| Research Abstract |
Mechanisms of dry-resistance by Salmonella Enteritidis(SE) were studied. Transfection of sep22-gene to sep22-deleted mutant cells recovered expression of SEp22 mRNA and the protein, together with ability to gain dry-resistance, showing that SEp22 is an essential factor for acquisition of dry-resistance. Analysis of nutrients in M9 medium necessary for SE to acquire dry-resistance revealed that both phosphate ion and glucose were essential. Besides, certain fractions separated from ingredients of casamino acid, common to LB medium, were shown to induce both SEp22 and acquisition of dry-resistance. Taken together, this study showed that SEp22 is an essential factor for SE to acquire dry-resistance, and nutritional factors, such as phosphate ion, glucose, and some ingredients in LB medium or casamino acid, are also involved in acquisition of dry-resistance.
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