Molecular mechanisms of postnatal development and topographical functional differentiation of the epididymis in mice.

• Project/Area Number: 21590193
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Chiba University
• Principal Investigator: KOMIYAMA Masatoshi 千葉大学, 大学院・医学研究院, 准教授 (70175339)
• Co-Investigator(Kenkyū-buntansha): MORI Chisato 千葉大学, 大学院・医学研究院, 教授 (90174375)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 発生学 / 形態形成学 / 精巣上体 / 遺伝子発現 / 発生 / 分化 / RT-PCR / マウス

Research Abstract

Gene expression was analyzed in mouse epididymis to clarify molecular mechanisms of topographical functional differentiation of the epididymis. Results were as follows ; 6 genes were identified as epididymis specific genes, 6 genes were identified as caput specific genes, 2 genes were identified as corpus specific genes, 1 gene was identified as a cauda specific gene, and 1 gene was identified as a caput and cauda specific gene.

Research Products

• [Journal Article] The changes of cortactin p80/85 isoform profiles and tyrosine phosphorylation status during spermatogenesis in the mouse testis (2010)
  • Author(s): Miyaso H, Komiyama M, Matsuno Y, Naito M, Hirai S, Itoh M Mori C
  • Journal Title: J. Androl Volume: Vol.31 Pages: 507-518
  • Peer Reviewed
• [Journal Article] The changes of cortactin p80/85 iso」orm profiles and tyrosine phosphory lation status during spermatogenesis in the mouse testis. (2010)
  • Author(s): Miyaso, H.
  • Journal Title: J.Andrology Volume: 31 Pages: 507-518
  • Peer Reviewed
• [Presentation] 新生児期デカブロモジフェニルエーテル投与がマウス精巣上体の精子数にあたえる影響 (2012)
  • Author(s): 宮宗秀伸, 中村典子, 松野義晴, 小宮山政敏, 落合伸伍, 井越有香, 森千里
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス
• [Presentation] The effects of neonatal exposure to deca-brominated diphenyl ether on spermatogenic cells in mouse testis (2012)
  • Author(s): Miyaso, H.
  • Organizer: The Society of Toxicology 51st Annual Meeting
  • Place of Presentation: San Francisco, USA
• [Presentation] デカブロモジフェニルエーテル投与がマウス精巣上体の精子数にあたえる影響 (2012)
  • Author(s): 宮宗秀伸
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 山梨大学(山梨)
• [Presentation] 新生児期デカブロモジフェニルエーテルが精子形成におよぼす影響 (2011)
  • Author(s): 宮宗秀伸
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: パシフィコ横浜(神奈川)(地震により会合は中止)
  • Year and Date: 2011-03-29
• [Presentation] The effects of neonatal exposure to deca-brominated diphenyl ether on apical ectoplasmic specialization in mouse testis. (2011)
  • Author(s): Miyaso, H.
  • Organizer: The Society of Toxicology 50_th Annual Meeting
  • Place of Presentation: Walter E.WashingtonConvention Center(Washington D.C., USA)
  • Year and Date: 2011-03-07
• [Presentation] Neonatal exposure to decabromodiphenyl ether disrupts apical ectoplasmic specialization in mouse testes by increasing cortactin tyrosine phosphorylation (2011)
  • Author(s): H. Miyaso, N. Nakamura, Y. Kawashiro, Y. Matsuno, M. Komiyama, S. Ochiai, Y. Igoshi C. Mori
  • Organizer: SSR 44th Annual Meeting
  • Place of Presentation: SSR 44th Annual Meeting
• [Presentation] デカブロモジフェニルエーテルがマウス精巣におけるコートアクチンに与える影響の解析 (2011)
  • Author(s): 宮宗秀伸, 中村典子, 松野義晴, 小宮山政敏, 落合伸伍, 井越有香, 森千里
  • Organizer: 日本アンドロロジー学会第30回学術大会および第17回精子形成・精巣毒性研究会 共同開催学会
  • Place of Presentation: 都市センターホテル、東京都千代田区
• [Presentation] デカブロモジフェニルエーテルがマウス精巣上体の遺伝子発現に与える影響 (2011)
  • Author(s): 中本真, 宮宗秀伸, 中村典子, 松野義晴, 小宮山政敏, 落合伸伍, 井越有香, 森千里
  • Organizer: 日本アンドロロジー学会第30回学術大会および第17回精子形成・精巣毒性研究会 共同開催学会
  • Place of Presentation: 都市センターホテル、東京都千代田区
• [Presentation] 新生児期デカブロモジフェニルエーテル投与が精子形成におよぼす影響 (2011)
  • Author(s): 宮宗秀伸, 松野義晴, 小宮山政敏, 落合伸伍, 井越有香, 森千里
  • Organizer: 第88回日本生理学会大会および第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: パシフィコ横浜・会議センター、神奈川県横浜市
• [Presentation] The effects of neonatal exposure to decabrominated diphenyl ether on apical ectoplasmic specialization in mouse testis (2011)
  • Author(s): H. Miyaso, Y. Matsuno, M. Komiyama, S. Ochiai, Y. Igoshi C. Mori
  • Organizer: Society of Toxicology 50th Anniversary Annual Meeting
  • Place of Presentation: Washington D.C., USA
• [Presentation] デカブロモジフェニルエーテルがマウス精巣におけるコートアクチンに与える影響の解析 (2011)
  • Author(s): 宮宗秀伸
  • Organizer: 日本アンドロロジー学会第30回学術大会
  • Place of Presentation: 都市センターホテル(東京)
• [Presentation] デカブロモジフェニルェーテルがマウス精巣上体の遺伝子発現に与える影響 (2011)
  • Author(s): 中本真
  • Organizer: 日本アンドロロジー学会第30回学術大会
  • Place of Presentation: 都市センターホテル(東京)
• [Presentation] Neonatal exposure to decabromodiphenyl ether disrupts apical ectoplasmic specialization in mouse testes by increasing cortactin tyrosine phosphorylation (2011)
  • Author(s): Miyaso, H.
  • Organizer: SSR 44th Annual Meeting
  • Place of Presentation: Portland, Oregon, USA
• [Presentation] 新生児期デカブロモジフェニルエーテルがマウス精巣に与える影響 (2010)
  • Author(s): 宮宗秀伸
  • Organizer: 環境ホルモン学会第13回研究発表会
  • Place of Presentation: 東京大学山上会館(東京
  • Year and Date: 2010-12-17