Morphological and molecular backgrounds for localization of ATP-induced signals in glial cells associated with cutaneous sensory devices.

• Project/Area Number: 21590206
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Hokkaido University
• Principal Investigator: TAKAHASHI-IWANAGA Hiromi 北海道大学, 大学院・医学研究科, 准教授 (30193759)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 感覚神経終末 / 終末シュワン細胞 / カベオラ / カルシウム画像 / アデノシン5'三リン酸 / グリア / アデノシン5'-三リン酸 / プリン作動性信号 / G蛋白共役型受容体 / 免疫組織化学

Research Abstract

Glial cells in cutaneous sensory devices extend multiple lamellar processes that cover different axon terminals and generate their own Ca^2+ signals in response to stimulation with the neurotransmitter ATP. We examined links between this glial signaling and the plasma membrane microdomains caveolae by immunohistochemistry and Ca^2+ image analysis in isolated lanceolate sensory endings. motion detectors of rat vibrissae. Our data have shown that caveola-dependent localization of the ATP receptor P2Y_2 and its coupling partner G_q/11 to the glial lamellae is essential for the subcellular compartmentalization of the signals.

Research Products

• [Journal Article] Accumulated caveolae constitute sub cellular compartments for glial calcium signaling in lanceolate sensory endings innervating rat vibrissae (2012)
  • Author(s): Takahashi-Iwanaga H, Iwanaga T
  • Journal Title: J Comp Neurol Volume: 520 Pages: 2053-2066
  • Peer Reviewed
• [Journal Article] Accumulated caveolae constitute subcellular compartments for glial calcium signaling in lanceolate sensory endings innervatine rat (2012)
  • Author(s): Takahashi-Iwanaga H, Iwanaga T
  • Journal Title: J Comp Neurol Volume: (印刷中) Issue: 9 Pages: 2053-2066
  • DOI: 10.1002/cne.23028
  • Peer Reviewed
• [Journal Article] 知覚終末グリア細胞のプリン作動性信号系 (2011)
  • Author(s): 岩永ひろみ
  • Journal Title: 顕微鏡 Volume: 46 Pages: 217-221
  • Peer Reviewed
• [Journal Article] Intensified expressions of a nomocaroxylate transporter in consistently renewing tissues of the mouse (2011)
  • Author(s): Takebe K, Takahashi-Iwanaga H, Iwanaga T
  • Journal Title: Biomed Res Volume: 32 Pages: 293-301
  • Peer Reviewed
• [Journal Article] Immunohistochemical demonstration of dopamine receptor D2R in the primary cilia of the mouse pituitary gland (2011)
  • Author(s): Iwanaga T, Hozumi Y, Takahashi-Iwanaga H
  • Journal Title: Biomed Res Volume: 32 Pages: 225-235
  • Peer Reviewed
• [Journal Article] Restricted expression of somatostatin receptor 3 to primary cilia in the pancreatic islets and adenohypophysis of mice (2011)
  • Author(s): Iwanaga T, Miki T, Takahashi-Iwanaga H
  • Journal Title: Biomed Res Volume: 32 Pages: 73-81
  • Peer Reviewed
• [Journal Article] Cellular expression of monocaroxylate transporters in the female reproductive organ of mice : implications for the genital lactate shuttle (2011)
  • Author(s): Kuchiiwa T, Nio-Koayashi J, Takahashi-Iwanaga H, Yajima T, Iwanaga T
  • Journal Title: Histochem Cell Biol Volume: 135 Pages: 351-360
  • Peer Reviewed
• [Journal Article] Functional characterization of a ClC-2-like Cl-conductance in surface epithelial cells of rat rectal colon (2011)
  • Author(s): Inagaki A, Yamaguchi S, Takahashi-Iwanaga H, Iwanaga T, Ishikawa T
  • Journal Title: J Membr Biol Volume: 235 Pages: 27-41
  • Peer Reviewed
• [Journal Article] Cellular expression of monocarbocylate transporters in the female re-productive organ of mice: implications fro the genital lactate shuttle. (2011)
  • Author(s): Kuchiiwa, T., et al.
  • Journal Title: Histochem. Cell Biol. Volume: 135 Pages: 351-360
  • Peer Reviewed
• [Journal Article] Restricted expression of somatostatin receptor 3 to primary cilia in the pancreatic islets and adenohvnonhvsis of mice. (2011)
  • Author(s): Iwanaga, T., et al.
  • Journal Title: Biomed. Res. Volume: 32 Pages: 73-81
  • Peer Reviewed
• [Journal Article] Cellular expression of the monocaroxylate transporter (MCT) family in the placenta of mice (2010)
  • Author(s): Nagai A, Takebe K, Nio-Kobayashi J, Takahashi-Iwanaga H, Iwanaga T
  • Journal Title: Placenta Volume: 31 Pages: 126-133
  • Peer Reviewed
• [Journal Article] Functional characterization of a CIC-2-like Cl conductance in surface epithelial cells of rat rectal colon. (2010)
  • Author(s): Inagaki, A., et al.
  • Journal Title: J. Membr. Biol. Volume: 235 Pages: 27-41
  • Peer Reviewed
• [Journal Article] Cellular expression of the monocarboxylate transporter(MCD)family in the placenta of mice. (2010)
  • Author(s): Nagai, A., et al.
  • Journal Title: Placenta 31 Pages: 126-133
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Baron, A.
  • Journal Title: Glial Cells in Health and Disease(Medimond, Bologna) Pages: 171-174
• [Journal Article] Rgulation of hair shedding by the type 3 inositol 1, 4, 5-trisphophate receptor
  • Author(s): Sato-Miyaoka M, Hisatsune C, Ebisui E, Ogawa N, Takahashi-Iwanaga H, Mikoshiba K
  • Journal Title: J Invest Dermatol Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] lation of hair shedding by the type 3 inositol 1,4,5-trisphophate recept
  • Author(s): Sato-Miyaoka M, Hisatsune C, Ebisui E, Ogawa N, Takahashi-
  • Journal Title: J Invest Dermatol Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Purinergic signaling in glial cells associated with cutaneous mechano-receptors (2011)
  • Author(s): Takahashi-Iwanaga H
  • Organizer: 第116回解剖学会総会・全国学術総会
  • Place of Presentation: J. Phyiol. Sci. 誌上開催(関東・東北大震災のため)
  • Year and Date: 2011-03-28
• [Presentation] Purinergic signaling in glial cells associated with cutaneous mechano-receptors. (2011)
  • Author(s): Takahashi-Iwanaga, H.
  • Organizer: 第116回解剖学会総会・全国学術集会
  • Place of Presentation: 関東・東北大震災のためJ.Physiol.Sci.誌上開催
  • Year and Date: 2011-03-28
• [Presentation] Accumulated caveolae constitute sucellular compartments of calcium signaling in the lamellar Schwann cells associated with cutaneous mechanoreceptors (2010)
  • Author(s): Takahashi-Iwanaga H
  • Organizer: The 21st International Symposium on Morphological Sciences
  • Place of Presentation: Tormina, Italy
  • Year and Date: 2010-09-19
• [Presentation] Accumulated caveolae constitute subcellular compartments of calcium signaling in the lamellar Schwann cells associated with cutaneous mechanoreceptors (2010)
  • Author(s): Takahashi-Iwanaga, H.
  • Organizer: The 21st International Symposium on Morphological Sciences
  • Place of Presentation: サンドメニコパレスホラル,Taormina,Italy 招待講演
  • Year and Date: 2010-09-19
• [Presentation] 槍型知覚終末におけるグリア性カルシウム信号の細胞内区域化機構,とくにカベオラの役割について (2010)
  • Author(s): 岩永ひろみ
  • Organizer: 第115回解剖学会総会・全国学術集会
  • Place of Presentation: 盛岡,岩手県民会館
  • Year and Date: 2010-03-30
• [Presentation] Purinergic signaling in terminal Schwann cells associated with lanceolate sensory endings (2009)
  • Author(s): Takahashi-Iwanaga H
  • Organizer: The 9th European Meeting on Glial Cells
  • Place of Presentation: Paris,France
  • Year and Date: 2009-09-09
• [Presentation] Purinergic signaling in terminal Schwann cells associated with lanceolate sensory endings. (2009)
  • Author(s): Takahashi-Iwanaga, H.
  • Organizer: 9th European Meeting on Glial Cells
  • Place of Presentation: Paris,デカルト大学
  • Year and Date: 2009-09-09
• [Presentation] Clustered caveolae support initiation and propagation of local purinergic signals in glial processes accompanying lanceolate sensory endings (2009)
  • Author(s): Takahashi-Iwanaga H
  • Organizer: International Symposium on Purinergic Signaling in New Strategy of Drug Discovery
  • Place of Presentation: 福岡,ホテル・ザ・ルイガンス
  • Year and Date: 2009-07-24
• [Presentation] Clustered caveolae support initiation and propagation of local purinergic signals in glial processes accompanying lanceolate sensory endings. (2009)
  • Author(s): Takahashi-Iwanaga, H.
  • Organizer: International Symposium on Purinergic Signaling in New Strategy of Drug Discovery
  • Place of Presentation: 福岡,ホテルザルイガンス
  • Year and Date: 2009-07-24
• [Remarks]
  • URL: http://www.med.hokudai.ac.jp/~anat-3w/kenkyu.htm