High throughput screening for G protein-coupled receptor(s) activated in response to estrogen by RNAi collection
Project/Area Number |
21590256
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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Research Institution | Yamaguchi University |
Principal Investigator |
MIZUKAMI Yoichi 山口大学, 総合科学実験センター, 教授 (80274158)
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Co-Investigator(Kenkyū-buntansha) |
YOKOMIZO Takehiko 九州大学, 医学研究院, 教授 (60302840)
岡本 まり子 山口大学, 総合科学実験センター, 助教 (30415111)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 女性ホルモン / 細胞膜受容体 / G蛋白結合型受容体 / RNAi / スクリーニング / エストロゲン / エストエゲン / GPCR / シグナル伝達 / 受容体 / 生殖器 |
Research Abstract |
Estrogen induces the increase of intracellular Ca^<2+> concentrations in addition to transcriptional activity via nuclear receptor, but the membrane receptor except for GPR30 remains unknown. We have produced RNAi collection for GPCR, and examined the Ca^<2+> response and cell impedance in response to estrogen in the presence of RNAi collection. The membrane receptor did not be identified under the experimental conditions due to the weak response of estrogen. The other assay system will be requested to elucidate the estrogen membrane receptor.
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Optimizing high-resolution melting analysis for the detection of mutations of GPR30/ GPER-1 in breast cancer2012
Author(s)
Aihara, M., Yamamoto, S., Nishioka, H., Hamano, K., Oka, M., and Mizukami, Y.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] A high-affinity monoclonal antibody against the FLAG tag useful for G-protein coupled receptor study2012
Author(s)
Sasaki, F., Okuno, T., Saeki, K., Min, L., Onohara, N., Kato, H., Shimizu, T. and Yokomizo, T.
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Journal Title
Anal. Biochem
Volume: (in press)
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Peer Reviewed
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[Journal Article] Optimizing high-resolution melting analysis for the detection of mutations of GPR30/GPER-1 in breast cancer2012
Author(s)
Aihara, M., Yamamoto, S., Nishioka, H., Hamano, K., Oka, M., Mizukami, Y.
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Journal Title
Gene
Volume: (in press)
Issue: 2
Pages: 95-105
DOI
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Peer Reviewed
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[Journal Article] International Union of Basic and Clinical Pharmacology. LXXXIV : Leukotriene Receptor Nomenclature, Distribution, and Pathophysiological Functions2011
Author(s)
Back, M., Dahlen, S. E., Drazen, J. M., Evans, J. F., Serhan, C. N., Shimizu, T., Yokomizo, T. and Rovati, G. E.
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Journal Title
Pharmacol
Volume: 63
Pages: 539-584
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Peer Reviewed
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[Journal Article] Operative injury accelerates tumor growth by inducing mobilization and recruitment of bone marrow-derived stem cells.2011
Author(s)
Takemoto Y, Li TS, Kubo M, Ohshima M, Ueda K, Harada E, Enoki T, Okamoto M, Mizukami Y, Murata T, Hamano K.
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Journal Title
Surgery
Volume: 149(6)
Pages: 792-800
Related Report
Peer Reviewed
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[Journal Article] Operative injury accelerates tumor growth by inducing mobilization and recruitment of bone marrow-derived stem cells2011
Author(s)
Takemoto Y, Li TS, Kubo M, Ohshima H, Ueda K, Harada E, Enoki T, Okamoto M, Mizukami Y, Murata T, Hamano K
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Journal Title
Surgery
Volume: Jun;149(6)
Issue: 6
Pages: 792-800
DOI
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Peer Reviewed
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[Journal Article] Alterations in adhesion junction precede gap junction remodeling during the development of heart failure in cardiomyopathic hamster2011
Author(s)
Yoshida M, Ohkusa T, Nakashima T, Takanari H, Yano M, Takemura G, Honjo H, Kodama I, Mizukami Y, Matsuzaki, M
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Journal Title
Cardiovasc.Res
Volume: Oct 1;92(1)
Issue: 1
Pages: 95-105
DOI
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Peer Reviewed
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[Journal Article] Proteomic Analysis of Proteins Expressing in Regions of Rat Brain by Combination of SDS-PAGE with Nano-Liquid Chromatography-Quadrupole Tandem Mass spectrometry2010
Author(s)
Katagiri, T., Hatano, N., Aihara, M., Kawano, H., Okamoto, M., Xing, L., Izumi, T., Maekawa, T., Nakamura, S., Ishihara, T., Shirai, M., Mizukami, Y.
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Journal Title
Proteome Science
Volume: 8(1): 41
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[Journal Article] Neurtrophil elastase inhibitor, ONO-5046, attenuates hippocampal neuronal damage after transient forebrain ischemia in rats2009
Author(s)
Matayoshi, H., Hirata, T., Yamashita S., Ishida, K., Mizukami, Y., Gondo, T., Matsumoto, M., Sakabe, T.
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Journal Title
Brain Res
Volume: 1259
Pages: 98-106
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Peer Reviewed
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[Journal Article] Repeated preconditioning with hyperbaric oxygen induces neuroprotection against forebrain ischemia via suppression of p38 mitogen activated protein kinase2009
Author(s)
Yamashita, S., Hirata, T., Mizukami, Y. Cui, Y. J., Fukuda, S., Ishida, K., Matsumoto, M., Sakabe, T.
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Journal Title
Brain Res.
Volume: 1301
Pages: 171-179
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Peer Reviewed
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[Presentation] Arrest of spermatogenesis at round spermatids in PLCZ1-deficient mice.2010
Author(s)
Masahiko Ito, Kentaro Nagaoka, Keiji Kuroda, Natsuko Kawano, Keiichi, Yoshida, Yuichirou Harada, Tomohide Shikano, Mami Miyado, Shoji Oda, Kiyotaka Toshimori, Youichi Mizukami, Tomoaki Murata, Akihiro Umezawa, Shunichi Miyazaki, Kenji Miyado.
Organizer
11th International Symposium on Spermatology
Place of Presentation
Okinawa, Japan conbention center
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