Development of novel strategy for the treatment of inflammatory diseases with natural products such as herbal ingredients

• Project/Area Number: 21590278
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Nagoya University
• Principal Investigator: ISHIGURO Kazuhiro 名古屋大学, 大学院・医学系研究科, 講座准教授 (60432275)
• Co-Investigator(Kenkyū-buntansha): ANDO Takafumi 名古屋大学, 大学院・医学系研究科, 准教授 (80378041) ; GOTO Hidemi 名古屋大学, 大学院・医学系研究科, 教授 (10215501)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 炎症 / 腸炎 / 天然化合物 / 生薬 / マクロファージ / T細胞 / lipopolysaccharide / サイトカイン

Research Abstract

The action and therapeutic benefit of Dehydrocorydaline were demonstrated.
Atractylodin was found to inhibit IL-17production in activated T cells.
The molecular mechanism of acetate action on Tcell activation was elucidated.
The novel colitis model for fluorescence observation of hapten-protein was developed.
The novel agent for fluorescence observation of colitis was developed.

Research Products

• [Journal Article] De hydrocorydaline inhibits elevated mitochondrial membrane potential in lipopolysaccharide-stimulated macrophages (2011)
  • Author(s): Kazuhiro Ishiguro, Takafumi Ando, Osamu Maeda, Osamu Watanabe, Hidemi Goto
  • Journal Title: Int. Immunopharmacol Volume: 11 Pages: 1362-1367
  • Peer Reviewed
• [Journal Article] Tubulin alpha functions as an adaptor for NFAT-importin beta interaction (2011)
  • Author(s): Kazuhiro Ishiguro, Takafumi Ando, Osamu Maeda, Osamu Watanabe, Hidemi Goto
  • Journal Title: J. Immunol Volume: 186 Pages: 2710-2713
  • Peer Reviewed
• [Journal Article] Dehydrocorydaline inhibits elevated mitochondrial membrane potential in lipopolysaccharide-stimulated macrophages (2011)
  • Author(s): 石黒和博, ら
  • Journal Title: International Immunopharmacology Volume: 11 Issue: 9 Pages: 1362-7
  • DOI: 10.1016/j.intimp.2011.04.022
  • Peer Reviewed
• [Journal Article] Tubulin α functions as an adaptor for NFAT-importin β interaction (2011)
  • Author(s): 石黒和博, ら
  • Journal Title: Journal of Immunology Volume: 186 Issue: 5 Pages: 2710-3
  • DOI: 10.4049/jimmunol.1003322
  • Peer Reviewed
• [Journal Article] Novel mouse model of colitis characterized by hapten-protein visualization (2010)
  • Author(s): Kazuhiro Ishiguro, Takafumi Ando, Osamu Maeda, Osamu Watanabe, Hidemi Goto
  • Journal Title: Biotechniques Volume: 49 Pages: 641-648
  • Peer Reviewed
• [Journal Article] Novel application of low pH-dependent fluorescent dyes to examine colitis (2010)
  • Author(s): Kazuhiro Ishiguro, Takafumi Ando, Osamu Watanabe, Hidemi Goto
  • Journal Title: BMC Gastroenterol Volume: 10 Pages: 4-4
  • Peer Reviewed
• [Journal Article] Novel application of low pH-dependent fluorescent dyes to examine colitis (2010)
  • Author(s): 石黒和博, ら
  • Journal Title: BMC Gastroenterology 10 Pages: 4-4
  • Peer Reviewed
• [Presentation] ハプテン-タンパク複合体を蛍光観察できる新しい腸炎モデルの開発 (2011)
  • Author(s): 石黒和博
  • Organizer: 第48回日本消化器免疫学会総会
  • Place of Presentation: 石川県金沢市
  • Year and Date: 2011-07-21
• [Presentation] ハプテンータンパク複合体を蛍光観察できる新しい腸炎モデルの開発 (2011)
  • Author(s): 石黒和博, ら
  • Organizer: 第48回日本消化器免疫学会総会
  • Place of Presentation: 石川県金沢市
  • Year and Date: 2011-07-21
• [Presentation] 大腸粘膜蛍光観察検査試薬の開発 (2010)
  • Author(s): 石黒和博
  • Organizer: 第47回日本消化器免疫学会総会
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2010-07-08
• [Presentation] 大腸粘膜蛍光観察検査試薬の開発 (2010)
  • Author(s): 石黒和博, ら
  • Organizer: 第47回日本消化器免疫学会総会
  • Place of Presentation: 滋賀県大津市大津プリンスホテル
  • Year and Date: 2010-07-08
• [Presentation] 大腸粘膜の腺管および炎症細胞の新しい蛍光観察法 (2009)
  • Author(s): 石黒和博
  • Organizer: JDDW 2009シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2009-10-15
• [Presentation] 大腸粘膜の腺管および炎症細胞の新しい蛍光観察法 (2009)
  • Author(s): 石黒和博, ら
  • Organizer: JDDW 2009 シンポジウム
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2009-10-15
• [Remarks] English website
  • URL: http://www.med.nagoya-u.ac.jp/english01/3189/gastroenterology.html
• [Remarks] Japanese website
  • URL: http://www.med.nagoya-u.ac.jp/medical/1640/shoukakishikkanbyoutairon.html
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/english01/3189/gastroenterology.html
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/medical/1640/shoukakishikkanbyoutairon.html
• [Remarks] English
  • URL: http://www.med.nagova-u.ac.jp/medical_en_test/3189/gastroenterology.html
• [Remarks] Japanese
  • URL: http://www.med.nagova-u.ac.jp/medical/1640/shoukakishikkanbyoutairon.html
• [Remarks]
  • URL: http://kenpro.mynu.jp:8001/Profiles/0050/0005087/profile.html
• [Remarks]
  • URL: http://www.med.nagoya-u.ac.jp/medical/102/e10207.html
• [Patent(Industrial Property Rights)] 腸管粘膜蛍光観察用検査試薬 (2010)
  • Inventor(s): 石黒和博
  • Industrial Property Rights Holder: 名古屋大学
  • Filing Date: 2010-08-02
  • Overseas
• [Patent(Industrial Property Rights)] 活性化マクロファージ特異的細胞死誘導薬 (2009)
  • Inventor(s): 石黒和博, ら
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Number: 2009-284267
  • Filing Date: 2009-12-22
• [Patent(Industrial Property Rights)] 活性化マクロファージ特異的細胞死誘導薬 (2008)
  • Inventor(s): 石黒和博
  • Industrial Property Rights Holder: 名古屋大学
  • Filing Date: 2008-12-22