Studies on molecular basis for regulation of beta-oxidation system

• Project/Area Number: 21590314
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Fukushima Medical University
• Principal Investigator: HOMMA Yoshimi 福島県立医科大学, 医学部, 教授 (60192324)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: リン酸化 / シグナル / ミトコンドリア / デヒドロゲナーゼ / 脂肪酸 / 過酸化脂質

Research Abstract

Phosphorylation level of VLCAD1 decreased in mitochondria of lung fibroblasts established from patients with idiopathic pulmonary lung fibrosis. PKA phosphorylated VLCAD1 at Ser-586, and its phosphorylation-defective mutant enhanced production of radical oxygen species. These suggest that beta oxidation system is regulated by intra-mitochondrial signaling system including PKA.

Research Products

• [Journal Article] Coronary vasospasm induced in transgenic mouse with the increased phospholipase C-δ1 activity (2012)
  • Author(s): Shibutani S, et al
  • Journal Title: Circulation Volume: 125 Pages: 1027-36
  • Peer Reviewed
• [Journal Article] CpG methylation prevents YY1-mediated transcriptional activation of the vimentin promoter (2011)
  • Author(s): Sekimata M, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 414 Pages: 767-72
  • Peer Reviewed
• [Journal Article] Dysregulation of very long chain acyl-CoA dehydrogenase coupled with lipid peroxidation (2010)
  • Author(s): Kabuyama Y, Suzuki T, Nakazawa N, Yamaki J, Homma MK, Homma Y
  • Journal Title: Am J Physiol Cell Physiol Volume: 298巻
  • Peer Reviewed
• [Journal Article] p122 protein enhances intracellular calcium increase to acetylcholine. Its possible role in the pathogenesis of coronary spastic angina (2010)
  • Author(s): Murakami R, Osanai T, Tomita H, Sasaki S, Maruyama A, Itoh K, Homma Y, Okumura K
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 30巻 Pages: 1968-1975
  • Peer Reviewed
• [Journal Article] Dysregulation of very long chain acyl-CoA dehydrogenase coupled with lipid peroxydation. (2009)
  • Author(s): Kabuyama Y, et al.
  • Journal Title: Am J Physiol Cell Physiol 298
  • Peer Reviewed
• [Presentation] Identification of autophosphorylation residues in CK2 by mass spectrometric analysis (2011)
  • Author(s): 本間美和子, ほか
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] ミトコンドリアc-Srcキナーゼ活性を制御する新規相互作用タンパク質の同定と機能解析 (2011)
  • Author(s): 小椋正人, 八巻淳子, 本間美和子, 本間好
  • Organizer: 84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] ミトコンドリアc-Srcキナーゼ活性を制御する新規相互作用タンパク質の同定と機能解析 (2011)
  • Author(s): 小椋正人, ほか
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] Src regulates mitochondria function and ROS generation through direct phosphorylation of respiration components (2011)
  • Author(s): Homma Y, Ogura M, Homma MK
  • Organizer: Charles Heidelberger International Symposium on Cancer Research
  • Place of Presentation: 中国西安
  • Year and Date: 2011-06-06
• [Presentation] Src regulates mitochondria function and ROS generation through direct phosphorylation of respiratory components (2011)
  • Author(s): Homma Y, et al
  • Organizer: The 17^<th> International Charles Heidelberger Symposium on Cancer Research
  • Place of Presentation: Xian, China
  • Year and Date: 2011-06-06
• [Presentation] Reguratory mechanism of oxidative phosphorylation by mitochondrial c-Src (2011)
  • Author(s): Ogura M, Yamaki J, Homma MK, Homma Y
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: 米国キーストーン
  • Year and Date: 2011-01-26
• [Presentation] Regulation of oxidative phosphorylation by mitochondrial c-Src (2010)
  • Author(s): 小椋正人, 八巻淳子, 本間美和子, 本間好
  • Organizer: 83回日本生化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-10
• [Presentation] Regulation of oxidative phosphorylation of mitochondrial c-Src (2010)
  • Author(s): 小椋正人, ら
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-10
• [Presentation] Autophosphorylation of CK2 N-terminal region controls activation and nuclear localization during the progression of cell cycle (2010)
  • Author(s): 本間美和子, ら
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-10
• [Presentation] ROS production in lung fibrosis myofibroblasts (2010)
  • Author(s): Homma Y
  • Organizer: Charles Heidelberger International Symposium on Cancer Research
  • Place of Presentation: タイ国ピサヌローク
  • Year and Date: 2010-01-18
• [Presentation] ROS production in lung fibrosis myofibroblasts. (2010)
  • Author(s): Homma, Y
  • Organizer: 15^<th> Charles Heidelberger International Symposium on Cancer Research
  • Place of Presentation: タイ、ピサヌローク市
  • Year and Date: 2010-01-18
• [Presentation] 肺線維芽細胞のプロテオミクス解析 (2009)
  • Author(s): 鈴木俊幸, 蕪山由己人, 八巻淳子, 本間美和子, 本間好
  • Organizer: 82回日本生化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2009-12-22
• [Presentation] 肺線維芽細胞のプロテオミクス解析 (2009)
  • Author(s): 鈴木俊幸, ら
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸市
  • Year and Date: 2009-10-22
• [Remarks]
  • URL: http://www.fmu.ac.jp/home/biomol/html/index.html
• [Remarks]
  • URL: http://www.fmu.ac.jp/home/biomol/HTML/index.html