Phosphoprotein array analyses for determination of signaling pathways activated by p63 in squamous cell carcinomas

• Project/Area Number: 21590434
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: University of Yamanashi
• Principal Investigator: KATOH Iyoko 山梨大学, 医学工学総合研究部, 准教授 (20333297)
• Co-Investigator(Renkei-kenkyūsha): KURATA Shunichi 東京医科歯科大学, 難治疾患研究所, 准教授 (60140901)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 腫瘍 / p63 / PP2A / TCF-4 / Wnt / 癌 / シゲナル伝達 / 発現制御 / 遺伝子 / マイクロアレイ / シグナル伝達 / プロテオーム

Research Abstract

To explore p63-controlled cellular signaling pathways, we performed p63 RNA interference, phosphoprotein array analyses and gene expression profiling. Results suggest that the.ΔNp63αisoform interacts with protein phosphatase 2A(PP2A),β-catenin and TCF-4 to activate Wnt signal target gene expression.

Research Products

• [Journal Article] Activation of the long terminal repeat of human endogenous retrovirus K by melanoma-specific transcription factor MITF-M (2011)
  • Author(s): Katoh I, Mirova A, Kurata S, Murakami Y, Horikawa K, Nakakuki N, Sakai T, Hashimoto K, Maruyama A, Yonaga T, Fukunishi N, Moriishi K, Hirai H.
  • Journal Title: Neoplasia Volume: 13 Issue: 11 Pages: 1081-1092
  • DOI: 10.1593/neo.11794
  • Peer Reviewed
• [Journal Article] Induction of DeltaNp63 by the eratinocyte-specipicpathway of TGF-beta with Smad2 and I kappa B kinase alpha in squamous cell carcinoma (2011)
  • Author(s): Fukunish N, Katoh I, Tomimori T, Tsukinoki K, Hata RI, Nakao A, Ikawa Y, Kurata S
  • Journal Title: Neoplasia Volume: 12 Issue: 12 Pages: 969-979
  • DOI: 10.1593/neo.101054
  • Peer Reviewed
• [Journal Article] Lung. lung interaction in isolated perfused unilateral hyperventilated rat lungs (2010)
  • Author(s): Bilali A, Kurata S, Ikeda S, Georgieva GS, Zhu C, Tomita M, Katoh I, Mitaka C, Eishi Y, Imai T.
  • Journal Title: Translational Research Volume: 155 Issue: 5 Pages: 228-237
  • DOI: 10.1016/j.trsl.2010.01.001
  • Peer Reviewed
• [Journal Article] Induction of DeltaNp63 by the keratinocyte-specipic pathway of TGF-beta with Smad2 and I kappa B kinase alpha in squamous cell carcinoma (2010)
  • Author(s): Fukunishi N.
  • Journal Title: Neoplasia Volume: 12 Pages: 969-979
  • Peer Reviewed
• [Journal Article] Lung-lung interaction in isolated perfused unilateral hyperventilated rat lungs (2010)
  • Author(s): Bilali A, (et al.)
  • Journal Title: Translational Research 155 Pages: 228-237
  • Peer Reviewed
• [Presentation] 扁平上皮癌でのp63による核内GSK-3βを介したβカテニンの制御 (2012)
  • Author(s): 加藤伊陽子
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2012-10-03
• [Presentation] 扁平上皮癌でのp63による核内GSK-3βを介したβカテニンの制御 (2012)
  • Author(s): 加藤伊陽子
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2012-10-03
• [Presentation] p63による核内GSK-3βを介したβ-catenin依存性遺伝子発現の制御 (2012)
  • Author(s): 倉田俊一
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2012-09-22
• [Presentation] p63による核内GSK-3βを介したβ-catenin依存性遺伝子発現の制御 (2012)
  • Author(s): 倉田俊一
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2012-09-22
• [Presentation] Human Endogenous Retrovirus K (HERV-K) is induced by MITF-M, amelanogenesis transcription factor activated in melanomas (2012)
  • Author(s): Katoh I
  • Organizer: American Association for Microbiology General Meeting 2011
  • Place of Presentation: Ernest Morial Convention Center (New Orleans, USA)
  • Year and Date: 2012-05-22
• [Presentation] Human Endogenous Retrovirus K(HERV-K) is induced by MITF-M, a melanogenesis transcription factor activated in melanomas (2011)
  • Author(s): Katoh, I
  • Organizer: American Association for Microbiology General Meeting 2011
  • Place of Presentation: Ernest Morial Convention Center(New Orleans, USA)
  • Year and Date: 2011-05-22
• [Presentation] Induction of p63 by the keratinocyte-specificTGF-beta signaling pathway in squamous cell carcinomas (2010)
  • Author(s): 福西菜穂子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-09-23
• [Presentation] Induction of p63 by the keratinocyte-specific TGF-beta signaling pathway in squamous cell carcinomas. (2010)
  • Author(s): 福西菜穂子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-23
• [Presentation] Induction of human endogenous retrovirus-K(HERV-K) LTR in melanomas by MITF, a malanogenesis transcription factor (2010)
  • Author(s): 加藤伊陽子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2010-09-22
• [Presentation] Induction of human endogenous retrovirus-K(HERV-K)LTR in melanomas by MITF, a melanogenesis transcription factor. (2010)
  • Author(s): 加藤伊陽子
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-22
• [Presentation] p63のΔNプロモータはケラチノサイト特異的な新規TGF-βシグナルの標的である (2009)
  • Author(s): 福西菜穂子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸コンベンションセンター(神戸)
  • Year and Date: 2009-10-23
• [Presentation] p63のΔNプロモータはケラチノサイト特異的な新規TGF-βシグナルの標的である (2009)
  • Author(s): 福西菜穂子, (ほか)
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2009-10-23
• [Presentation] p63 promotes proliferation of squamous cell carcinomas through a novel pathway of PP2A and GSK-3beta (2009)
  • Author(s): 福西菜穂子
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-10-02
• [Presentation] ΔNp63 is inducible by Smad2 and IKKα, the newly identified keratinocyte-specific TGF-μsignaling partners (2009)
  • Author(s): 加藤伊陽子
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-10-01
• [Presentation] DeltaN-p63 is inducible by Smad2 and IKKalpha, the newly identified keratinocyte-specific TGF-beta signaling partners (2009)
  • Author(s): Iyoko Katoh, (et al.)
  • Organizer: 第68回日本癌学会学術総会International Session
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2009-10-01
• [Remarks]
  • URL: http://sangaku.yamanashi.ac.jp/SearchResearcher/contents/7A93F75CD72F80F2.html
• [Remarks]
  • URL: http://sangaku.yamanashi.ac.jp/SearchResearcher/contents/7A93F75CD72F80F2.html
• [Remarks]
  • URL: http://sangaku.yamanashi.ac.jp/SearchResearcher/contents/7A93F75CD72F80F2.html
• [Remarks]
  • URL: http://sangaku.yamanashi.ac.jp/SearchResearcher/contents/7A93F75CD72F80F2.html