Studies on the mechanism to gain monocyte chemotactic capacity of ribosomal protein S19 and its role in coagulum resorption.

• Project/Area Number: 21590441
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kumamoto University
• Principal Investigator: YAMAMOTO Tetsuro 熊本大学, 大学院・生命科学研究部, 教授 (60112405)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: リボソームタンパク質S19 / プロトロンビン / 血液凝固反応 / 凝固XIII因子 / 血小板 / 線溶 / Glaドメイン / 血漿タンパク質 / ワーファリン / リボソームタンパク質 / 単球 / 白血球走化因子 / C5aレセプター / 凝血塊吸収 / S19リボソームタンパク質 / 単球走化因子 / 血液凝固XIII因子 / 細胞性線溶 / 血栓症

Research Abstract

In the present research, we have revealed that ribosomal protein S19(RP S19) present in normal blood plasma is covalently dimerized by activated factor XIII on the activated platelet surface and gains monocyte chemotactic capacity during blood coagulation process, that the RP S19 dimer recruits monocytes to the coagulum resulting in cellular fibrinolytic absorption of the coagulum, and that monocytotic state of peripheral blood enhance the coagulum resorption process.

Research Products

• [Journal Article] Ribosomal protein S19 is present in plasma as a complex with prothrombin (2011)
  • Author(s): Nishiura, H., Tanase, S., Tsujita, K., Sugiyama, S., Ogawa, ・H., Nakagaki, T., Semba, U., and Yamamoto, T.
  • Journal Title: Eur. J. Haematol. Volume: 86 Issue: 5 Pages: 436-441
  • DOI: 10.1111/j.1600-0609.2011.01585.x
  • Peer Reviewed
• [Journal Article] Role of Ribosomal Protein S19-Like Plasma Protein in Blood Coagulum Resorption. (2011)
  • Author(s): Ota, Y.
  • Journal Title: Exp.and Mol.Pathol. Volume: 90 Pages: 19-29
  • Peer Reviewed
• [Journal Article] Role of ribosomal protein S19-like plasma protein in blood coagulum resorption (2010)
  • Author(s): Ota, Y., Chen, J., Shin, M., Nishiura, H., Tokita, K., Shinohara, M., Yamamoto, T.
  • Journal Title: Exp. Mol. Pathol. Volume: 90 Pages: 19-28
  • Peer Reviewed
• [Journal Article] Base of molecular mimicry between human ribosomal protein S19 dimer and human C5a anaphylatoxin (2010)
  • Author(s): Nishiura, H., Chen, J., Ota, Y., Semba, U., Higuchi, H., Nakashima, T., Yamamoto, T.
  • Journal Title: Int. Immunopharmacol. Volume: 10(12) Pages: 1541-1547
  • NAID: 120003184562
  • Peer Reviewed
• [Journal Article] Inter-conversion between pure chemotactic ligands and chemoattractant/secretagogue ligands of neutrophil C5a receptor by a single amino acid substitution (2010)
  • Author(s): Jia, N., Semba, U., Nishiura, H., Kuniyasu, A., Nsiama, T. K., Nishino, N., and Yamamoto, T.
  • Journal Title: J. Leukocyt. Biol. Volume: 87 Pages: 965-975
  • Peer Reviewed
• [Journal Article] A Plasma Protein Indistinguishable from Ribosomal Protein S19 : Conversion to a Monocyte Chemotactic Factor by a Factor XIIIa-Catalyzed Reaction on Activated Platelet Membrane Phosphatidylserine in Association with Blood Coagulation (2010)
  • Author(s): Semba, U., Chen, J., Ota, Y., Jia, N., Arima, H., Nishiura, H., and Yamamoto, T.
  • Journal Title: Am. J. Pathol. Volume: 176 Pages: 542-1551
  • Peer Reviewed
• [Journal Article] Base of molecular mimicry between human ribosomal protein S19 dimer and human C5a anaphylatoxin. (2010)
  • Author(s): Nishiura, H.
  • Journal Title: Int.Immunopharmacol. Volume: 10(12) Pages: 1541-1547
  • Peer Reviewed
• [Journal Article] A Plasma Protein Indistinguishable from Ribosomal Protein S19 : Conversion to a Monocyte Chemotactic Factor by a Factor XIIIa-Catalyzed Reaction on Activated Platelet Membrane Phosphatidylserine in Association with Blond Coagulation (2010)
  • Author(s): U.Semba
  • Journal Title: Am.J.Pathol. 176 Pages: 1542-1551
  • Peer Reviewed
• [Journal Article] Inter-conversion between pure chemotactic ligands and chemoattractant/secretagogue ligands of neutrophil C5a receptor by a single amino acid substitution (2010)
  • Author(s): N.Jia
  • Journal Title: J.Leukocyt.Biol. (in press)
  • Peer Reviewed
• [Journal Article] Pro-and anti-apoptotic dual functions of the C5a receptor : Involvement of regulator of G protein signaling 3 and extracellular signal-regulated kinase (2009)
  • Author(s): Nishiura, H., Nonaka, H., Revollo, I., Semba, U., Li, Y., Irie, A., Harada, K., Kehrl, J. H. and Yamamoto, T.
  • Journal Title: Lab. Invest. Volume: 89 Pages: 676-694
  • Peer Reviewed
• [Journal Article] Pro- and anti-apoptotic dual functions of the C5a receptor : Involvement of regulator of G protein signaling 3 and extracellular signal-regulated kinase (2009)
  • Author(s): H.Nishiura
  • Journal Title: Lab.Invest. 89 Pages: 676-694
  • Peer Reviewed
• [Presentation] Ribosomal protein S19-prothrombin complex in plasma and its role in coagulum resorption (2011)
  • Author(s): Umeko Semba, Hiroshi Nishiura, Tetsuro Yamamoto
  • Organizer: 第36回日本微小循環学会総会
  • Place of Presentation: 愛知県・名古屋市立大学大ホール
  • Year and Date: 2011-02-11
• [Presentation] Ribosomal protein S19-prothrombin complex in plasma and its role in coagulum resorption. (2011)
  • Author(s): Semba, U.
  • Organizer: 第36回日本微小循環学会総会
  • Place of Presentation: 名古屋市立大学大ホール
  • Year and Date: 2011-02-11
• [Presentation] A molecular mimicry basis on the two-step binding structure to the C5a receptor between ribosomal protein S19 homodimer cross-linked by transglutaminase and C5a anaphylatoxin (2010)
  • Author(s): Hiroshi Nishiura, Tetsuro Yamamoto
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 兵庫県・神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Maintenance of ribosomal protein S19 in plasma by complex formation with prothrombin (2010)
  • Author(s): Umeko Semba, Hiroshi Nishiura, Sumio Tanase, Tetsuro Yamamoto
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 兵庫県・神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Maintenance of ribosomal protein S19 in plasma by complex formation with prothrombin. (2010)
  • Author(s): Semba, U.
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] A molecular mimicry basis on the two-step binding structure to the C5a receptor between ribosomal protein S19 homodimer cross-linked by transglutaminase and C5a anaphylatoxin. (2010)
  • Author(s): Nishiura, H.
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Conversion of a ribosomal protein S19-like plasma protein to a monocyte chemotactic factor on activated platelets during blood coagulation (2010)
  • Author(s): Tetsuro Yamamoto
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 兵庫県・神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] Conversion of a ribosomal protein S19-like plasma protein to a monocyte chemotactic factor on activated platelets during blood coagulation. (2010)
  • Author(s): Yamamoto, T.
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] Single amino acid conversions interchange between classical leukocyte chemoattractant and pure chemotactic factor (2010)
  • Author(s): 千場梅子
  • Organizer: 第35回日本微小循環学会総会
  • Place of Presentation: 大宮ソニックシティ
  • Year and Date: 2010-02-26
• [Presentation] 第2の補体C5aリセプターリガンドにより活性化するヒト肥満細胞株HMC-1内の新たなGi蛋白質依存性p38MAPK-TRPM経路 (2009)
  • Author(s): 西浦弘志, 山本哲郎
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県・神戸ポートアイランド
  • Year and Date: 2009-10-24
• [Presentation] 第2の補体C5aリセプターリガンドにより活性化するヒト肥満細胞株HMC-1内の新たなGi蛋白質依存性p38MAPK-TRPM経路 (2009)
  • Author(s): 西浦弘志
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-24
• [Presentation] S19リボソームタンパク質の凝固XIIIa因子による架橋二量体化とその凝血塊吸収における役割 (2009)
  • Author(s): 山本哲郎
  • Organizer: 第82回生化学会大会
  • Place of Presentation: 兵庫県・神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] S19リボソームタンパク質の凝固XIIIa因子による架橋二量体化とその凝血塊吸収における役割 (2009)
  • Author(s): 山本哲郎
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] 血漿に含まれるS19リボソームタンパク質様分子の血液凝固に伴う架橋二量体化機構 (2009)
  • Author(s): 千場梅子, 甚俊, 太田宜彦, 西浦弘志, 山本哲郎
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県・神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] 血漿に含まれるS19リボソームタンパク質様分子の血液凝固に伴う架橋二量体化機構 (2009)
  • Author(s): 千場梅子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-22
• [Presentation] 血漿のS19リボソームタンパク質様分子の二量体化と単球C5aレセプターの凝血塊吸収における役割 (2009)
  • Author(s): 太田宜彦, 千場梅子, 甚俊, 西浦弘志, 山本哲郎
  • Organizer: 第46回補体シンポジウム
  • Place of Presentation: 福岡県・九州大学西新プラザ大会議室
  • Year and Date: 2009-08-21
• [Presentation] 血漿のS19リボソームタンパク質様分子の二量体化と単球C5aレセプターの凝血塊吸収における役割 (2009)
  • Author(s): 太田宜彦
  • Organizer: 第46回補体シンポジウム
  • Place of Presentation: 九州大学 福岡
  • Year and Date: 2009-08-21
• [Presentation] 血漿中のS19リボソームタンパク質様分子の血液凝固に伴う単球走化活性の獲得と凝血塊除去における役割 (2009)
  • Author(s): 太田宜彦, 山本哲郎
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都府・国立京都国際会館
  • Year and Date: 2009-05-01
• [Presentation] 血漿中のS19リボソームタンパタ質様分子の血液凝固に伴う単球走化活性の獲得と凝血塊除去における役割 (2009)
  • Author(s): 太田宜彦
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立 京都国際会館
  • Year and Date: 2009-05-01
• [Book] Extraribosomal functions of ribosomal protein S19 In Ribosomal Proteins and Protein Engineering : Design, Selection and Applications(Valter Ortendhal, Hjalmar Salchow eds.) (2010)
  • Author(s): T. Yamamoto and H. Nishiura
  • Publisher: Nova Scientific Publishers
• [Book] Extraribosomal functions of ribosomal protein S19 In Ribosomal Proteins and Protein Engineering : Design, Selection and Applications. (Walter Ortendhal, Hjalmar Salchow eds.) (2010)
  • Author(s): T.Yamamoto
  • Publisher: Nova Scientific Publishers(in press)