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Study of Shiga toxin productions us ing in vivo analysis

Research Project

Project/Area Number 21590478
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionChiba University

Principal Investigator

SHIMIZU Takeshi  千葉大学, 大学院・医学研究院, 准教授 (70312840)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords腸管出血性大腸菌 / 志賀毒素 / 毒素産生 / 病原性 / NO / NO reductase / 感染症 / 細菌 / 分泌 / 細菌毒素 / 遺伝子発現
Research Abstract

A novel chromosome-plasmid hybrid bioluminescent reporter system (C-P reporter system) utilizing Photorhabdus luminescens luxCDABE genes has been constructed to monitor the expression of Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2) in enterohaemorrhagic Escherichia coli (EHEC) in real time. Using these reporter strains, we examined the profiles of Stx1 and Stx2 expression in EHEC. We found that production of both Stx1 and Stx2 in EHEC was enhanced upon contact with intestinal epithelial cells and within macrophages.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (32 results)

All 2012 2011 2010 2009 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (21 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Characterization of intracellular localization of PrP^<Sc> in prioninfected cells using monoclonal antibody that recognizes the region consisting of amino acids 119-127 of mouse PrP2012

    • Author(s)
      Yamasaki T., Suzuki A., Shimizu T., Watarai M., Hasebe R., Horiuchi M.
    • Journal Title

      Journal of General Virology

      Volume: vol.93 no.3 Issue: 3 Pages: 668-680

    • DOI

      10.1099/vir.0.037101-0

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recombinant Shiga toxin B subunit can induce neutralizing IgY antibody2012

    • Author(s)
      Paola Neri, Shunji Tokoro, Tsuyoshi Sugiyama, Kouji Umeda, Takeshi Shimizu, Takao Tsuji, Yoshikatsu Kodama, Hiroshi Mori
    • Journal Title

      Biol.Pharm.Bull.

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Specific egg yolk immunoglobulin as a new preventive approach for Shiga-toxin-mediated diseases2011

    • Author(s)
      Paola Neri, Shunji Tokoro, Ryo Kobayashi, Tsuyoshi Sugiyama, Kouji Umeda, Takeshi Shimizu, Takao Tsuji, Yoshikatsu Kodama, Keiji Oguma Hiroshi Mori
    • Journal Title

      PLoS ONE

      Volume: 6(10) Issue: 10 Pages: e26526-e26526

    • DOI

      10.1371/journal.pone.0026526

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a novel bioluminescent reportersystem for investigating Shiga toxin expression of enterohaemorrhagic2011

    • Author(s)
      Takeshi Shimizu, Yuko Ohta, Hiroyasu Tsutsuki Masatoshi Noda
    • Journal Title

      Escherichia coli. Gene

      Volume: 478 Issue: 1-2 Pages: 1-10

    • DOI

      10.1016/j.gene.2011.01.006

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a novel bioluminescent reporter system for investigating Shiga toxin expression of enterohaemorrhagic.Escbericbia coli2011

    • Author(s)
      Takeshi Shimizu
    • Journal Title

      Gene

      Volume: in press

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Shiga toxin 2 is specifically released from bacterial cells by two different mechanisms2009

    • Author(s)
      Takeshi Shimizu, Yuko Ohta Masatoshi Noda,
    • Journal Title

      Infect. Immun

      Volume: 77 Issue: 7 Pages: 2813-2823

    • DOI

      10.1128/iai.00060-09

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential entry of Botulinum neurotoxin A into neural andintestinal cells2009

    • Author(s)
      Aurelie Couesnon, Takeshi Shimizu Michel R. Popoff
    • Journal Title

      Cell. Microbiol

      Volume: 11 Issue: 2 Pages: 289-308

    • DOI

      10.1111/j.1462-5822.2008.01253.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Shiga toxin 2 is specifically released from bacterial cells by two different mechanisms.2009

    • Author(s)
      Takeshi Shimizu
    • Journal Title

      Infect.Immun. 77

      Pages: 2813-2823

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Yoshikatsu Kodama and Hiroshi Mori, 2012. Recombinant Shiga toxin B subunit can induce neutralizing IgY antibody

    • Author(s)
      Paola Neri, Shunji Tokoro, Tsuyoshi Sugiyama, Kouji Umeda, Takeshi Shimizu, Takao Tsuji
    • Journal Title

      Pharm. Bull

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] NO biosensor plasmid for investigating NO levels within macrophages during bacterial infection2012

    • Author(s)
      清水健、津々木博康、野田公俊
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] NO biosensor plasmid for investigating NO levels within macrophages during bacterial infection2012

    • Author(s)
      清水健, 津々木博康, 野田公俊
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎、長崎ブリックホール
    • Related Report
      2011 Final Research Report
  • [Presentation] The nitric oxide reductase of Enterohemorrahgic Escherichia coli playsan important role for the survival within macrophages2011

    • Author(s)
      清水健、津々木博康、野田公俊
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2011-09-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] 腸管出血性大腸菌の産生する志賀毒素について2011

    • Author(s)
      清水健
    • Organizer
      緊急セミナー:腸管出血性大腸菌の今
    • Place of Presentation
      東京、東京慈恵会医科大学(招待講演)
    • Year and Date
      2011-08-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 腸管出血性大腸菌NO reductaseは志賀毒素2の産生を増加させる2011

    • Author(s)
      清水健、津々木博康、野田公俊
    • Organizer
      第58回トキシンシンポジウム
    • Place of Presentation
      順天堂大学(東京)(招待講演)
    • Year and Date
      2011-07-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Haruaki Nakaya and Masatoshi Noda The nitric oxide reductase of enterohemorrhagic Escherichia coliO157:H7 increases Shiga toxin 2 production andplays an important role in its infection2011

    • Author(s)
      Takeshi Shimizu, Hiroyasu Tsutsuki, Akio Matsumoto
    • Organizer
      46^<th> Joint Conference on Cholera and Other Bacterial Enteric Infections Panel
    • Place of Presentation
      Kolkata, India
    • Related Report
      2011 Final Research Report
  • [Presentation] The nitric oxide reductase of enterohemorrahgic Escherichia coli plays an important role for the survival within macrophages2011

    • Author(s)
      Takeshi Shimizu, Hiroyasu Tsutsuki Masatoshi Noda
    • Organizer
      International Union of Microbiological Societies2011 Congress
    • Place of Presentation
      Sapporo Convention Center, Sapporo
    • Related Report
      2011 Final Research Report
  • [Presentation] 清水健腸管出血性大腸菌の産生する志賀毒素について2011

    • Author(s)
      清水健
    • Organizer
      緊急セミナー:腸管出血性大腸菌の今
    • Place of Presentation
      東京、東京慈恵会医科大学
    • Related Report
      2011 Final Research Report
  • [Presentation] 野田公俊腸管出血性大腸菌のNO reductaseは志賀毒素2の産生を増加させる2011

    • Author(s)
      清水健, 津々木博康
    • Organizer
      第58回トキシンシンポジウムシンポジウム指名講演
    • Place of Presentation
      東京、順天堂大学
    • Related Report
      2011 Final Research Report
  • [Presentation] The nitric oxide reductase of enterohemorrhagic Escherichia coli 0157 : H7 increases Shiga toxin 2 production and plays an important role in its infection2011

    • Author(s)
      Takeshi Shimizu, Hiroyasu Tsutsuki, Akio Matsumoto, Haruaki Nakaya, Masatoshi Noda
    • Organizer
      46^<th> US-Japan Cholera and Other Bacterial Enteric Infections Joint Panel Meeting
    • Place of Presentation
      インド(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Adherent effects of enterohemorrahgic Escberichia coli to human intestinal epithelial cells on its Shiga toxins production2010

    • Author(s)
      Takeshi Shimizu
    • Organizer
      45^<th> Joint Conference on Cholera and Other Bacterial Enteric Infections Panel
    • Place of Presentation
      Kyoto University, Kyoto
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 腸管出血性大腸菌の腸管上皮細胞への接着は病原因子の発現を増加させる2010

    • Author(s)
      清水健
    • Organizer
      第57回トキシンシンポジウム シンポジウム
    • Place of Presentation
      長浜、長浜ロイヤルホテル 指名講演
    • Year and Date
      2010-07-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Adherent effects of enterohemorrahgic Escherichia coli to human intestinal epithelial cells on its Shiga toxins production2010

    • Author(s)
      Takeshi Shimizu Masatoshi Noda
    • Organizer
      45^<th> Joint Conference on Cholera and OtherBacterial Enteric Infections Panel
    • Place of Presentation
      Kyoto University, Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] 腸管出血性大腸菌の腸管上皮細胞への接着は病原因子の発現を増加させる2010

    • Author(s)
      清水健, 野田公俊
    • Organizer
      第57回トキシンシンポジウムシンポジウム指名講演
    • Place of Presentation
      長浜、長浜ロイヤルホテル
    • Related Report
      2011 Final Research Report
  • [Presentation] 腸管上皮細胞に接着した腸管出血性大腸菌はその志賀毒素1の産生量を増加させる2010

    • Author(s)
      清水健, 野田公俊
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] 腸管出血性大腸菌が産生する志賀毒素の菌体外放出機構2009

    • Author(s)
      清水健
    • Organizer
      第92回日本細菌学会関東支部総会シンポジウム
    • Place of Presentation
      東京、東京医科歯科大学
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Effect of phage-inducing agent on the number of Stx2-expressed cells in Shiga toxin-producing Escherichia coli2009

    • Author(s)
      Takeshi Shimizu, Yuko Ohta Masatoshi Noda
    • Organizer
      44^<rd> Joint Conference on Cholera and OtherBacterial Enteric Infections Panel
    • Place of Presentation
      San Diego, California
    • Related Report
      2011 Final Research Report
  • [Presentation] 腸管出血性大腸菌が産生する志賀毒素2の菌体外放出機構の解析2009

    • Author(s)
      清水健, 野田公俊
    • Organizer
      第56回トキシンシンポジウムシンポジウム指名講演
    • Place of Presentation
      岐阜、アルモニーテラッセ
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] A mechanism of Stx2 release from enterohemorrahgic Escherichia coli2009

    • Author(s)
      Takeshi Shimizu, Yuko Ohta Masatoshi Noda
    • Organizer
      The 13^<th> International Conference on EmergingInfectious Diseases in the Pacific Rim
    • Place of Presentation
      Kolkata, India
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of phage-inducing agent on the number of Stx2-expressed cells in Shiga toxin-producing Escherichia coli.2009

    • Author(s)
      Takeshi Shimizu
    • Organizer
      44^<rd> Joint Conference on Cholera and Other Bacterial Enteric Infections Panel
    • Place of Presentation
      San Diego, California
    • Related Report
      2009 Annual Research Report
  • [Presentation] A mechanism of Stx2 release from enterohemorrahgic Escherichia coli2009

    • Author(s)
      Takeshi Shimizu
    • Organizer
      The 13^<th> International Conference on Emerging Infectious Diseases in the Pacific Rim
    • Place of Presentation
      Kolkata, India
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.m.chiba-u.ac.jp/class/molecular-infectiology/indexMI.htm

    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 一酸化窒素を探知するための形質転換用組換えベクター、およびこれを用いた一酸化窒素センサ細胞2011

    • Inventor(s)
      清水健, 津々木博康, 野田公俊
    • Industrial Property Rights Holder
      国立大学法人千葉大学
    • Industrial Property Number
      2011-101623
    • Filing Date
      2011-04-28
    • Related Report
      2011 Annual Research Report 2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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