Regulatory mechanisms of the strength of endotoxin signaling

• Project/Area Number: 21590502
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Musashino University
• Principal Investigator: TANAMOTO Kenichi 武蔵野大学, 薬学研究所, 教授 (60107430)
• Co-Investigator(Kenkyū-buntansha): MUROI Masashi 武蔵野大学, 薬学研究所, 准教授 (70311389)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: エンドトキシン / Toll様受容体 / マクロファージ / lipopolysaccharide / 敗血症 / 自然免疫 / Toll-like receptor / リピドA

Research Abstract

To clarify the regulatory mechanisms of the strength of endotoxin signaling, relationship between its activity and the binding to the receptor protein was studied. The affinity of lipid A compounds(active molecule of endotoxin) to human and mouse LBP was in following order ; 406>506>516. The order was roughly paralleled with their activities in murine cells but not with those in human cells, indicating that the process following the binding to LBP plays an important role in the regulation of the strength of endotoxin signaling.

Research Products

• [Journal Article] IRAK-1-mediated negative regulation of Toll-like receptor signaling through proteasome-dependent downregulation of TRAF6 (2012)
  • Author(s): Muroi M, Tanamoto K
  • Journal Title: Biochim. Biophys. Acta Volume: 1823 Pages: 255-263
  • Peer Reviewed
• [Journal Article] Novel anti-HIV-1 activity produced by conjugating unsulfated dextran with polyL-lysine (2012)
  • Author(s): Nakamura K, Ohtsuki T, Mori, Hoshino H, Hoque A, Oue A, Kano F, Sakagami H, Tanamoto K, Ushijima H, Kawasaki N, Akiyama H, Ogawa H
  • Journal Title: Antiviral Res Volume: 94 Pages: 89-97
  • Peer Reviewed
• [Journal Article] IRAK-1-mediated negative regulation of Toll-like receptor signaling through proteasome-dependent downregulation of TRAF6 (2012)
  • Author(s): Muroi M.
  • Journal Title: Biochim.Biophys.Acta Volume: 1823 Pages: 255-263
  • Peer Reviewed
• [Journal Article] Novel anti-HIV-1 activity produced by conjugating unsulfated dextran with polyL-lysine (2012)
  • Author(s): Nakamura K.
  • Journal Title: Antiviral Res. Volume: 94 Pages: 89-97
  • Peer Reviewed
• [Journal Article] Analysis of thickening polysaccharides by the improved diethyldithioacetal derivatization method (2011)
  • Author(s): Akiyama T, Yamazaki T, Tanamoto K
  • Journal Title: J. Food. Hyg. Soc. Japan Volume: 52 Pages: 40-6
  • NAID: 130000671810
  • Peer Reviewed
• [Journal Article] Application of matrix-assisted laser desorption ionization-time of flight mass spectrometry for discrimination of Escherichia strains possessing highly conserved ribosomal RNA gene sequences (2011)
  • Author(s): Muroi M., Shima K., Nakagawa Y., and Tanamoto K.
  • Journal Title: Biol. Pharm. Bull Volume: 34 Pages: 430-432
  • NAID: 130000657825
  • Peer Reviewed
• [Journal Article] IRAK-1によるTRAF6の分解を介したTLRシグナリングの抑制的調節 (2011)
  • Author(s): 室井正志
  • Journal Title: エンドトキシン・自然免疫研究 Volume: 14 Pages: 7-10
• [Journal Article] Application of matrix-assisted laser desorption ionization-time of flight mass spectrometry for discrimination of Escherichia strains possessing highly conserved ribosomal RNA gene sequences (2011)
  • Author(s): Muroi M.
  • Journal Title: Biol.Pharm.Bull. Volume: 34 Pages: 430-432
  • NAID: 130000657825
  • Peer Reviewed
• [Journal Article] Analysis of thickening polysaccharides by the improved diethyldithioacetal derivatization method (2011)
  • Author(s): Akiyama T.
  • Journal Title: J.Food.Hyg.Soc.Japan Volume: 52 Pages: 40-46
  • Peer Reviewed
• [Journal Article] Inhibitory effects of soluble MD-2 and soluble CD14 on bacterial growth (2010)
  • Author(s): Ohnishi T., Muroi M., Tanamoto K.
  • Journal Title: Microbiol. Immunol Volume: 54 Pages: 74-80
  • NAID: 10029543968
  • Peer Reviewed
• [Journal Article] Deoxynivalenol and nivalenol inhibit lipopolysaccharide-induced nitric oxide production by mouse macrophage cells (2010)
  • Author(s): Sugiyama K., Muroi M., Tanamoto K., Nishijima M., Sugita-Konishi Y.
  • Journal Title: Toxicol Lett Volume: 192 Pages: 150-4
  • Peer Reviewed
• [Journal Article] Urban aerosols induce pro-inflammatory cytokine production in macrophages and cause airway inflammation in vivo (2010)
  • Author(s): Yoshida, T., Yoshioka, Y., Fujimura, M., Kayamuro, H., Yamashita, K., Higashisaka, K., Nakanishi, R., Morishita, Y., Nabeshi, H., Yamashita, T., Muroi, M., Tanamoto, K., Nagano, K., Abe, Y., Kamada, H., Kawai, Y., Mayumi, T., Itoh, N., Yoshikawa, T., Tsun
  • Journal Title: Biol. Pharm. Bull Volume: 33 Pages: 780-783
  • NAID: 130000248036
  • Peer Reviewed
• [Journal Article] SDS-PAGEによる既存添加物酵素のたんぱく質分離パターン (2010)
  • Author(s): 秋山卓美、佐々木亮、山崎壮、棚元憲一、山形一雄、河村葉子
  • Journal Title: 日本食品化学学会誌 Volume: 17 Pages: 88-95
  • Peer Reviewed
• [Journal Article] Identification methods of terpenoid gum bases using TLC and GC/MS (2010)
  • Author(s): Akiyama T, Hayashi A, Yamazaki T, Tada A, Sugimoto N, Yun YS, Kunugi A, Tanamoto K, Kawamura Y
  • Journal Title: 食品衛生学雑誌 Volume: 51 Pages: 264-72
  • NAID: 130000454953
  • Peer Reviewed
• [Journal Article] 日本薬局方指定菌株の特性と保存管理法に関する研究III (2010)
  • Author(s): Tanamoto K., Muroi M., Igarashi M., Shima K., Nakagawa Y., Fujiwake H.
  • Journal Title: 医薬品医療機器レギュラトリーサイエンス Volume: 41 Pages: 890-894
• [Journal Article] Urban aerosols induce pro-inflammatory cytokine production in macrophages and cause airway inflammation in vivo (2010)
  • Author(s): Yoshida, T.
  • Journal Title: Biol.Pharm.Bull. Volume: 33 Pages: 780-783
  • Peer Reviewed
• [Journal Article] Identification methods of terpenoid gum bases using TLC and GC/MS (2010)
  • Author(s): Akiyama T.
  • Journal Title: 食品衛生学雑誌 Volume: 50 Pages: 264-272
  • Peer Reviewed
• [Journal Article] Deoxynivalenol and nivalenol inhibit lipopolisaccharide-induced nitric oxide production by mouse macrophage cells (2010)
  • Author(s): Sugiyama K
  • Journal Title: Toxicology Letters 192 Pages: 150-154
  • Peer Reviewed
• [Journal Article] Inhibitory effects of soluble MD-2 and soluble CD14 on bacterial growth and the role of lipopolysaccharide and peptidoglycan (2010)
  • Author(s): Ohnishi T
  • Journal Title: Microbiol Immunol 54 Pages: 74-80
  • Peer Reviewed
• [Journal Article] Urban aerosol induce pro-inflammatory cytokine production in macrophage and cause airway inflammation in vivo. (2010)
  • Author(s): Yoshida T
  • Journal Title: Biol Pharm Bull 33(5) Pages: 780-783
  • Peer Reviewed
• [Journal Article] Caspase-3 is activated and rapidly released from human umbilical vein endothelial cells in response to lipopolysaccharide (2009)
  • Author(s): Shioiri T., Muroi M., Hatao F., Nishida M., Ogawa T., Mimura Y., Seto Y., Kaminishi M., Tanamoto K.
  • Journal Title: Biochim. Biophys. Acta Volume: 1792 Pages: 1011-1018
  • Peer Reviewed
• [Journal Article] 日本薬局方指定菌株の特性と保存管理法に関する研究 (2009)
  • Author(s): Tanamoto K., Muroi M., Nakagawa Y., Shima K., Ichimura K.
  • Journal Title: Pharm. Regul. Sci Volume: 40 Pages: 520-524
• [Journal Article] Analysis of hexachlorobenzene in Food Red Nos. 104(phloxine) and 105(rose Bengal) by GC-ECD (2009)
  • Author(s): Kawasaki H, Furusho N, Tatebe C, Kubota H, Yanagi T, Yasukouchi Y, Mori Y, Yamashita Y, Iizuka T, Takahata K, Takahashi J, Sato K, Tanamoto K
  • Journal Title: J. Food. Hyg. Soc. Japan Volume: 50 Pages: 6-9
  • NAID: 130000671627
  • Peer Reviewed
• [Journal Article] Examination of original plant of Jamaica quassia extract, a natural bittering agent, based on composition of the constituents (2009)
  • Author(s): Tada A, Sugimoto N, Sato K, Akiyama T, Asanoma M, Yun YS, Yamazaki T, Tanamoto K
  • Journal Title: J. Food. Hyg. Soc. Japan Volume: 50 Pages: 16-21
  • NAID: 130000671629
  • Peer Reviewed
• [Journal Article] Migration tests of cadmium and lead from paint film of baby toys (2009)
  • Author(s): Kawamura Y, Mutsuga M, Yamauchi T, Ueda S, Tanamoto K
  • Journal Title: J. Food. Hyg. Soc. Japan Volume: 50 Pages: 93-96
  • NAID: 130000135374
  • Peer Reviewed
• [Journal Article] Cepaic acid, a novel xanthylium pigment from the dried outer scales of the yellow onion Allium cepa (2009)
  • Author(s): Ito Y., Sugimoto N., Akiyama T., Yamazaki T. & Tanamoto K
  • Journal Title: Tetrahedron Lett. Volume: 50 Pages: 4084-4086
  • Peer Reviewed
• [Journal Article] qNMRに基づく有機リン系農薬イソキサチオンオキソンの品質管理 (2009)
  • Author(s): 田原麻衣子、杉本直樹、末松孝子、有福和紀、斎藤剛、井原俊英、吉田雄一、多田敦子、久保田領志、清水久美子、山崎壮、棚元憲一、中澤裕之、西村哲治
  • Journal Title: 日本食品化学学会誌 Volume: 16 Pages: 28-33
  • NAID: 110007361212
  • Peer Reviewed
• [Journal Article] 既存添加物オゾケライトの成分調査 (2009)
  • Author(s): 多田敦子、杉本直樹、古庄紀子、石附京子、佐藤恭子、山崎壮、棚元憲一
  • Journal Title: 日本食品化学学会誌 Volume: 16 Pages: 92-96
  • NAID: 110007503391
  • Peer Reviewed
• [Journal Article] Analysis of residual solvent in thickeners by headspace gas chromatography using a standard addition method (2009)
  • Author(s): Tatebe C., Kawasaki H., Kubota H., Sato K., Tanamoto K. & Kawamura Y
  • Journal Title: Japn. J. Food Chem. Safety(JJFCS) Volume: 16 Pages: 78-83
  • NAID: 110007503389
  • Peer Reviewed
• [Journal Article] Analysis of primary aromatic amines in paper products (2009)
  • Author(s): Mutsuga M, Lee YK, Kawamura Y, Tanamoto K
  • Journal Title: Shokuhin Eiseigaku Zasshi Volume: 50 Pages: 160-166
  • NAID: 130000135384
  • Peer Reviewed
• [Journal Article] Caspase-3 is activated and rapidly released from human umbilical vein endothelial cells in response to lipopolysaccharide. (2009)
  • Author(s): Shiroi T
  • Journal Title: Biochim Biophys Acta 1792 Pages: 1011-1018
  • Peer Reviewed
• [Journal Article] Cepaic acid, a novel xanthylium pigment from the dried outer scales of the yellow onion Allium cepa (2009)
  • Author(s): Ito Y
  • Journal Title: Tetrahedron Letters 50 Pages: 4084-4086
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 棚元憲一
  • Journal Title: 「日本薬局方の試験法に関する研究」日本薬局法試験菌株の特性と保存管理に関する研究(財団法人 日本公定書協会) Pages: 520-524
• [Journal Article] (2009)
  • Author(s): 室井正志
  • Journal Title: エンドトキシン研究11(医学図書出版) Pages: 17-19
• [Journal Article] (2009)
  • Author(s): 杉山圭一
  • Journal Title: エンドトキシン研究11(医学図書出版) Pages: 20-22
• [Journal Article] (2009)
  • Author(s): 室井正志
  • Journal Title: エンドトキシン研究12(医学図書出版) Pages: 69-71
• [Journal Article] (2009)
  • Author(s): 大西貴弘
  • Journal Title: エンドトキシン研究12(医学図書出版) Pages: 72-74
• [Journal Article] (2009)
  • Author(s): 杉山圭一
  • Journal Title: エンドトキシン研究12(医学図書出版) Pages: 81-83
• [Presentation] ミクログリア細胞におけるToll-like receptorの発現及び炎症性応答 (2012)
  • Author(s): 林原紀明、田村直樹、渡辺恵、室井正志、畑尾史彦, 瀬戸泰之, 棚元憲一
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎、長崎ブリックホール
  • Year and Date: 2012-03-29
• [Presentation] ミクログリア細胞におけるToll-like receptorの発現及び炎症性応答 (2012)
  • Author(s): 林原紀明
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎)
  • Year and Date: 2012-03-29
• [Presentation] ダニアレルゲンの自然免疫応答に及ぼす影響 (2012)
  • Author(s): 北島孝明, 室井正志, 山下直美, 棚元憲一
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎、長崎ブリックホール
  • Year and Date: 2012-03-27
• [Presentation] ダニアレルゲンの自然免疫応答に及ぼす影響 (2012)
  • Author(s): 北島孝明
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎)
  • Year and Date: 2012-03-27
• [Presentation] Trichothecene mycotoxins inhibit MyD88-independent pathways of Toll-like receptors (2011)
  • Author(s): Sugiyama, K., Kinoshita, M., Minai, Y., Muroi, M., Tanamoto, K. and Sugita-Konishi, Y
  • Organizer: 9th Joint Meeting of ICS-ISICR
  • Place of Presentation: イタリア、Monash University Prato Centre
  • Year and Date: 2011-10-10
• [Presentation] Trichothecene mycotoxins inhibit MyD88-independent pathways of Toll-like receptors (2011)
  • Author(s): Sugiyama K.
  • Organizer: 9th Joint Meeting of ICS-ISICR
  • Place of Presentation: Palazzo dei Congressi (Italy)
  • Year and Date: 2011-10-10
• [Presentation] IRAK-1-mediated negative regulation of Toll-like receptor signaling (2011)
  • Author(s): Masashi Muroi and Ken-ichi Tanamoto
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌、札幌コンベンションセンター
  • Year and Date: 2011-09-08
• [Presentation] IRAK-1-mediated negative regulation of Toll-like receptor signaling (2011)
  • Author(s): Muroi M.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-08
• [Presentation] 光散乱エンドトキシン測定法分析法バリデーション (2011)
  • Author(s): 杉浦友香、廣野泰亮、室井正志、棚元憲一
  • Organizer: 日本防菌防黴学会第38回年次大会
  • Place of Presentation: 大阪、千里ライフサイエンスセンター
  • Year and Date: 2011-08-31
• [Presentation] マトリックス支援レーザー脱離イオン化質量分析法による遺伝的近縁菌株の識別解析 (2011)
  • Author(s): 室井正志、島圭介、中川恭好、棚元憲一
  • Organizer: 日本防菌防黴学会第38回年次大会
  • Place of Presentation: 大阪、千里ライフサイエンスセンター
  • Year and Date: 2011-08-31
• [Presentation] 光散乱エンドトキシン測定法分析法バリデーション (2011)
  • Author(s): 杉浦友香
  • Organizer: 日本防菌防黴学会第38回年次大会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2011-08-31
• [Presentation] マトリックス支援レーザー脱離イオン化質量分析法による遺伝的近縁菌株の識別解析 (2011)
  • Author(s): 室井正志
  • Organizer: 日本防菌防黴学会第38回年次大会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2011-08-31
• [Presentation] マトリックス支援レーザー脱離イオン化質量分析法による遺伝的近縁菌株の識別解析 (2011)
  • Author(s): 室井正志、島圭介、中川恭好、棚元憲一
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡、ツインメッセ静岡
  • Year and Date: 2011-03-30
• [Presentation] 光散乱エンドトキシン測定法における攪拌回転速度の検討 (2011)
  • Author(s): 杉浦友香、廣野泰亮、室井正志、棚元憲一
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡、グランシップ
  • Year and Date: 2011-03-30
• [Presentation] マトリックス支援レーザー脱離イオン化質量分析法による遺伝的近縁菌株の識別解析 (2011)
  • Author(s): 室井正志
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: ツインメッセ静岡(静岡県)
  • Year and Date: 2011-03-30
• [Presentation] 光散乱エンドトキシン測定法における攪拌回転速度の検討 (2011)
  • Author(s): 杉浦友香
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: ツインメッセ静岡(静岡県)
  • Year and Date: 2011-03-30
• [Presentation] IRAK-1によるTRAF6の分解を介したTLRシグナリングの抑制的調節 (2010)
  • Author(s): 室井正志、棚元憲一
  • Organizer: 第16回日本エンドトキシン・自然免疫研究会
  • Place of Presentation: 奈良、かしはら万葉ホール
  • Year and Date: 2010-11-13
• [Presentation] IRAK-1によるTRAF6の分解を介したTLRシグナリングの抑制的調節 (2010)
  • Author(s): 室井正志
  • Organizer: 第16回日本エンドトキシン・自然免疫研究会
  • Place of Presentation: かしはら万葉ホール(奈良県)
  • Year and Date: 2010-11-13
• [Presentation] Stimulation of Toll-like receptors leads to proteasome-dependent downregulation of TRAF6 through interaction with IRAK-1 (2010)
  • Author(s): Masashi Muroi and Ken-ichi Tanamoto
  • Organizer: Annual Meeting of The Society for Leukocyte Biology & The International Endotoxin and Innate Immunity Society
  • Place of Presentation: バンクーバー、Fairmont Hotel
  • Year and Date: 2010-10-09
• [Presentation] Stimulation of Toll-like receptors leads to proteasome-dependent downre gulation of TRAF6 through interaction with IRAK-1 (2010)
  • Author(s): Muroi M.
  • Organizer: Annual Meeting of The Society for Leukocyte Biology & The International Endotoxin and Innate Immunity Society
  • Place of Presentation: Vancouver(Canada)
  • Year and Date: 2010-10-09
• [Presentation] IRAK-1により誘導されるTRAF6のproteasome依存性の分解 (2010)
  • Author(s): 室井正志、棚元憲一
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山、桃太郎アリーナ
  • Year and Date: 2010-03-30
• [Presentation] TRAF6はIRAK-1と相互作用することによりproteasome依存性に分解される (2010)
  • Author(s): 室井正志、棚元憲一
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜、パシフィコ横浜
  • Year and Date: 2010-03-29
• [Presentation] TRAF6はIRAK-1と相互作用することによりproteasome依存性に分解される (2010)
  • Author(s): 室井正志
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(神奈川県)
• [Presentation] IRAK-1により誘導されるTRAF6のproteasome依存性の分解 (2010)
  • Author(s): 室井正志
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山県桃太郎アリーナ(岡山県)
• [Presentation] TLRシグナルに対するdeoxynivalenolの抑制機構 (2009)
  • Author(s): 杉山圭一、室井正志、棚元憲一、小西良子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸、神戸国際展示場
  • Year and Date: 2009-10-24
• [Presentation] 非病原性細菌による感染症に対する内分泌かく乱候補物質の影響評価 (2009)
  • Author(s): 水谷紀子、室井正志、五十嵐ありさ、菅野慎二、鎌田洋一、小西良子、棚元憲一
  • Organizer: 第16回日本免疫毒性学会学術大会
  • Place of Presentation: 旭川、市民文化会館
  • Year and Date: 2009-08-07
• [Presentation] 非結晶性ナノシリカの自然免疫抑制作用に関する検討 (2009)
  • Author(s): 森重智弘、吉岡靖雄、田辺綾、堤康央、室井正志、棚元憲一、河合裕一、眞弓忠範、向洋平、岡田直貴、中川晋作
  • Organizer: 第36回日本トキシコロジー学会
  • Place of Presentation: 盛岡、アイーナ
  • Year and Date: 2009-07-17
• [Presentation] 非結晶性ナノシリカの自然免疫抑制作用に関する検討 (2009)
  • Author(s): 森重智弘
  • Organizer: 第36回日本トキシコロジー学会
  • Place of Presentation: 岩手県民情報交流センター(岩手県)
• [Presentation] 非病原性細菌による感染症に対する内分泌かく乱候補物質の影響評価 (2009)
  • Author(s): 水谷紀子
  • Organizer: 第16回日本免疫毒性学会学術大会
  • Place of Presentation: 旭川市民文化会館(北海道
• [Presentation] TLRシグナルに対するdeoxynivalenolの抑制機構 (2009)
  • Author(s): 杉山圭一
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Book] IRAK-1によるTRAF6の分解を介したTLRシグナリングの抑制的調節、エンドトキシン・自然免疫研究 (2011)
  • Author(s): 室井正志、棚元憲一
  • Publisher: 医学図書出版