Identification and functional analysis of anti-HIV host factor in macrophages

• Project/Area Number: 21590511
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Kumamoto University
• Principal Investigator: FUJITA Mikako 熊本大学, 薬学部, 准教授 (00322256)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Vpx / HIV-2 / アクセサリー蛋白質 / マクロファージ / 逆転写反応 / 抗HIV宿主因子 / PMA / THP-1

Research Abstract

Recently, it has been reported that there are an anti-HIV host factors which inhibit reverse transcription of the viral genome macrophages-specifically, as a target factors of HIV-2 Vpx protein. In this study, preparation for identification of these host factors had been performed. However, in 2011, SAMHD1and APOBEC3A were identified as these factors, and almost all functional details of these proteins have been revealed. Thus, in this study, roles of C-terminal polyproline motif(PPM) have been examined. As a result, we suggested that PPM suppresses lysosomal degradation and proteasome degradation of Vpx protein.

Research Products

• [Journal Article] Structural biology for virus research (2012)
  • Author(s): Yasuyuki Miyazaki, Mikako Fujita, Masako Nomaguchi, Akio Adcahi
  • Journal Title: Frontiers in Microbiology Volume: 3 Pages: 91-91
  • Peer Reviewed
• [Journal Article] Commentary on aptamers for virus research (2012)
  • Author(s): Yasuyuki Miyazaki, Mikako Fujita
  • Journal Title: Frontiers in Microbiology Volume: 3 Pages: 52-52
  • Peer Reviewed
• [Journal Article] An artificial copper complex incorporating a cell-penetrating peptide inhibits NF-・B activation (2011)
  • Author(s): Yosuke Kanemaru, Yumi Momiki, Saori Matsuura, Tatsufumi Horikawa, Jin Gohda, Jun-ichiro Inoue, Yoshinari Okamoto, Mikako Fujita, Masami Otuska
  • Journal Title: Chemical & Pharmaceutical Bulletin Volume: 59 Pages: 1555-1558
  • Peer Reviewed
• [Journal Article] An anti-HIV-1compound that increases steady-state expression of apoplipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (2011)
  • Author(s): Tomohiko Ejima, Mayuko Hirota, Tamio Mizukami, Masami Otsuka, Mikako Fujita
  • Journal Title: International Journal of Molecular Medicine Volume: 28 Pages: 613-616
  • Peer Reviewed
• [Journal Article] HIV-1 Nef impairs multiple T-cell functions in antigen-specific immune response in mice (2011)
  • Author(s): Hideki Fujii, Manabu Ato, Yoshimasa Takahashi, Kaori Otake, Shu-ichi Hashimoto, Tomohiro Kaji, Yasuko Tsunetsugu-Yokota, Mikako Fujita, Akio Adachi, Toshinori Nakamura, Masaru Taniguchi, Shigeo Koyasu, Toshiitada Takemori
  • Journal Title: International Immunology Volume: 23 Pages: 433-441
  • Peer Reviewed
• [Journal Article] The fourth major restriction factor against HIV/SIV (2011)
  • Author(s): Masako Nomaguchi, Mikako Fujita, Akio Adachi
  • Journal Title: Frontiers in Microbiology Volume: 2 Pages: 132-132
  • Peer Reviewed
• [Journal Article] The fourth major restriction factor against HIV/ SIV (2011)
  • Author(s): Nomaguchi, M., Fujita, M., and Adachi, A
  • Journal Title: Frontiers in Microbiology Volume: 2 Pages: 132-132
  • DOI: 10.3389/fmicb.2011.00132
  • Peer Reviewed
• [Journal Article] Site-directed mutagenesis of HIV-1 vpu gene demonstrates two clusters of replication-defective mutants with distinct ability to down-regulate cell surface CD4 and tetherin (2010)
  • Author(s): Masako Nomaguchi, Naoya Doi, Sachi Fujiwara, Mikako Fujita, Akio Adachi
  • Journal Title: Frontiers in Microbiology Volume: 1 Pages: 116-116
  • Peer Reviewed
• [Journal Article] Highly sensitive analysis of the interaction between HIV-1Gag and phosphoinositide derivatives based on surface plasmon resonance (2010)
  • Author(s): Kensaku Anraku, Ryota Fukuda, Nobutoki Takamune, Shogo Misumi, Yoshinari Okamoto, Masami Otsuka, Mikako Fujita
  • Journal Title: Biochemistry Volume: 49 Pages: 5109-5116
  • Peer Reviewed
• [Journal Article] Multifaced activity of HIV Vpr/Vpx proteins : the current view of their virological functions (2010)
  • Author(s): Mikako Fujita, Masami Otsuka, Masako Nomaguchi, Akio Adachi
  • Journal Title: Reviews in Medical Virology Volume: 20 Pages: 68-76
  • Peer Reviewed
• [Journal Article] Role of HIV-1Nef protein for virus replication in vitro (2010)
  • Author(s): Abhay Jere, Mikako Fujita, Akio Adachi, Masako Nomaguchi
  • Journal Title: Microbes and Infection Volume: 12 Pages: 65-70
  • Peer Reviewed
• [Presentation] 抗エイズ活性をもつイノシトールリン脂質誘導体の合成 (2012)
  • Author(s): 安楽健作、立石大、藤田美歌子、大塚雅巳
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-03-31
• [Presentation] 酸性脂質類によるHIV-1増殖の抑制 (2011)
  • Author(s): 安楽健作、大塚雅巳、藤田美歌子
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: ハイアットリージェンシー東京(東京)
  • Year and Date: 2011-12-02
• [Presentation] PHドメイン分子によるHIV-1放出の抑制 (2011)
  • Author(s): 藤田美歌子、安楽健作、大塚雅巳
  • Organizer: 第25回日本エイズ学会学術集会
  • Place of Presentation: ハイアットリージェンシー東京(東京)
  • Year and Date: 2011-11-30
• [Presentation] HIV-1増殖を制御するフラーレン化合物の開発 (2010)
  • Author(s): 藤田美歌子、大塚雅巳、高橋恭子、中村成夫、増野匡彦
  • Organizer: 第24回日本エイズ学会学術集会
  • Place of Presentation: グランドプリンスホテル高輪/ザ・プリンスさくらタワー東京(東京)
  • Year and Date: 2010-11-25
• [Presentation] HIV MAおよびNef蛋白質と脂質類との結合解析 (2010)
  • Author(s): 福田亮太、安楽健作、大塚雅巳、藤田美歌子
  • Organizer: 第24回日本エイズ学会学術集会
  • Place of Presentation: グランドプリンスホテル高輪/ザ・プリンスさくらタワー東京(東京)
  • Year and Date: 2010-11-24
• [Presentation] HIV Vpr/Vpx蛋白質の役割 (2010)
  • Author(s): 藤田美歌子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-08
• [Presentation] HIV-1Gag関連蛋白質およびNefとイノシトールリン酸類との結合解析 (2009)
  • Author(s): 福田亮太、安楽健作、大塚雅巳、藤田美歌子
  • Organizer: 第23回日本エイズ学会学術集会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2009-11-26
• [Presentation] HIV-1複製に関与するイノシトールリン脂質類とGag関連蛋白質との結合の定量的解析 (2009)
  • Author(s): 藤田美歌子、安楽健作、大塚雅巳
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京)
  • Year and Date: 2009-10-26
• [Patent(Industrial Property Rights)] APOBEC発現向上剤及び抗HIV剤 (2010)
  • Inventor(s): 藤田美歌子、大塚雅巳、江島智彦
  • Industrial Property Rights Holder: 熊本大学
  • Industrial Property Number: 2010-103182
  • Filing Date: 2010-04-28