Novel Marker of oxidative stress by new radical trap agent

• Project/Area Number: 21590633
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kyushu University of Health and Welfare
• Principal Investigator: SATO Keizo 九州保健福祉大学, 薬学部, 教授 (00315293)
• Co-Investigator(Kenkyū-buntansha): 中川 和子 熊本大学, 大学院・医学薬学研究部, 教授 (20284747)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 臨床 / 医療・福祉 / 薬学 / 感染症

Research Abstract

We found that Novel Marker of oxidative stress by new radical trap agent using our developed new spin trap agent, 5-diphenylphosphoryl-5-methyl-pyrroline-N-Oxide(DPPMPO). It is very useful for free radical detection in not only chemical, cellular, enzymatical system but also in vivo human system by using ESR spin trap extraction method with DPPMPO.

Research Products

• [Journal Article] Effects of lecithinized superoxide dismutase and/or pirfenidone against bleomycin-induced pulmonary fibrosis (2012)
  • Author(s): Tanaka K, Azuma A, Miyazaki Y, Sato K, Mizuhisma T
  • Journal Title: Chest Volume: (in press)
  • Peer Reviewed
• [Journal Article] In vivo evidence of free radical generation in the mouse lung after exposure to Pseudomonas aeruginosa bacterium : an ESR spin-trapping investigation (2012)
  • Author(s): Sato K, Corbett J, Mason RP, Kadiiska MB
  • Journal Title: Free Radic Res Volume: 46巻 Pages: 645-55
  • Peer Reviewed
• [Journal Article] Phosphoenoipyruvic Acid, an Intermediary Metabolite of Glycolysis, as a Potential Cytoprotectant and Anti-oxidant in Hela Cells (2012)
  • Author(s): Kondo Y, Ishithuka Y, Kadowaki D, Kuroda M, Tanaka Y, Nagatomi M, Irikura M, Hirata S, Sato K, Maruyama T, Hamasaki N, and Irie T
  • Journal Title: Biol. Pharm. Bull Volume: 35巻 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Phosphoenoipyruvic Acid, an Intermediary Metabolite of Glycolysis, as a Potential Cytoprotectant and Anti-oxidant in Hela Cells (2012)
  • Author(s): Kondo Y, Ishithuka Y, Kadowaki D, Kuroda M, Tanaka Y, Nagatomi M, Irikura M, Hirata S, Sato K., Maruyama T, Hamasaki N, Irie T
  • Journal Title: Biol.Pharm.Bull Volume: 35 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Therapeutic effect of lecithinized superoxide dismutase on pulmonary emphysema (2011)
  • Author(s): Tanaka K, Tanaka Y, Miyazaki Y, Namba T, Sato K, Aoshiba K, Azuma A, Mizushima T
  • Journal Title: J PharmacolExpTher Volume: 338巻 Pages: 810-818
  • Peer Reviewed
• [Journal Article] Therapeutic effect of lecithinized superoxide dismutase on pulmonary emphysema (2011)
  • Author(s): Tanaka K, Tanaka Y, Miyazaki Y, Namba T, Sato K, Aoshiba K, Azuma A, Mizushima T
  • Journal Title: J Pharmacol Exp Ther Volume: 338 Pages: 810-818
  • Peer Reviewed
• [Journal Article] マクロライド薬作用の新知見 (2011)
  • Author(s): 新屋智寛, 佐藤圭創
  • Journal Title: 感染症 Volume: 41 Pages: 1-9
• [Journal Article] Curcumin decreases toll-like receptor-2 gene expression and function in human monocytes and neutrophils. (2010)
  • Author(s): Shuto T.
  • Journal Title: Biochem Biophys Res Commun. Volume: 398 Pages: 647-652
  • Peer Reviewed
• [Journal Article] The uremic solute indoxyl sulfate acts as an antioxidant against superoxide anion radicals under normal-physiological conditions. (2010)
  • Author(s): Miyamoto Y.
  • Journal Title: FEBS Lett Volume: 584 Pages: 2816-2820
  • Peer Reviewed
• [Journal Article] Therapeutic effect of lecithinized superoxide dismutase on bleomycin-induced pulmonary fibrosis. (2010)
  • Author(s): Tanaka K.
  • Journal Title: Am J Physiol Lung Cell Mol Physiol. Volume: 298
  • Peer Reviewed
• [Journal Article] Prevention of ultraviolet B radiation-induced epidermal damage by expression of heat shock protein 70. (2010)
  • Author(s): Matsuda, M.
  • Journal Title: J.Biol.Chem. 285 Pages: 5848-5858
  • Peer Reviewed
• [Journal Article] Positive role of CCAAT/enhancer-binding protein homologous protein, a transcription factor involved in the endoplasmic reticulum stress response in the development of colitis. (2009)
  • Author(s): Namba, T.
  • Journal Title: Am.J.Pathol. 174 Pages: 1786-1798
  • Peer Reviewed
• [Presentation] リウマチ性疾患と肺病変 (2011)
  • Author(s): 佐藤圭創
  • Organizer: 福岡地区リウマチ教育研修会
  • Place of Presentation: 福岡
  • Year and Date: 2011-12-11
• [Presentation] リウマチ性疾患と肺病変 (2011)
  • Author(s): 佐藤圭創
  • Organizer: 福岡地区リウマチ教育研修会
  • Place of Presentation: 福岡(特別講演)
  • Year and Date: 2011-12-11
• [Presentation] 感染症の現在・過去・未来-病態を考えた未来の感染症治療を目指して- (2011)
  • Author(s): 佐藤圭創
  • Organizer: 第21回日本耳科学会総会・学術講演会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-11-24
• [Presentation] 感染症の現在・過去・未来-病態を考えた未来の感染症治療を目指して- (2011)
  • Author(s): 佐藤圭創
  • Organizer: 第21回日本耳科学会総会・学術講演会
  • Place of Presentation: 沖縄(特別講演)
  • Year and Date: 2011-11-24
• [Presentation] マクロライドの新作用としての気道炎症抑制作用 (2011)
  • Author(s): 佐藤圭創
  • Organizer: 生体機能と創薬シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-09-01
• [Presentation] マクロライドの新作用としての気道炎症抑制作用 (2011)
  • Author(s): 佐藤圭創
  • Organizer: 生体機能と創薬シンポジウム
  • Place of Presentation: 東京(特別講演)
  • Year and Date: 2011-09-01
• [Presentation] 呼吸器感染における炎症抑制の最前線 (2011)
  • Author(s): 佐藤圭創
  • Organizer: 第59回日本化学療法学会総会イブニングセミナー4
  • Place of Presentation: 札幌
  • Year and Date: 2011-06-24
• [Presentation] インフルエンザの劇症化機序とマクロライドの役割 (2011)
  • Author(s): 佐藤圭創
  • Organizer: 第73回耳鼻咽喉科臨床学会総会・学術講演会
  • Place of Presentation: 長野
  • Year and Date: 2011-06-24
• [Presentation] 呼吸器感染における炎症抑制の最前線 (2011)
  • Author(s): 佐藤圭創
  • Organizer: 第59回日本化学療法学会総会イブニングセミナー4
  • Place of Presentation: 札幌(特別講演)
  • Year and Date: 2011-06-24
• [Presentation] インフルエンザの劇症化機序とマクロライドの役割 (2011)
  • Author(s): 佐藤圭創
  • Organizer: 第73回耳鼻咽喉科臨床学会総会・学術講演会
  • Place of Presentation: 長野(特別講演)
  • Year and Date: 2011-06-24
• [Presentation] Low- dose and long- term therapy of clarithromycin could regulate the inflammation in human influenza virus infection. (2010)
  • Author(s): Sato K.
  • Organizer: 2010年米国呼吸器学会
  • Place of Presentation: ニューオリンズ、米国
  • Year and Date: 2010-05-17
• [Presentation] Low- dose and long- term therapy of clarithromycin could regulate the inflammation in human influenza virus infection. (2009)
  • Author(s): 佐藤圭創
  • Organizer: 47th Annual Meeting of the Infectious Diseases Society of America
  • Place of Presentation: フィラデルフィアコンベンションセンター(米国)