Identifications of tentative pancreatic cancer-related antigens of expressed proteins derived from membrane and nuclei components of 4 pancreatic cancer cell lines by autoantibodiomics

• Project/Area Number: 21590642
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Osaka Medical College
• Principal Investigator: MIYAMOTO Yoshiharu 大阪医科大学, 医学部, 助教 (20368096)
• Co-Investigator(Kenkyū-buntansha): NAKANISHI Toyofumi 大阪医科大学, 医学部, 准教授 (10247843) ; MATSUMURA Yoko 大阪医科大学, 医学部, 助教 (80388256) ; TAKEUCHI Touru 大阪医科大学, 医学部, 助教 (10330078) ; TAKUBO Takayuki 大阪医科大学, 医学部, 教授 (60163359) ; TAKAORI Kyoichi 京都大学, 医学部, 講師 (10329485)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵臓癌 / 自己抗体 / プロテオミクス / 細胞膜/核内タンパク質 / Autoantibodiomics / Pancreatic cancer / Triosephosphate isomerase / Candidate marker / 細胞膜 / 核内タンパク質 / 膵臓癌特異抗原

Research Abstract

Pancreatic cancer has one of the poor prognoses of all neoplasm, with a 5-year survival rate of below 10%, accounting fifth largest number of cancer deaths. In pancreatic cancer, autoimmunity has been also identified against several proteins but it is not clear why only patients infiltrated with a particular tumor type develop a humoral response to cancer-associated antigens. At this time, we applied the autoantibodiomics to identify pancreatic cancer-related antigens and antibodies, and examined 33 sera samples of patients with pancreatic cancer to identify the autoantibodies against expressed cytosolic, nuclear and membrane proteins derived from four typical human pancreatic cancer cell lines. We identified 6 candidate biomarkers, such as MAP7 domain conaining 2, carbonic anhydrase 1, ubiquilin-like protein, pyruvate kinase M1/M2, FAST kinase domain containg protein2 and pericentriolar material 1 to detect the pancreatic cancer. The antibodies against to these 6 proteins were highly detected in plasma of the cancer patients. We should develop the ELISA system to detect these 6 candidate biomarkers for the screening of the pancreatic cancer.

Research Products

• [Journal Article] Imaging of methamphetamine incorporated into hair using matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (2011)
  • Author(s): Miki A, Katagi M, Shima N, Kamata T, Tatsuno M, Nakanishi T, Tsuchihashi H, Takubo T, Suzuki K.
  • Journal Title: Forensic Tox Volume: 29 Pages: 111-6
• [Journal Article] MALDI-TOF and MALDI-FTICR imaging mass spectrometry of methamphetamine incorporated into hair (2011)
  • Author(s): Miki A, Katagi M, Kamata T, Zaitsu K, Tatsuno M, Nakanishi T, Tsuchihashi H, Takubo T, Suzuki K.
  • Journal Title: J. Mass Spectrom. Volume: 46(4) Pages: 411-6
• [Journal Article] S-sulfonation of transthyretin is an important trigger step in the formation of transthyretin-related amyloid fibril (2010)
  • Author(s): Nakanishi T, Yoshioka M, Moriuchi K, Yamamoto D, Tsuji M, Takubo T.
  • Journal Title: Biochim. Biophys Acta Volume: 1804(4) Pages: 1449-56
• [Journal Article] Proteomics-based identification of a tumor associated antigen and its corresponding autoantibody in gastric cancer (2010)
  • Author(s): Tunemi S, Nakanishi T, Fujita Y, Bouras G, Miyamoto Y, Miyamoto A, Sakai A, Noumra E, Takubo T, Tanigawa N.
  • Journal Title: Oncology Reports Volume: 23 Pages: 949-56
• [Presentation] The detection of disease-specific proteins in clinical samples by the soft-ionization mass spectrometry (2011)
  • Author(s): Nakanishi, T
  • Organizer: IUPAC international Congress on Analytical Sciences 2011
  • Place of Presentation: Kyoto
• [Presentation] The detection of disease-specific proteins in clinical samples by the soft-ionization mass spectrometry (2011)
  • Author(s): Nakanishi, T
  • Organizer: IUPAC international Congress on Analytical Sciences 2011
  • Place of Presentation: 国立京都国際会館
• [Presentation] Detetction of alpha-enolase and triosephosphate isomerase in sera from patients with pancreatic cancer by autoantibodiomics analysis (2010)
  • Author(s): Nakanishi T, Takaori K, C. Iacobuzio-Donahue A, Yachida S, Hruban RH, Miyamoto Y, Tanigawa N, Tabushi Y, Takubo T.
  • Organizer: 58^<th> ASMS Conference on Mass Spectrometry
  • Place of Presentation: Salt Lake, CO, USA
• [Presentation] Detetction of alpha-enolase and triosephosphate isomerase in sera from patients with pancreatic cancer by autoantibodiomics analysis. (2010)
  • Author(s): Nakanishi T, Takaori K, C.Iacobuzio-Donahue A, Yachida S, Hruban RH, Miyamoto Y, Tanigawa N, Y.Y.Tabushi Y, Takubo T.
  • Organizer: 58^<th> ASMS Conference on Mass Spectrometry
  • Place of Presentation: Salt Lake, CO, USA
• [Book] 『臨床プロテオーム』個別化医療を目指す臨床バイオマーカー探索 (2012)
  • Author(s): 加藤治文、中村和行、西尾和人、西村俊秀
  • Publisher: 金原出版