| Project/Area Number |
21590669
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
|
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Yukiyo 日本医科大学, 医学部, 助教 (40322515)
KAWADA Tomoyuki 日本医科大学, 医学(系)研究科(研究院), 教授 (00224791)
OTSUKA Toshiaki 日本医科大学, 医学部, 准教授 (80339374)
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| Project Period (FY) |
2009 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | グランザイム3 / モノクローナル抗体 / 酵素免疫測定法 / 細胞障害性T細胞 / バイオマーカー / 炎症 |
|---|
| Research Abstract |
A new sandwich ELISA for human granzyme 3 was established using monoclonal antibodies raised against recombinant human granzyme 3 produced in E. coli. On the other hand, human peripheral blood lymphocytes were shown to secrete enzymatically inactive precursor of granzyme 3 (progranzyme 3) to the culture medium in vitro. Therefore, new monoclonal antibodies against progranzyme 3 were prepared. Using these antibodies, we could measure active granzyme 3 and inactive progranzyme 3 separately. Granzyme 3 concentrations in human plasma samples from healthy donors were very low and less than the detection limit of the ELISA.
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