Can human plasma granzyme 3 be used as a biological marker for inflammation?
| Project/Area Number |
21590669
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Nippon Medical School |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRATA Yukiyo 日本医科大学, 医学部, 助教 (40322515)
KAWADA Tomoyuki 日本医科大学, 医学(系)研究科(研究院), 教授 (00224791)
OTSUKA Toshiaki 日本医科大学, 医学部, 准教授 (80339374)
|
|---|
| Project Period (FY) |
2009 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | グランザイム3 / モノクローナル抗体 / 酵素免疫測定法 / 細胞障害性T細胞 / バイオマーカー / 炎症 |
|---|
| Research Abstract |
A new sandwich ELISA for human granzyme 3 was established using monoclonal antibodies raised against recombinant human granzyme 3 produced in E. coli. On the other hand, human peripheral blood lymphocytes were shown to secrete enzymatically inactive precursor of granzyme 3 (progranzyme 3) to the culture medium in vitro. Therefore, new monoclonal antibodies against progranzyme 3 were prepared. Using these antibodies, we could measure active granzyme 3 and inactive progranzyme 3 separately. Granzyme 3 concentrations in human plasma samples from healthy donors were very low and less than the detection limit of the ELISA.
|
Report
(5 results)
Research Products
(22 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Expression of granzyme 3 protein in human peripheral blood lymphocytes analyzed by flow cytometry.2010
Author(s)
Inagaki, H., Hirata, Y., Shimizu, T., Kobayashi, M., Li, Q., Kawada, T.
Organizer
14th International Congress of Immunology
Place of Presentation
Kobe (Kobe International Exhibition Hall)
Year and Date
2010-08-23
Related Report
-
-
[Presentation] Expression of granzyme 3 protein in human peripheral blood lymphocytes analyzed by flow cytometry.2010
Author(s)
Inagaki, H., Hirata, Y., Shimizu, T., Kobayashi, M., Li, Q., Kawada, T.
Organizer
14th International Congress of Immunology
Place of Presentation
Kobe (Kobe International Exhibition Hall))
Year and Date
2010-08-23
Related Report
-
-
-
-
-
