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Can human plasma granzyme 3 be used as a biological marker for inflammation?

Research Project

Project/Area Number 21590669
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionNippon Medical School

Principal Investigator

INAGAKI Hirofumi  日本医科大学, 医学部, 講師 (50213111)

Co-Investigator(Kenkyū-buntansha) HIRATA Yukiyo  日本医科大学, 医学部, 助教 (40322515)
KAWADA Tomoyuki  日本医科大学, 医学(系)研究科(研究院), 教授 (00224791)
OTSUKA Toshiaki  日本医科大学, 医学部, 准教授 (80339374)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsグランザイム3 / モノクローナル抗体 / 酵素免疫測定法 / 細胞障害性T細胞 / バイオマーカー / 炎症
Research Abstract

A new sandwich ELISA for human granzyme 3 was established using monoclonal antibodies raised against recombinant human granzyme 3 produced in E. coli. On the other hand, human peripheral blood lymphocytes were shown to secrete enzymatically inactive precursor of granzyme 3 (progranzyme 3) to the culture medium in vitro. Therefore, new monoclonal antibodies against progranzyme 3 were prepared. Using these antibodies, we could measure active granzyme 3 and inactive progranzyme 3 separately. Granzyme 3 concentrations in human plasma samples from healthy donors were very low and less than the detection limit of the ELISA.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010

All Presentation (22 results)

  • [Presentation] 抗ヒトGranzyme3モノクローナル抗体による酵素活性の上昇2012

    • Author(s)
      稲垣弘文、平田幸代、清水孝子、川田智之
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡(マリンメッセ福岡)
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] FRET基質を用いたヒトGranzyme3の反応速度論的解析2012

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡(マリンメッセ福岡)
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 抗ヒトGranzyme3モノクローナル抗体の酵素活性への影響2012

    • Author(s)
      稲垣弘文、平田幸代、清水孝子、川田智之
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌(北海道大学)
    • Year and Date
      2012-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] FRET基質ライブラリを用いたヒトGranzyme3とGranzymeAの活性比較2012

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌(北海道大学)
    • Year and Date
      2012-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 抗ヒトGranzyme 3モノクローナル抗体の酵素活性への影響2012

    • Author(s)
      稲垣弘文、平田幸代、清水孝子、川田智之
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌(北海道大学)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] FRET基質ライブラリを用いたヒトGranzyme 3とGranzsyme Aの活性比較2012

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌(北海道大学)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 抗ヒトGranzyme 3モノクローナル抗体による酵素活性の上昇2012

    • Author(s)
      稲垣弘文、平田幸代、清水孝子、川田智之
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡(マリンメッセ福岡)
    • Related Report
      2012 Annual Research Report
  • [Presentation] FRET基質を用いたヒトGranzyme 3の反応速度論的解析2012

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡(マリンメッセ福岡)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト前駆体型Granzyme3に特異的なsandwichELISAの確立2011

    • Author(s)
      稲垣弘文、平田幸代、清水孝子、川田智之
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都(京都国際会館)
    • Year and Date
      2011-09-23
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒトGranzyme3の基質アミノ酸配列の検索2011

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都(京都国際会館)
    • Year and Date
      2011-09-23
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト前駆体型Granzyme 3に特異的なsandwich ELISAの確立2011

    • Author(s)
      稲垣弘文、平田幸代、清水孝子、川田智之
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都(京都国際会館)
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトGranzyme 3の基質アミノ酸配列の検索2011

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都(京都国際会館)
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトGranzyme3の基質アミノ酸配列の検索2011

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡(ツインメッセ静岡)
    • Year and Date
      2011-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒトGranzyme 3の基質アミノ酸配列の検索2011

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡(ツインメッセ静岡)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Expression of granzyme 3 protein in human peripheral blood lymphocytes analyzed by flow cytometry.2010

    • Author(s)
      Inagaki, H., Hirata, Y., Shimizu, T., Kobayashi, M., Li, Q., Kawada, T.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe (Kobe International Exhibition Hall)
    • Year and Date
      2010-08-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Establishment of a new sandwich ELISA system for human granzyme 3.2010

    • Author(s)
      Hirata, Y., Inagaki, H., Shimizu, T., Kobayashi, M., Kawada, T.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe (Kobe International Exhibition Hall)
    • Year and Date
      2010-08-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Expression of granzyme 3 protein in human peripheral blood lymphocytes analyzed by flow cytometry.2010

    • Author(s)
      Inagaki, H., Hirata, Y., Shimizu, T., Kobayashi, M., Li, Q., Kawada, T.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe (Kobe International Exhibition Hall))
    • Year and Date
      2010-08-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Establishment of a new sandwich ELISA system for human granzyme 3.2010

    • Author(s)
      Hirata, Y., Inagaki, H., Shimizu, T., Kobayashi, M., Kawada, T.
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe (Kobe International Exhibition Hall))
    • Year and Date
      2010-08-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Lymphokine-activatedkiller(LAK)細胞によるGranzyme3の産生2010

    • Author(s)
      稲垣弘文、平田幸代、清水孝子、小林麻衣子、川田智之
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山(桃太郎アリーナ)
    • Year and Date
      2010-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 活性型ヒトGranzyme3に特異的なsandwich ELISAの確立2010

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山(桃太郎アリーナ)
    • Year and Date
      2010-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Lymphokine-activated killer (LAK)細胞によるGranzyme 3の産生2010

    • Author(s)
      稲垣弘文、平田幸代、清水孝子、小林麻衣子、川田智之
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] 活性型ヒトGranzyme 3に特異的なsandwich ELISAの確立2010

    • Author(s)
      平田幸代、稲垣弘文、清水孝子、川田智之
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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