Analysis on DNA methylation profiles in forensic evidence

• Project/Area Number: 21590742
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Iwate Medical University
• Principal Investigator: NAKAYASHIKI Nori 岩手医科大学, 医学部, 講師 (10146029)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: DNAメチル化 / 経時変化 / DNA多型 / ethnicity / ハプロタイプ / H19 / 日本人特異的 / 遺伝子 / 個人識別 / エスニック由来

Research Abstract

1.Methylation status in forensic evidences under eight different environmental conditions(temperature, humidity, light etc.) was analyzed, however, no significant difference was observed.
2.A haplotype polymorphism composed of 23 SNPs upstream of the H19 gene was investigated, and 21 haplotypes including several ethnicity-specific haplotypes were observed from nine ethnic groups.

Research Products

• [Journal Article] H19インプリント遺伝子のハプロタイプ分析～2 (2011)
  • Author(s): 中屋敷徳, 出羽厚二, 湯浅勲, 山本敏充
  • Journal Title: DNA多型 Volume: 19 Pages: 151-154
  • NAID: 10029668793
• [Journal Article] The distribution of H19HP haplotypes in Asian populations (2011)
  • Author(s): Nakayashiki N, Fujita M, Dewa K, Ding M, Wei W, Wang X, Yamamoto T, Yuasa I
  • Journal Title: Forens. Sci. Int. Genet. Suppl. Ser Volume: 3
  • Peer Reviewed
• [Journal Article] The distribution of H19HP haplotypes in Asian populations (2011)
  • Author(s): Nori Nakayashiki
  • Journal Title: Forensic Science International : Genetics Supplement Series Volume: 3 Issue: 1 Pages: e341-e342
  • DOI: 10.1016/j.fsigss.2011.09.033
  • Peer Reviewed
• [Journal Article] H19インプリント遺伝子のハプロタイプ分析2 (2011)
  • Author(s): 中屋敷徳, 他
  • Journal Title: DNA多型 Volume: 19(印刷中)
  • NAID: 10029668793
• [Journal Article] Investigation of SNP haplotypes in the H19 imprinted gene (2009)
  • Author(s): Nakayashiki N, Shimamoto K, Takamiya M, Hashiyada M, Yuasa I, Dewa K
  • Journal Title: Forens. Sci. Int. Genet. Suppl. Ser Volume: 2 Pages: 540-541
  • Peer Reviewed
• [Journal Article] Investigation of SNP haplotypes in the H19 imprinted gene (2009)
  • Author(s): Nori Nakayashiki
  • Journal Title: Forens Sci Int : Genet Supplement Series 2 Pages: 540-541
  • Peer Reviewed
• [Presentation] The distribution of H19HP haplotypes in Asian populations (2011)
  • Author(s): Nakayashiki N, Fujita M, Dewa K, Ding M, Wei W, Wang X, Yamamoto T, Yuasa I
  • Organizer: The 24^<th> World Congress of the International Society for Forensic Genetics
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-09-01
• [Presentation] The distribution of H19HP haplotypes in Asian populations (2011)
  • Author(s): Nori Nakayashiki
  • Organizer: 24^<th> World Congress International Society for Forensic Genetics
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-09-01
• [Presentation] H19インプリント遺伝子のハプロタイプ分析2 (2010)
  • Author(s): 中屋敷徳, 他
  • Organizer: 第19回日本DNA多型学会学術集会
  • Place of Presentation: 三島
  • Year and Date: 2010-11-19
• [Presentation] H19インプリント遺伝子のハプロタイプ分析～2 (2010)
  • Author(s): 中屋敷徳, 出羽厚二, 湯浅勲, 山本敏充
  • Organizer: 日本DNA多型学会第19回学術集会
  • Place of Presentation: 三島
  • Year and Date: 2010-11-18
• [Presentation] H19インプリント遺伝子上流のSNPハプロタイプ分析 (2010)
  • Author(s): 中屋敷徳, 高宮正隆, 橋谷田真樹, 湯浅勲, 山本敏充, 出羽厚二
  • Organizer: 日本法医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-25
• [Presentation] H19インプリント遺伝子上流のSNP-ハプロタイプ分析 (2010)
  • Author(s): 中屋敷徳, 他
  • Organizer: 第94次日本法医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-25
• [Presentation] Investigation of SNP haplotypes in the H19 imprinted gene (2009)
  • Author(s): Nakayashiki N, Shimamoto K, Takamiya M, Hashiyada M, Yuasa I, Dewa K
  • Organizer: The 23^<rd> World Congress of the International Society for Forensic Genetics
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2009-09-18
• [Presentation] Investigation of SNP haplotypes in the H19 imprinted gene (2009)
  • Author(s): Nori Nakayashiki
  • Organizer: 23^<rd> World Congress International Society for Forensic Genetics
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2009-09-18