Immunological tolerance induced by Kampo medicine kakkonto through the regulation of helper T cell differentiation in the mucosal immune system of the intestine.

• Project/Area Number: 21590760
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: University of Toyama
• Principal Investigator: KADOWAKI Makoto 富山大学, 和漢医薬学総合研究所, 教授 (20305709)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Takeshi 富山大学, 和漢医薬学総合研究所, 助教 (70316181) ; 影山 夏子 富山大学, 和漢医薬学総合研究所, 助教 (90342865)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 東洋医学 / 腸管粘膜免疫系 / 葛根湯 / 食物アレルギー / 制御性T細胞 / 活性化T細胞 / 末梢性免疫寛容 / 樹状細胞 / Foxp3 / CD25

Research Abstract

The mechanisms underlying the pharmacological effects of kakkonto in murine food allergy model were examined. As a result, kakkonto facilitated the differentiation from naive helper T cells to CD25 negative CD3+CD4+FR4+Foxp3+T cells in the immunological responses within the mucosal immune system of the intestine and thereby alleviated the allergic responses. However, the further detailed investigation of the underlying mechanisms is needed.

Research Products

• [Journal Article] Oral Tolerance Induced by Transfer of Food Antigens via Breast Milk of Allergic Mothers Prevents Offspring from Developing Allergic Symptoms in a Mouse Food Allergy Model (2012)
  • Author(s): Yamamoto T, Tsubota Y, Kodama T, Kageyama-Yahara N, Kadowaki M
  • Journal Title: Clinical and Developmental Immunology Volume 2012(2012) Volume: 2012 Pages: 721085-721085
  • DOI: 10.1155/2012/721085
  • Peer Reviewed
• [Journal Article] Rab5a regulates surface expression of FcεRI and functional activation in mast cells (2011)
  • Author(s): Kageyama-Yahara N., Suehiro Y., Yamamoto T., Kadowaki M
  • Journal Title: Biological & Pharmaceutical Bulletin Volume: 34 Pages: 760-763
  • Peer Reviewed
• [Journal Article] Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde inhibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells (2011)
  • Author(s): Kageyama-Yahara N., Wang X., Katagiri T., Wang P., Yamamoto T., Tominaga M., Kadowaki M
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 416 Pages: 283-288
  • Peer Reviewed
• [Journal Article] Suppression of phospholipase Cγ1 phosphorylation by cinnamaldehyde in hibits antigen-induced extracellular calcium influx and degranulation in mucosal mast cells (2011)
  • Author(s): Kageyama-Yahara N, Wang X, Katagiri T, Wang P, Yamamoto T, Tominaga M, Kadowaki M
  • Journal Title: Biochem Biophys Res Commun Volume: 416 Issue: 3-4 Pages: 283-8
  • DOI: 10.1016/j.bbrc.2011.11.014
  • Peer Reviewed
• [Journal Article] Rab5a Regulates Surface Expression of Fc.EPSILON.RI and Functional Activation in Mast Cells (2011)
  • Author(s): Kageyama-Yahara N, Suehiro Y, Yamamoto T, Kadowaki M
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 34 Issue: 5 Pages: 760-763
  • DOI: 10.1248/bpb.34.760
  • NAID: 130000657792
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed
• [Journal Article] The inhibitory effect of ergosterol, a bioactive constituent of a traditional Japanese herbal medicine saireito on the activity of mucosal-type mast cells (2010)
  • Author(s): Kageyama-Yahara N., Wang P., Wang X., Yamamoto T., Kadowaki M
  • Journal Title: Biological & Pharmaceutical Bulletin Volume: 33 Pages: 142-145
  • NAID: 130000140209
  • Peer Reviewed
• [Journal Article] Pentagalloy lglucose down-regulates mast cell surface FcepsilonRI expression in vitro and in vivo (2010)
  • Author(s): Kageyama-Yahara N., Suehiro Y., Maeda F., Kageyama S., Fukuoka J., Katagiri T., Yamamoto T., Kadowaki M
  • Journal Title: FEBS Letter Volume: 584 Pages: 111-118
  • Peer Reviewed
• [Journal Article] Pentagalloylglucose down-regulates mast cell surface FcepsilonRI expression in vitro and in vivo. (2010)
  • Author(s): Kageyama-Yahara N, Suehiro Y, Maeda F, Kageyama S, Fukuoka J, Katagiri T, Yamamoto T, Kadowaki M.
  • Journal Title: FEBS Lett. Volume: 584 Pages: 111-118
  • Peer Reviewed
• [Journal Article] The inhibitory effect of ergosterol, a bioactive constituent of a traditional Japanese herbal medicine saireito on the activity of mucosal-type mast cells. (2010)
  • Author(s): Kageyama-Yahara N., Wang P., Wang X., Yamamoto T., Kadowaki M.
  • Journal Title: Biological & Pharmaceutical Bulletin Volume: 33 Pages: 142-145
  • NAID: 130000140209
  • Peer Reviewed
• [Journal Article] Pentagalloylglucose down-regulates mast cell surface FcepsilonRI expression in vitro and in vivo. (2010)
  • Author(s): Kageyama-Yahara N, Suehiro Y, Maeda F, Kageyama S, Fukuoka J, Katagiri T, Yamamoto T, Kadowaki M.
  • Journal Title: FEBS Lett. 584 Pages: 111-118
  • Peer Reviewed
• [Presentation] 樹状細胞を介した葛根湯によるエフェクターT細胞の分化・増殖の抑制 (2011)
  • Author(s): 山本武,原優加,門脇真
  • Organizer: 第28回和漢医薬学会学術大会
  • Place of Presentation: 富山
• [Presentation] 樹状細胞を介した葛根湯によるエフェクターT細胞の分化・増殖の抑制 (2011)
  • Author(s): 山本武
  • Organizer: 第28回和漢医薬学会学術大会
  • Place of Presentation: 富山県民会館
• [Presentation] 食物アレルギー病態モデルにおける葛根湯の新規制御性T細胞を介した腸管粘膜免疫抑制作用 (2010)
  • Author(s): 山本武
  • Organizer: 第27回和漢医薬学会
  • Place of Presentation: 京都薬科大学
  • Year and Date: 2010-08-29
• [Presentation] Traditional Chinese-Japanese medicine research in mucosal immunity. (2010)
  • Author(s): Kadowaki M.
  • Organizer: East-West Scientific Symposia on Allergy & Traditional Oriental Medicine
  • Place of Presentation: Luoyang, China
  • Year and Date: 2010-04-16
• [Presentation] Traditional Chinese-Japanese medicine research in mucosal immunity (2010)
  • Author(s): Kadowaki M
  • Organizer: East-West Scientific Symposia on Allergy & Traditional Oriental Medicine
  • Place of Presentation: Luoyang, China
• [Presentation] 食物アレルギー病態モデルにおける葛根湯の新規制御性T細胞を介した腸管粘膜免疫抑制作用 (2010)
  • Author(s): 山本武,藤原加苗,坪田悠真,門脇真
  • Organizer: 第27回和漢医薬学会学術大会
  • Place of Presentation: 京都
• [Presentation] 食物アレルギー性消化器症状誘発病態モデルにおける葛根湯による制御性T細胞への分化亢進を介する腸管粘膜免疫系の制御機構 (2009)
  • Author(s): 山本武
  • Organizer: 第26回和漢医薬学会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2009-08-29
• [Presentation] Effect of kakkonto, a traditional Japanese herbal medicine, on the differentiation of CD4+T cells in the colon of food allergy model mouse (2009)
  • Author(s): 門脇真
  • Organizer: 国際粘膜免疫学会
  • Place of Presentation: ボストン アメリカ合衆国
  • Year and Date: 2009-07-07
• [Presentation] Effect of kakkonto, a traditional Japanese herbal medicine, on the differentiation of CD4+T cells in the colon of food allergy model mouse (2009)
  • Author(s): Yamamoto T., Fujiwara K., Yoshida M., Kadowaki M
  • Organizer: International Congress of Mucosal Immunology
  • Place of Presentation: Boston, U. S. A.
• [Presentation] 食物アレルギー性消化器症状誘発病態モデルにおける葛根湯による制御性T細胞への分化亢進を介する腸管粘膜免疫系の制御機構(プレナリーセッション講演) (2009)
  • Author(s): 山本武,藤原加苗,氣賀澤愛,吉田益奈子,門脇真
  • Organizer: 第26回和漢医薬学会学術大会
  • Place of Presentation: 千葉