Evaluation of endogenous retroviral genes at human cholestatic liver diseases

• Project/Area Number: 21590822
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tohoku University
• Principal Investigator: UENO Yoshiyuki 東北大学, 大学院・医学系研究科, 非常勤講師 (70282126)
• Co-Investigator(Kenkyū-buntansha): FUKUSHIMA Koji 東北大学, 病院, 助教 (20400476)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 肝臓学 / 原発性胆汁性肝硬変 / 次世代シーケンサー / レトロウイルス / プロファイリング / 胆管上皮細胞 / 胆汁うっ滞 / モデルマウス / ORF

Research Abstract

The pathogenesis of human cholestatic liver diseases remains to be unknown. Recently, the involvement of endogenous retroviral genes is reported in various human autoimmune diseases. In this study, we evaluated these genes in human samples as well as animal model. We detected some retroviral derived genes in female mouse liver. Furthermore, we have done several conditioning experiments using sensitive deep sequencing analyzer.

Research Products

• [Journal Article] PBC : Animal Models of Cholangiopathies and Possible Endogenous Viral Infections (2012)
  • Author(s): Ninomiya M, Ueno Y, Shimosegawa T
  • Journal Title: International journal of hepatology Volume: 2012 Pages: 649290-649290
  • Peer Reviewed
• [Journal Article] Use of illumina deep sequencing technology to differentiate hepatitis C virus variants (2012)
  • Author(s): Ninomiya M, Ueno Y, Funayama R, Nagashima T, Nishida Y, Kondo Y, Inoue J, Kakazu E, Kimura O, Nakayama K, Shimosegawa T
  • Journal Title: Journal of clinical microbiology Volume: 50(3) Pages: 857-866
  • Peer Reviewed
• [Journal Article] Role of stem cell factor and granulocyte colony-stimulating factor in remodeling during liver regeneration (2012)
  • Author(s): Meng F, Francis H, Glaser S, Han Y, DeMorrow S, Stokes A, Staloch D, Venter J, White M, Ueno Y, Reid LM, Alpini G
  • Journal Title: Hepatology Volume: 55(1) Pages: 209-221
  • Peer Reviewed
• [Journal Article] Use of illumina deep sequencing technology to differentiate hepatitis Cvirus variants (2012)
  • Author(s): Ninomiya M, Ueno Y, Funayama R, Kagashima T, Nishida Y, Kondo Y, Inoue J, Kakazu E, Kimura O, Nakayama K, Shimosegawa T
  • Journal Title: Journal of clinical microbiology Volume: 50 Issue: 3 Pages: 857-866
  • DOI: 10.1128/jcm.05715-11
  • Peer Reviewed
• [Journal Article] Animal Models of Cholangiopathies and Possible Endogenous Viral Infecti (2012)
  • Author(s): Ninomiya M, Ueno Y, Shimosegawa T
  • Journal Title: International journal of hepatology Volume: 2012 Pages: 649290-649290
  • DOI: 10.1155/2012/649290
  • Peer Reviewed
• [Journal Article] Knockout of the neurokinin-1 receptor reduces cholangiocyte proliferation in bile duct-ligated mice (2011)
  • Author(s): Glaser S, Gaudio E, Renzi A, Mancinelli R, Ueno Y, Venter J, White M, Kopriva S, Chiasson V, Demorrow S, Francis H, Meng F, Marzioni M, Franchitto A, Alvaro D, Supowit S, Dipette DJ, Onori P, Alpini G
  • Journal Title: American journal of physiology Gastrointestinal and liver physiology
  • Peer Reviewed
• [Journal Article] Adenosine triphosphate release and purinergic(P2)receptor-mediated secretion in small and large mouse cholangiocytes (2010)
  • Author(s): Woo K, Sathe M, Kresge C, Esser V, Ueno Y, Venter J, Glaser SS, Alpini G, Feranchak AP
  • Journal Title: Hepatology Pages: 1819-1828
  • Peer Reviewed
• [Journal Article] Murine models of autoimmune cholangitis. Curr Opin Gastroenterol (2010)
  • Author(s): Ueno Y, Ambrosini YM, Moritoki Y, Ridgway WM, Gershwin ME
  • Journal Title: Curr Opin Gastroenterol Pages: 274-279
  • Peer Reviewed
• [Journal Article] Treatment of Primary Biliary Cirrhosis : A new challenge? (2010)
  • Author(s): Fukushima K, Ueno Y, Shimosegawa T
  • Journal Title: Hepatology research : the official journal of the Japan Society of Hepatology Volume: 40(1) Pages: 61-68
  • NAID: 10027406756
  • Peer Reviewed
• [Journal Article] Adenosine triphosphate release and purinergic (P2) receptor-mediated secretion in small and large mouse cholangiocytes. (2010)
  • Author(s): Woo K., Sathe M., Kresge C., Esser V., Ueno Y., Venter J., Glaser S.S., Alpini G. Feranchak A.P.
  • Journal Title: Hepatology Volume: 52 Pages: 1819-28
  • Peer Reviewed
• [Journal Article] Murine models of autoimmune cholangitis. (2010)
  • Author(s): Ueno Y., Ambrosini Y.M., Moritoki Y., Ridgway W.M., Gershwin M.E.
  • Journal Title: Curr Opin Gastroenterol Volume: 26 Pages: 274-9
  • Peer Reviewed
• [Journal Article] Knockout of secretin receptor reduces large cholangiocyte hyperplasia in mice with extrahepatic cholestasis induced by bile duct ligation. (2010)
  • Author(s): Glaser S., Lam I.P., Franchitto A., Gaudio E., Onori P., Chow B.K., Wise C., Kopriva S., Venter J., Whitc M., Ucno Y., Dostal D., Carpino G., Mancinelli R., Butler W., Chiasson V., DeMorrow S., Francis H., Alpini G.
  • Journal Title: Hepatology Volume: 52 Pages: 204-14
  • Peer Reviewed
• [Journal Article] Treatment of Primary Biliary Cirrhosis : A new challenge? (2010)
  • Author(s): Fukushima K, Ueno Y, Shimosegawa T.
  • Journal Title: Hepatol Res 40 Pages: 61-68
  • NAID: 10027406756
  • Peer Reviewed
• [Journal Article] Compensatory role of inducible annexin A2 for impaired biliary epithelial anion-exchange activity of inflammatory cholangiopathy (2009)
  • Author(s): Kido O, Fukushima K, Ueno Y, Inoue J, Jefferson DM, Shimosegawa T
  • Journal Title: Laboratory investigation Pages: 1374-1386
  • Peer Reviewed
• [Journal Article] Compensatory role of inducible annexin A2 for impaired biliary epithelial anion-exchange activity of inflammatory cholangiopathy. (2009)
  • Author(s): Kido O, Fukushima K, Ueno Y, Inoue J, Jefferson DM, Shimosegawa T.
  • Journal Title: Lab Invest 89 Pages: 1374-1386
  • Peer Reviewed
• [Presentation] H1 and H2 Histamine Receptor Agonists Differentially Stimulate the Regeneration of Small and Large Cholangiocytes by Activation of Ca(2+)and Camp-Dependent Mechanisms, Respectively (2011)
  • Author(s): Francis HL, Meng F, Onori P, Venter J, White M, Ueno Y, Franchitto A, Renzi A, Gaudio E, Alpini G
  • Organizer: San Francisco
  • Place of Presentation: 米国
  • Year and Date: 2011-11-07
• [Presentation] H1 and H2 Histamine Receptor Agonists Differentially Stimulate the Regeneration of Small and Large Cholangiocytes by Activation of Ca (2+) and Camp-Dependent Mechanisms, Respectively (2011)
  • Author(s): Francis HL, Meng F, Onori P, Venter J, White M, Ueno Y, Franchitto A, Renzi A, Gaudio E, Alpini G
  • Organizer: Annual Meeting of American Association for the Study of Liver Diseases
  • Place of Presentation: Boston,米国
  • Year and Date: 2011-11-07