• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of hepatic encephalopathy induced proteins using by a bio-artificial liver and toxin receptor knock out hepatitis model mice

Research Project

Project/Area Number 21590862
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionJikei University School of Medicine

Principal Investigator

MATSUURA Tomokazu  東京慈恵会医科大学, 医学部, 准教授 (30199749)

Co-Investigator(Renkei-kenkyūsha) KIMURA Naofumi  東京慈恵会医科大学, 医学部, 教授 (80138742)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords肝臓学 / 肝性脳症 / バイオ人工肝臓 / 肝炎モデルマウス / 肝炎モデル / マウス / ジフテリア毒素
Research Abstract

Identification of unknown hepatic encephalopathy induced substances to cause the "rapid cerebral edema" in fulminant hepatitis is required in development of the treatment and diagnosis of fatal hepatic encephalopathy. The main purpose of this study is identification of brain astrocytes damage inducing plasma proteins. A candidate of fatal hepatic encephalopathy inducing proteins, temporary named protein A, was able to identify by proteomic analysis. Protein A induced the cytotoxicity to cultured astrocytes. Hepatic encephalopathy was induced in the TRECK hepatitis model mice. In addition, we could observe diffuse astrocyte swelling in acute hepatic encephalopathy by DWI on MRI, as a decrease in the ADC. We will study whether protein A induce hepatic encephalopathy in vivo model and clinical cases in next step, and promote the treatment for fatal encephalopathy.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (12 results)

All 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (2 results) Book (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Transplantation of liver organoids in the omentum and kidney2011

    • Author(s)
      Saito R、Ishii Y、Ito R、Nagatsuma K、Tanaka K、Saito M、Maehashi H、Nomoto H、Ohkawa K、Mano H、Aizawa M、Hano H、Yanaga K、Matsuura T
    • Journal Title

      Artif Organs

      Volume: Vol.35, No.1 Issue: 1 Pages: 80-83

    • DOI

      10.1111/j.1525-1594.2010.01049.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transplantation of liver organoids in the omentum and kidney.2011

    • Author(s)
      alto R, Ishii Y, Ito R, Nagatsuma K, Tanaka K, Saito M, Maehashi H, Nomoto H, Ohkawa K, Mano M, Aizawa M, Hano H, Yanaga K, Matsuura T.
    • Journal Title

      Artif Organs.

      Volume: 35 Pages: 80-83

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transplantation of liver organoids in the omentum and kidney2011

    • Author(s)
      Saito R, Ishii Y, Ito R, Nagatsuma K, Tanaka K, Saito M, Mehashi H, Matsuura T, et al.
    • Journal Title

      Artif Organs (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ^<13> Cグルコース呼気試験を利用した3次元還流培養系の活性測定とその応用2010

    • Author(s)
      田中賢、松浦知和、中田浩二
    • Journal Title

      Radioisotope

      Volume: Vol.59, No.7 Pages: 435-440

    • NAID

      10026799885

    • Related Report
      2011 Final Research Report
  • [Journal Article] ^<13>Cグルコース呼気試験を利用した3次元還流培養系の活性測定とその応用2010

    • Author(s)
      田中賢、松浦知和、中田浩二
    • Journal Title

      Radioisotopes

      Volume: 59 Pages: 435-440

    • NAID

      10026799885

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Three-dimensional culture using a radial flow bioreactor induces matrix metalloprotease 7-mediated EMT-like process in tumor cells via TGFβ1/Smad pathway2009

    • Author(s)
      Shibata S, Marushima H, Asakura T, Matsuura T, Eda H, Aoki K, Matsudaira H, Ueda K, Ohkawa K
    • Journal Title

      Int J Oncol

      Volume: Vol.34, No.5 Pages: 1433-1448

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensuonal culture using a radialflow bioreactor induces matrix metalloprotease 7-mediated EMT-like process in tumor cells via TGFβ1/Smad pathway.2009

    • Author(s)
      Shibata S, Marushima H, Asakura T, Matsuura T, Eda H, Aoki K, et al.
    • Journal Title

      Int J Oncol 34

      Pages: 1433-1448

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] (ワークショップ)肝再生医学臨床応用を目指した研究の新展開類洞類似構造を再現した肝臓オルガノイドの作製2010

    • Author(s)
      松浦知和、石井雄二、相澤守
    • Organizer
      第46回日本肝臓学会総会
    • Place of Presentation
      山形
    • Year and Date
      2010-05-28
    • Related Report
      2011 Final Research Report
  • [Presentation] (ワークショップ)肝再生医学 臨床応用を目指した研究の新展開、類洞類似構造を再現した肝臓オルガノイドの作製2010

    • Author(s)
      松浦知和, 石井雄二, 相澤守
    • Organizer
      第46回日本肝臓学会総会
    • Place of Presentation
      山形市
    • Year and Date
      2010-05-28
    • Related Report
      2010 Annual Research Report
  • [Book] バイオリアクターシステム2009

    • Author(s)
      松浦知和, 他
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Patent(Industrial Property Rights)] ガス混合装置及びガス混合方法2011

    • Inventor(s)
      藤原邦彦、松浦知和
    • Industrial Property Rights Holder
      エイブル(株)、松浦知和、大川清
    • Acquisition Date
      2011-11-25
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] バイオリアクター2010

    • Inventor(s)
      相澤守、松浦知和
    • Industrial Property Rights Holder
      学校法人明治大学
    • Acquisition Date
      2010-11-26
    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi