The investigation of the effect of transplantation of neural crest derived cells to murine focal cerebral ischemia model
Project/Area Number |
21591119
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Neurology
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Research Institution | Keio University |
Principal Investigator |
TOMITA Yutaka 慶應義塾大学, 医学部, 講師(非常勤) (60276251)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 神経堤細胞 / 脳梗塞 / Confocal顕微鏡 / Matlab software / Matlab Software |
Research Abstract |
The aim of the study was to monitor in vivo and noninvasively the fate of neural crest cells transplanted into the ischemic brain of unirradiated mice. In vivo imaging was performed through a closed cranial window following cell transplantation, using laser-scanning confocal fluorescence microscopy. Repeated in vivo imaging revealed that transplanted cells survived within the ischemic and peri-ischemic cortex, migrated significantly towards the lesion, proliferated. This study indicates that confocal fluorescence microscopy is a reliable and unique tool to repeatedly assess with cellular resolution the in vivo dynamic fate of fluorescent cells transplanted into a mouse brain.
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Report
(4 results)
Research Products
(81 results)
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[Journal Article] Artificial RBC transiently improved microcirculatory derangement produced by blood-loss hypotension or MCA occlusion in mice2009
Author(s)
Tomita Y, Toriumi H, Hattori H, Unekawa M, Tomita M, Itoh Y, Hoshino H, Sakai H, Tsuchida E, Horinouchi H, Kobayashi K, Suzuki N
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Journal Title
J.Cereb.Blood Flow Metab. 29
Related Report
Peer Reviewed
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[Presentation] Effect of injection of artificial rbcs on murine hemorrhagic hypotension model2011
Author(s)
Tomita Y, Unekawa M, Toriumi H, Masamoto K, Sakai H, Tsuchida E, Horinouchi H, Kobayashi K, Kanno I, Suzuki N.
Organizer
XIII International Symposium on Blood Substitutes and Oxygen Therapeutics
Place of Presentation
Boston, USA
Year and Date
2011-07-29
Related Report
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[Presentation] The effect of Artificial RBCS injection for hemorrhagic hypotension in mice2011
Author(s)
Tomita Y, Unekawa M, Toriumi H, Masamoto K, Sakai H, Tsuchida E, Horinouchi H, Kobayashi K, Kanno I, Suzuki N
Organizer
Brain 11 ; 25th International Symposium on Cerebral Blood Flow, Metabolism & Function
Place of Presentation
Barcelona
Year and Date
2011-05-28
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[Presentation] Artificial RBCs Transiently improved microcirculatory derangement produced by MCA occlusion or hemorrhagic hypotension in mice2009
Author(s)
Tomita Y, Toriumi H, Hattori H, Unekawa M, Tomita M, Itoh Y, Hoshino H, Sakai H, Tsuchida E, Horinouchi H, Kobayashi K, Suzuki N
Organizer
Brain 09
Place of Presentation
Chicago
Year and Date
2009-07-02
Related Report
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[Presentation] Artificial RBC transiently improved microcirculatory derangement produced by blood-loss hypotension or MCA occlusion in mice2009
Author(s)
Tomita Y, Toriumi H, Hattori H, Unekawa M, Tomita M, Itoh Y, Hoshino H, Sakai H, Tsuchida E, Horinouchi H, Kobayashi K, Suzuki N
Organizer
Brain 09
Place of Presentation
Chicago
Related Report
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