Regulation of carbohydrate and lipid metabolism in regard to purine metabolism in skeletal muscle

• Project/Area Number: 21591167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: MORISAKI Takayuki 独立行政法人国立循環器病研究センター, 分子生物学部, 部長 (30174410)
• Co-Investigator(Renkei-kenkyūsha): MORISAKI Hiroko 独立行政法人国立循環器病研究センター, 分子生物学部, 室長 (40311451)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: プリン代謝 / 骨格筋 / 糖代謝 / 脂質代謝 / AMP / AMPK / アデニンヌクレオチド / 代謝調節 / 糖脂質代謝 / ヌクレオチド代謝 / 遺伝子改変動物 / 代謝異常

Research Abstract

In this study, we revealed changes in nucleotide levels, phosphorylated AMPK and other relating molecules in AMPD1 knock-out(muscle AMPD deficient) mice, indicating the close relationship between adenine nucleotide and carbohydrate/lipid metabolism. Also, we studied the relationship between AMPD deficiency and renal glomerular function.

Research Products

• [Journal Article] Proteinuria in AMPD2-deficient mice (2012)
  • Author(s): Toyama K, et al
  • Journal Title: Genes to Cells Volume: 17 Issue: 1 Pages: 28-38
  • DOI: 10.1111/j.1365-2443.2011.01568.x
  • Peer Reviewed
• [Journal Article] CDH13 gene coding T-cadherin influences variations in plasma adiponectin levels in Japanese population (2012)
  • Author(s): Morisaki H, et al
  • Journal Title: Human Mutation Volume: 33 Issue: 2 Pages: 402-410
  • DOI: 10.1002/humu.21652
  • Peer Reviewed
• [Journal Article] Establishment and analysis of SLC22A12 (URAT1) knockout mouse (2010)
  • Author(s): Hosoyamada M, Takiue Y, Morisaki H, Cheng J, Ikawa M, Okabe M, Morisaki T, Ichida K, Hosoya T, Shibasaki T
  • Journal Title: Nucleosides Nucleotides Nucleic Acids Volume: 29 Issue: 4-6 Pages: 314
  • DOI: 10.1080/15257771003738634
• [Journal Article] Establishment and analysis of SLC22A12(URAT1)knockout mouse (2010)
  • Author(s): Hosoyamada M, et al
  • Journal Title: Nucleosides Nucleotides Nucleic Acids Volume: 29 Pages: 314-320
  • Peer Reviewed
• [Presentation] AMPD2遺伝子と腎糸球体機能 (2012)
  • Author(s): 遠山桂子,森崎裕子,程継東,森崎隆幸
  • Organizer: 第45回日本痛風・核酸代謝学会総会
  • Place of Presentation: 奈良
• [Presentation] AMPD2遺伝子と腎糸球体機能 (2012)
  • Author(s): 遠山桂子, 他
  • Organizer: 第45回日本痛風・核酸代謝学会総会
  • Place of Presentation: 奈良市
• [Presentation] Murine AMPD3 deficiency : Elevation of erythrocyte ATP does not improve anemia due to PK deficiency (2011)
  • Author(s): Morisaki T, Cheng J, Toyama K, Ikawa M, Okabe M, Morisaki H
  • Organizer: 14th International Symposium on Purine and Pyrimidine Metabolism in Man
  • Place of Presentation: Tokyo
• [Presentation] Murine AMPD3 deficiency : Elevation of erythrocyte ATP does not improve anemia due to PK deficiency (2011)
  • Author(s): Morisaki T, et al
  • Organizer: 14^<th> International Symposium on Purine and Pyrimidine Metabolism in Man
  • Place of Presentation: Tokyo
• [Presentation] AMPD2 plays an important role for regulation of lipid and glucose metabolism (2010)
  • Author(s): Morisaki T, Cheng J, Toyama T, Hirase T, Ikawa M, Okabe M, Morisaki H
  • Organizer: 60th Annual Meeting of American Society of Human Genetics
  • Place of Presentation: Washington, DC
• [Presentation] 骨格筋AMPD欠損モデルマウスの樹立とその核酸代謝変化と糖脂質代謝変化 (2010)
  • Author(s): 森崎隆幸,新谷拓也,程継東,森崎裕子
  • Organizer: 第42回日本痛風・核酸代謝学会総会
  • Place of Presentation: 大阪
• [Presentation] AMPD2 plays an important role for regulation of lipid and glucose metabolism (2010)
  • Author(s): Morisaki T, et al
  • Organizer: 60th Annual Meeting of American Society of Human Genetics
  • Place of Presentation: Washington, DC, USA
• [Presentation] 骨格筋AMPD欠損モデルマウスの樹立とその核酸代謝・糖脂質代謝の変化 (2010)
  • Author(s): 森崎隆幸, 他
  • Organizer: 第43回日本痛風・核酸代謝学会
  • Place of Presentation: 大阪市
• [Presentation] 骨格筋AMPD欠損モデルマウスの樹立と代謝変化・シグナル変化の検討 (2009)
  • Author(s): 新谷拓也,森崎裕子,程継東,杉本直美,森崎隆幸
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] AMPD3 Deficient Mice Exhibit Increased Erythrocyte ATP but Do Not Improve Anemia due to PK Deficiency (2009)
  • Author(s): T. Morisaki, J. Cheng, K. Toyama, M. Ikawa, M. Okabe, H. Morisaki
  • Organizer: 59th Annual Meeting of American Society of Human Genetics
  • Place of Presentation: Honolulu
• [Presentation] Establishment of murine model for myoadenylate deaminase deficiency (2009)
  • Author(s): Takayuki Morisaki, Jidong Cheng, Naomi Sugimoto, Atsushi Dohi, Erika Kimura, Takuya Shintani, Masahito Ikawa, Masaru Okabe, Hiroko Morisaki
  • Organizer: 13th International Symposium on Purine and Pyrimidine Metabolism in Man
  • Place of Presentation: Stockholm, Sweden
• [Presentation] Establishment of murine model for myoadenylate deaminase deficiency (2009)
  • Author(s): Morisaki T, et al
  • Organizer: 13^<th> International Symposium on Purine and Pyrimidine Metabolism in Man, PP09
  • Place of Presentation: Stockholm, Sweden
• [Presentation] AMPD3 Deficient Mice Exhibit Increased Erythrocyte ATP Level but Do Not Improve Anemia due to PK Deficiency (2009)
  • Author(s): Morisaki T, et al
  • Organizer: 59^<th> Annual Meeting of the American Society of Human Genetics
  • Place of Presentation: Honolulu, Hawaii, USA
• [Book] 循環器病研究の進歩XXXII (2011)
  • Author(s): 森崎隆幸,程継東,森崎裕子
• [Book] アデニンヌクレオチド代謝と心血管疾患循環器病研究の進歩XXX II,54-64,2011 (2011)
  • Author(s): 森崎隆幸, 程継東, 森崎裕子
  • Total Pages: 11
  • Publisher: 協和企画
• [Book] 綜合臨床 (2010)
  • Author(s): 森崎隆幸,森崎裕子
• [Book] 綜合臨床 特集:痛風・高尿酸血症をめぐって 尿酸の代謝経路(59巻184-189頁) (2010)
  • Author(s): 森崎隆幸、森崎裕子
  • Total Pages: 6
  • Publisher: 永井書店