Physiological and pathological functions of Favine

• Project/Area Number: 21591178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Osaka University
• Principal Investigator: FUKUHARA Atsunori 大阪大学, 医学系研究科, 助教 (00437328)
• Co-Investigator(Kenkyū-buntansha): MAEDA Kazuhisa 大阪大学, 医学系研究科, 寄付講座准教授 (60397750)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 生活習慣病 / ホルモン / Favine / Z5

Research Abstract

We searched for novel gene with signal sequences that are specifically expressed in murine aorta, designated one gene as Favine. Favine was expressed abundantly in the aorta and adipose tissues, and its mRNA levels were higher in adipose tissues of obese db/ db mice than control mice.
In this study, we generated transgenic mouse overexpressing Favine in liver, and microarray analysis were performed in liver, adipose tissue, and aorta. In aorta of transgenic mouse, expression levels of genes associating muscle contraction were significantly increased. Moreover, in human aortic smooth muscle cells, expression levels of these genes were also induced by addition of recombinant Favine conditioned media. These results indicated that Favine should regulate aortic smooth muscle contraction. To measure Favine levels in human serum, we generated antiobody and ELISA assay system. Favine protein was detected in human serum, and its level changed depending on feeding conditions.

Research Products

• [Journal Article] Identification of a new secretory factor, CCDC3/Favine, in adipocytes and endothelial cells. (2010)
  • Author(s): Kobayashi S, Fukuhara A, Taguchi T, Matsuda M, Tochino Y, Otsuki M, Shimomura I.
  • Journal Title: Biochem Biophys Res Commun Volume: 392 Pages: 29-35
  • Peer Reviewed
• [Presentation] 血管および脂肪組織由来新規分泌因子favineの機能解析 (2011)
  • Author(s): 小林祥子, 福原淳範, 田口貴史, 大月道夫, 下村伊一郎
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-19
• [Presentation] 血管および脂肪組織由来新規分泌因子Favineの機能解析 (2011)
  • Author(s): 小林祥子
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-19
• [Presentation] 血管および脂肪組織由来新規分泌因子の同定 (2010)
  • Author(s): 小林祥子, 福原淳範, 松田守弘, 大月道夫, 下村伊一郎
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2010-05-29
• [Presentation] キーストンシンポジア (2010)
  • Author(s): 小林祥子, 福原淳範, 田口貴史, 大月道夫, 下村伊一郎
  • Organizer: Identification of a new secretory factor, CCDC3/ Favine, in adipocytes and endothelial cells
  • Place of Presentation: カナダ
  • Year and Date: 2010-04-14